Paracoccus liaowanqingii
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4493 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Z1BLE0|A0A4Z1BLE0_9RHOB Helix-turn-helix domain-containing protein OS=Paracoccus liaowanqingii OX=2560053 GN=E4L95_22845 PE=4 SV=1
MM1 pKa = 7.53 TLRR4 pKa = 11.84 TLTFSLAGTALAAGAAMAQDD24 pKa = 4.28 GEE26 pKa = 4.58 PIEE29 pKa = 5.1 LGIPVFLSGAASGPFGLPQQNGANVLIKK57 pKa = 10.47 ALNAGEE63 pKa = 4.45 VPAPYY68 pKa = 8.94 DD69 pKa = 3.36 TVGINGRR76 pKa = 11.84 MVEE79 pKa = 3.94 ATYY82 pKa = 11.17 VDD84 pKa = 4.06 EE85 pKa = 5.44 AADD88 pKa = 3.41 AVQEE92 pKa = 3.91 YY93 pKa = 10.41 RR94 pKa = 11.84 NLVEE98 pKa = 4.74 RR99 pKa = 11.84 DD100 pKa = 3.34 NVDD103 pKa = 2.87 AVVGYY108 pKa = 8.37 TSSGNCMAVAPLAEE122 pKa = 4.42 QFEE125 pKa = 4.61 QLTVLVDD132 pKa = 3.78 CGTPQVFEE140 pKa = 4.61 SVVTDD145 pKa = 3.29 PTYY148 pKa = 11.24 LFRR151 pKa = 11.84 TGPTGTIDD159 pKa = 3.07 SVGAVRR165 pKa = 11.84 YY166 pKa = 10.41 LMDD169 pKa = 3.76 TGVDD173 pKa = 3.41 MSRR176 pKa = 11.84 VGGINQNYY184 pKa = 10.15 AWGQDD189 pKa = 2.61 AWADD193 pKa = 3.76 FTGSLTALEE202 pKa = 4.31 TDD204 pKa = 3.69 SEE206 pKa = 4.65 IVTEE210 pKa = 3.9 QFPQVFAGQYY220 pKa = 8.12 GAEE223 pKa = 4.2 VV224 pKa = 3.27
Molecular weight: 23.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.745
IPC_protein 3.719
Toseland 3.516
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.617
Rodwell 3.554
Grimsley 3.427
Solomon 3.681
Lehninger 3.643
Nozaki 3.821
DTASelect 4.012
Thurlkill 3.567
EMBOSS 3.63
Sillero 3.834
Patrickios 0.947
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.747
Protein with the highest isoelectric point:
>tr|A0A4P7HP56|A0A4P7HP56_9RHOB Uncharacterized protein OS=Paracoccus liaowanqingii OX=2560053 GN=E4191_07915 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.12 GGRR28 pKa = 11.84 RR29 pKa = 11.84 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.69 GRR39 pKa = 11.84 KK40 pKa = 8.91 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4493
0
4493
1306414
27
1770
290.8
31.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.151 ± 0.048
0.813 ± 0.011
6.172 ± 0.036
5.192 ± 0.033
3.45 ± 0.022
8.891 ± 0.042
2.096 ± 0.017
5.058 ± 0.028
2.233 ± 0.033
10.512 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.782 ± 0.017
2.231 ± 0.019
5.557 ± 0.032
3.433 ± 0.021
7.499 ± 0.037
4.928 ± 0.024
5.44 ± 0.023
7.151 ± 0.032
1.439 ± 0.017
1.973 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here