Jeotgalibacillus campisalis
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3729 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C2R024|A0A0C2R024_9BACL Uncharacterized protein OS=Jeotgalibacillus campisalis OX=220754 GN=KR50_31850 PE=4 SV=1
MM1 pKa = 7.47 LKK3 pKa = 10.13 KK4 pKa = 10.56 VLLIVSIVLTGAVVYY19 pKa = 9.46 FGQDD23 pKa = 2.49 AWKK26 pKa = 10.24 DD27 pKa = 3.63 SQKK30 pKa = 10.44 EE31 pKa = 3.99 VHH33 pKa = 6.63 RR34 pKa = 11.84 SSSSVVEE41 pKa = 4.25 STSNANSEE49 pKa = 4.33 DD50 pKa = 3.82 SADD53 pKa = 3.49 SSTSIEE59 pKa = 4.1 NEE61 pKa = 4.22 SKK63 pKa = 10.62 KK64 pKa = 10.97 GSADD68 pKa = 3.48 EE69 pKa = 5.23 LEE71 pKa = 4.9 DD72 pKa = 5.55 LIANQPQDD80 pKa = 3.49 VQEE83 pKa = 4.12 FWIEE87 pKa = 4.2 SKK89 pKa = 10.99 EE90 pKa = 4.24 SGDD93 pKa = 3.7 TVDD96 pKa = 3.05 ITFVATEE103 pKa = 3.97 SAVALEE109 pKa = 4.64 EE110 pKa = 3.92 NWTTLIEE117 pKa = 4.03 EE118 pKa = 4.7 SFLSSYY124 pKa = 10.77 EE125 pKa = 4.28 GIDD128 pKa = 3.49 FAFSLITHH136 pKa = 6.19 EE137 pKa = 4.76 NEE139 pKa = 4.03 GTSEE143 pKa = 4.03 DD144 pKa = 4.2 WLTSLQSEE152 pKa = 4.21 AVTFEE157 pKa = 3.93 GQDD160 pKa = 2.88 IVLYY164 pKa = 8.32 EE165 pKa = 4.25 LPVINDD171 pKa = 3.13 NGMLSSQDD179 pKa = 3.11 QIYY182 pKa = 7.64 YY183 pKa = 8.64 TNRR186 pKa = 11.84 FLEE189 pKa = 4.48 EE190 pKa = 3.94 MQSNFPEE197 pKa = 4.2 THH199 pKa = 6.85 LFTLPSQPLYY209 pKa = 10.99 NSTYY213 pKa = 10.17 YY214 pKa = 10.01 PGEE217 pKa = 4.27 LEE219 pKa = 4.29 TVQEE223 pKa = 4.44 VVEE226 pKa = 4.2 EE227 pKa = 3.86 QGIPFLNHH235 pKa = 5.46 WEE237 pKa = 4.14 DD238 pKa = 3.3 WPSIDD243 pKa = 6.06 DD244 pKa = 4.16 EE245 pKa = 4.28 EE246 pKa = 5.63 LEE248 pKa = 5.33 NYY250 pKa = 8.17 LTDD253 pKa = 5.31 DD254 pKa = 3.84 NDD256 pKa = 3.95 PNEE259 pKa = 4.59 EE260 pKa = 4.39 GNTIWGTYY268 pKa = 8.99 LVDD271 pKa = 3.67 YY272 pKa = 10.48 FSTNN276 pKa = 2.7
Molecular weight: 31.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.743
IPC2_protein 3.668
IPC_protein 3.643
Toseland 3.452
ProMoST 3.783
Dawson 3.617
Bjellqvist 3.77
Wikipedia 3.503
Rodwell 3.478
Grimsley 3.363
Solomon 3.605
Lehninger 3.554
Nozaki 3.719
DTASelect 3.872
Thurlkill 3.49
EMBOSS 3.516
Sillero 3.757
Patrickios 1.125
IPC_peptide 3.605
IPC2_peptide 3.745
IPC2.peptide.svr19 3.704
Protein with the highest isoelectric point:
>tr|A0A0C2VPM0|A0A0C2VPM0_9BACL RNA 2' 3'-cyclic phosphodiesterase OS=Jeotgalibacillus campisalis OX=220754 GN=KR50_30610 PE=3 SV=1
MM1 pKa = 7.34 LLSWVVPRR9 pKa = 11.84 IKK11 pKa = 10.66 ASLHH15 pKa = 4.74 KK16 pKa = 10.48 QGAIFMRR23 pKa = 11.84 FFNLKK28 pKa = 9.91 LKK30 pKa = 10.72 RR31 pKa = 11.84 PVQARR36 pKa = 11.84 QVV38 pKa = 3.21
Molecular weight: 4.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.356
IPC2_protein 10.774
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.34
Grimsley 12.544
Solomon 12.983
Lehninger 12.881
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.106
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.03
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3729
0
3729
1041369
37
2211
279.3
31.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.313 ± 0.045
0.641 ± 0.013
5.221 ± 0.036
7.814 ± 0.054
4.53 ± 0.037
6.945 ± 0.053
2.169 ± 0.02
7.477 ± 0.046
6.392 ± 0.039
9.81 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.85 ± 0.022
4.082 ± 0.028
3.657 ± 0.024
3.835 ± 0.029
4.118 ± 0.028
6.432 ± 0.033
5.45 ± 0.055
6.869 ± 0.035
1.087 ± 0.017
3.309 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here