Tropicibacter phthalicicus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Tropicibacter

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4708 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A238JC85|A0A238JC85_9RHOB RHH_4 domain-containing protein OS=Tropicibacter phthalicicus OX=1037362 GN=TRP8649_02426 PE=4 SV=1
MM1 pKa = 7.33PTINHH6 pKa = 7.13DD7 pKa = 3.57PTALTTSQGDD17 pKa = 3.44VTSAHH22 pKa = 6.47FGSNFLFDD30 pKa = 4.05QDD32 pKa = 4.39FNPDD36 pKa = 2.97GTIEE40 pKa = 4.28GNWYY44 pKa = 9.89DD45 pKa = 3.79QVTDD49 pKa = 3.68AAHH52 pKa = 5.58TQTLRR57 pKa = 11.84FPGGTITEE65 pKa = 4.42TQFDD69 pKa = 4.67LNDD72 pKa = 3.62TAKK75 pKa = 10.92LEE77 pKa = 4.23EE78 pKa = 4.09FLSYY82 pKa = 10.81CSANGHH88 pKa = 6.36KK89 pKa = 10.72AMIVLPTFRR98 pKa = 11.84FFDD101 pKa = 4.47PDD103 pKa = 3.84SPNSNYY109 pKa = 10.33LEE111 pKa = 4.38SDD113 pKa = 3.22AEE115 pKa = 4.33TIIKK119 pKa = 10.58NFVQTVLDD127 pKa = 4.46LADD130 pKa = 3.78AQNPKK135 pKa = 10.48VIIEE139 pKa = 4.02GFEE142 pKa = 3.8IGNEE146 pKa = 4.06YY147 pKa = 8.95YY148 pKa = 10.13QKK150 pKa = 11.26KK151 pKa = 7.7FTNEE155 pKa = 3.54DD156 pKa = 3.41DD157 pKa = 4.81LLFGDD162 pKa = 4.27EE163 pKa = 4.98DD164 pKa = 4.15NPFVADD170 pKa = 5.08EE171 pKa = 4.1DD172 pKa = 4.85TPWTTEE178 pKa = 3.46QFARR182 pKa = 11.84LQAQMASWVNDD193 pKa = 3.23VVADD197 pKa = 3.79NVGVYY202 pKa = 8.69VQAGRR207 pKa = 11.84NSDD210 pKa = 3.93EE211 pKa = 4.13NDD213 pKa = 3.17TLYY216 pKa = 11.35GGFADD221 pKa = 5.08AGIGLDD227 pKa = 3.9VIDD230 pKa = 5.06GVLTHH235 pKa = 7.12LYY237 pKa = 9.84YY238 pKa = 10.32PPEE241 pKa = 4.14NRR243 pKa = 11.84DD244 pKa = 3.1LSVIEE249 pKa = 4.13DD250 pKa = 3.59VEE252 pKa = 4.34VRR254 pKa = 11.84IEE256 pKa = 4.14TIRR259 pKa = 11.84EE260 pKa = 3.68VWGSSRR266 pKa = 11.84EE267 pKa = 4.15LIVTEE272 pKa = 4.45WNTSEE277 pKa = 3.84ANNYY281 pKa = 8.85GGNNAVYY288 pKa = 10.69GMARR292 pKa = 11.84LAALTRR298 pKa = 11.84GFADD302 pKa = 5.17FIGHH306 pKa = 6.75GVDD309 pKa = 5.17QLLIWSTIAGGPNGRR324 pKa = 11.84ATLGLHH330 pKa = 5.04TTAEE334 pKa = 4.33QVFTPTGYY342 pKa = 9.79WFRR345 pKa = 11.84LMASEE350 pKa = 6.22LIDD353 pKa = 3.56TQLVSDD359 pKa = 4.31YY360 pKa = 11.16GSASRR365 pKa = 11.84LLDD368 pKa = 3.81AQDD371 pKa = 3.52EE372 pKa = 4.77FVGYY376 pKa = 10.36SYY378 pKa = 11.46VFQGTTFEE386 pKa = 4.38TSNDD390 pKa = 3.32YY391 pKa = 11.01HH392 pKa = 8.41LLYY395 pKa = 11.26SNGTNGDD402 pKa = 3.55LTLDD406 pKa = 3.96LDD408 pKa = 5.09LSNLMTNDD416 pKa = 2.98SFVYY420 pKa = 9.87AAHH423 pKa = 6.67IVHH426 pKa = 6.89TGALSQGDD434 pKa = 4.18PQDD437 pKa = 3.87AADD440 pKa = 4.47PADD443 pKa = 3.56PHH445 pKa = 6.13ATAEE449 pKa = 4.26LQFVTNFEE457 pKa = 4.27DD458 pKa = 5.38LEE460 pKa = 5.56DD461 pKa = 4.4YY462 pKa = 11.39DD463 pKa = 4.08LGAYY467 pKa = 8.29EE468 pKa = 4.4FLQVNIVTGHH478 pKa = 6.61GIDD481 pKa = 5.44LEE483 pKa = 4.29TDD485 pKa = 3.5PFNAVDD491 pKa = 5.01DD492 pKa = 4.34NMSLTKK498 pKa = 10.9YY499 pKa = 10.49GDD501 pKa = 3.51RR502 pKa = 11.84LSTFDD507 pKa = 4.94GNDD510 pKa = 3.09SVYY513 pKa = 11.06GGAGFDD519 pKa = 3.86TVDD522 pKa = 4.03GGDD525 pKa = 3.66GNDD528 pKa = 4.47FINGEE533 pKa = 4.03NHH535 pKa = 7.03ADD537 pKa = 3.68SLLGGAGDD545 pKa = 3.74DD546 pKa = 3.98TLIGGHH552 pKa = 6.4GVDD555 pKa = 3.7QLFGGVGHH563 pKa = 7.67DD564 pKa = 3.77SLDD567 pKa = 3.79AGSEE571 pKa = 3.91ADD573 pKa = 4.44RR574 pKa = 11.84VWGGDD579 pKa = 3.15GNDD582 pKa = 3.84IIRR585 pKa = 11.84AGSNFGEE592 pKa = 4.65SVDD595 pKa = 4.08GLWGEE600 pKa = 4.5AGNDD604 pKa = 3.57TLYY607 pKa = 11.5GDD609 pKa = 4.56AGFDD613 pKa = 3.7YY614 pKa = 11.11LSGGTGEE621 pKa = 4.78DD622 pKa = 3.62VLIGGDD628 pKa = 3.63QADD631 pKa = 4.3NLYY634 pKa = 11.02GDD636 pKa = 4.82AGNDD640 pKa = 3.32SLYY643 pKa = 11.02GGQGLDD649 pKa = 3.59RR650 pKa = 11.84MFGGDD655 pKa = 3.78GNDD658 pKa = 3.43YY659 pKa = 10.75LYY661 pKa = 10.68DD662 pKa = 3.49ITDD665 pKa = 3.42SNGFFGEE672 pKa = 4.58GGDD675 pKa = 4.19DD676 pKa = 3.64MMVAGNGNDD685 pKa = 3.08RR686 pKa = 11.84FFGGTGNDD694 pKa = 3.46IISAGGGNDD703 pKa = 3.67SIYY706 pKa = 11.07GGAGFDD712 pKa = 4.14TIIGGAGNDD721 pKa = 3.58VMRR724 pKa = 11.84GDD726 pKa = 4.49FNADD730 pKa = 3.06TFVFEE735 pKa = 5.61DD736 pKa = 3.52GHH738 pKa = 7.89GSDD741 pKa = 4.79TISDD745 pKa = 3.94FDD747 pKa = 4.33TNTNLEE753 pKa = 4.16QIDD756 pKa = 4.22LSGVSAIANVSDD768 pKa = 4.69LNLGSATSGAATQVGADD785 pKa = 3.45VVINTGGGNSIRR797 pKa = 11.84LTGVNIADD805 pKa = 4.41LEE807 pKa = 4.59AGDD810 pKa = 5.36FIFF813 pKa = 5.87

Molecular weight: 86.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A238JIC0|A0A238JIC0_9RHOB Rieske [2Fe-2S] domain protein OS=Tropicibacter phthalicicus OX=1037362 GN=TRP8649_04037 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.62GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.45AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4708

0

4708

1437188

29

3208

305.3

33.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.541 ± 0.045

0.952 ± 0.013

6.146 ± 0.039

6.121 ± 0.033

3.877 ± 0.021

8.462 ± 0.044

2.093 ± 0.02

5.221 ± 0.027

3.655 ± 0.028

10.038 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.801 ± 0.018

2.858 ± 0.019

4.894 ± 0.027

3.589 ± 0.02

6.053 ± 0.035

5.525 ± 0.029

5.404 ± 0.033

7.104 ± 0.03

1.412 ± 0.015

2.254 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski