Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces albidoflavus group; Streptomyces coelicolor

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8039 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P19255|GLPF_STRCO Probable glycerol uptake facilitator protein OS=Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) OX=100226 GN=glpF PE=2 SV=2
MM1 pKa = 7.63SVQQEE6 pKa = 4.04AAVDD10 pKa = 4.32GEE12 pKa = 4.3ALEE15 pKa = 4.23VWIDD19 pKa = 3.41QDD21 pKa = 3.99LCTGDD26 pKa = 5.01GICAQYY32 pKa = 10.9APEE35 pKa = 4.23VFEE38 pKa = 5.9LDD40 pKa = 3.05IDD42 pKa = 3.65GLAYY46 pKa = 10.7VKK48 pKa = 10.83GADD51 pKa = 4.87DD52 pKa = 4.98EE53 pKa = 5.3LLQAPGATTPVPLTLLTDD71 pKa = 3.78VVDD74 pKa = 4.21SAKK77 pKa = 10.15EE78 pKa = 3.93CPGEE82 pKa = 4.38CIHH85 pKa = 6.24VRR87 pKa = 11.84RR88 pKa = 11.84VSDD91 pKa = 3.16RR92 pKa = 11.84AEE94 pKa = 3.74IYY96 pKa = 11.01GPDD99 pKa = 3.59SEE101 pKa = 4.78

Molecular weight: 10.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P41108|Y4650_STRCO Putative lipoprotein SCO4650 OS=Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) OX=100226 GN=SCO4650 PE=3 SV=2
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILATRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.88GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8034

5

8039

2629592

19

7463

327.1

34.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.742 ± 0.043

0.779 ± 0.008

6.144 ± 0.026

5.696 ± 0.026

2.642 ± 0.015

9.622 ± 0.029

2.346 ± 0.014

2.868 ± 0.02

2.055 ± 0.022

10.219 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.698 ± 0.012

1.699 ± 0.014

6.203 ± 0.024

2.651 ± 0.017

8.362 ± 0.033

4.985 ± 0.019

6.178 ± 0.024

8.536 ± 0.027

1.521 ± 0.01

2.055 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski