Thogoto virus (isolate SiAr 126) (Tho)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Insthoviricetes; Articulavirales; Orthomyxoviridae; Thogotovirus; Thogoto thogotovirus

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9YNA4|PB2_THOGV Polymerase basic protein 2 OS=Thogoto virus (isolate SiAr 126) OX=126796 GN=Segment 1 PE=1 SV=1
MM1 pKa = 7.76ASNLPVRR8 pKa = 11.84SFSEE12 pKa = 4.51VCCAEE17 pKa = 3.5ARR19 pKa = 11.84AAIIQMEE26 pKa = 4.47NNPDD30 pKa = 3.21EE31 pKa = 4.62TVCNRR36 pKa = 11.84IWKK39 pKa = 8.82IHH41 pKa = 7.02RR42 pKa = 11.84DD43 pKa = 3.71LQSSDD48 pKa = 3.19LTTTVQVMMVYY59 pKa = 10.28RR60 pKa = 11.84FISKK64 pKa = 10.02RR65 pKa = 11.84VPEE68 pKa = 4.45GCFAILSGVNTGMYY82 pKa = 10.17NPRR85 pKa = 11.84EE86 pKa = 4.2LKK88 pKa = 10.28RR89 pKa = 11.84SYY91 pKa = 10.46VQSLSSGTSCEE102 pKa = 4.13FLRR105 pKa = 11.84SLDD108 pKa = 3.73KK109 pKa = 10.9LAKK112 pKa = 9.96NLLAVHH118 pKa = 6.15VCSDD122 pKa = 3.35VKK124 pKa = 10.67MSLNKK129 pKa = 10.09RR130 pKa = 11.84QVIDD134 pKa = 4.68FISGEE139 pKa = 4.18EE140 pKa = 4.31DD141 pKa = 3.34PTLHH145 pKa = 5.9TAEE148 pKa = 5.08HH149 pKa = 6.01LTSLALDD156 pKa = 4.42DD157 pKa = 4.56SPSAVVYY164 pKa = 10.53SGWQQEE170 pKa = 4.25AIKK173 pKa = 10.42LHH175 pKa = 4.32NTIRR179 pKa = 11.84KK180 pKa = 7.43IATMRR185 pKa = 11.84PADD188 pKa = 3.75CKK190 pKa = 10.8AGKK193 pKa = 9.65FYY195 pKa = 11.29SDD197 pKa = 3.78ILSACDD203 pKa = 3.26QTKK206 pKa = 10.49EE207 pKa = 4.09LLDD210 pKa = 4.42AFDD213 pKa = 3.87QGKK216 pKa = 8.86LAYY219 pKa = 10.17DD220 pKa = 3.57RR221 pKa = 11.84DD222 pKa = 3.94VVLIGWMDD230 pKa = 4.31EE231 pKa = 4.01IIKK234 pKa = 10.3IFSKK238 pKa = 10.52PDD240 pKa = 3.14YY241 pKa = 10.71LEE243 pKa = 4.46AKK245 pKa = 8.94GVSYY249 pKa = 10.55QVLKK253 pKa = 11.03NVSNKK258 pKa = 9.26VALLRR263 pKa = 11.84EE264 pKa = 4.53SIWWVTEE271 pKa = 3.56LDD273 pKa = 3.28GRR275 pKa = 11.84EE276 pKa = 3.77YY277 pKa = 11.39LFFDD281 pKa = 4.02EE282 pKa = 4.35SWYY285 pKa = 10.1LHH287 pKa = 5.71GMSAFSDD294 pKa = 4.06GVPGYY299 pKa = 10.91EE300 pKa = 4.2DD301 pKa = 4.82FIYY304 pKa = 10.86

Molecular weight: 34.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q77D96|M1_THOGV Matrix protein OS=Thogoto virus (isolate SiAr 126) OX=126796 GN=Segment 6 PE=4 SV=1
MM1 pKa = 7.45ATDD4 pKa = 3.57QMDD7 pKa = 3.23ISGPPPKK14 pKa = 9.55KK15 pKa = 9.6QHH17 pKa = 6.12VDD19 pKa = 3.28TEE21 pKa = 4.41SQIPKK26 pKa = 8.4MYY28 pKa = 10.93EE29 pKa = 3.7MIRR32 pKa = 11.84DD33 pKa = 3.62QMRR36 pKa = 11.84TLASTHH42 pKa = 6.68KK43 pKa = 10.06IPLNIDD49 pKa = 3.42HH50 pKa = 6.66NCEE53 pKa = 4.18VIGSIIMAACTNNRR67 pKa = 11.84DD68 pKa = 3.52LRR70 pKa = 11.84PVDD73 pKa = 4.09KK74 pKa = 10.73YY75 pKa = 10.27WFLMGPAGAEE85 pKa = 3.79VMTEE89 pKa = 3.97VEE91 pKa = 5.28IDD93 pKa = 3.61IQPQLQWAKK102 pKa = 10.8GAVHH106 pKa = 6.7DD107 pKa = 4.83PKK109 pKa = 11.4YY110 pKa = 10.38KK111 pKa = 9.24GQWYY115 pKa = 8.51PFLALLQISNKK126 pKa = 9.26TKK128 pKa = 10.05DD129 pKa = 3.15TILWQKK135 pKa = 10.74YY136 pKa = 8.83PVTQEE141 pKa = 4.54LEE143 pKa = 3.83ISNSLEE149 pKa = 3.7IYY151 pKa = 11.01ANGHH155 pKa = 5.62GIKK158 pKa = 10.29DD159 pKa = 3.4RR160 pKa = 11.84LKK162 pKa = 9.09NSRR165 pKa = 11.84PRR167 pKa = 11.84SVGPLVHH174 pKa = 6.94LLHH177 pKa = 7.33LKK179 pKa = 10.17RR180 pKa = 11.84LQEE183 pKa = 4.25NPPKK187 pKa = 10.56NPKK190 pKa = 8.08TKK192 pKa = 10.5KK193 pKa = 9.27PLEE196 pKa = 4.39SPAVNGIRR204 pKa = 11.84KK205 pKa = 9.87SIVGHH210 pKa = 6.69LKK212 pKa = 9.68RR213 pKa = 11.84QCIGEE218 pKa = 4.24TQKK221 pKa = 11.76AMINQFEE228 pKa = 4.26MGRR231 pKa = 11.84WEE233 pKa = 4.22SLSTFAASLLAIKK246 pKa = 9.97PRR248 pKa = 11.84IEE250 pKa = 3.67NHH252 pKa = 5.91FVLTYY257 pKa = 9.88PLIANCEE264 pKa = 4.09DD265 pKa = 3.52FAGATLSDD273 pKa = 3.26EE274 pKa = 4.49WVFKK278 pKa = 11.14AMEE281 pKa = 4.86KK282 pKa = 10.17ISNKK286 pKa = 8.35KK287 pKa = 6.11TLRR290 pKa = 11.84VCGPDD295 pKa = 3.41EE296 pKa = 4.04KK297 pKa = 10.51WISFMNQIYY306 pKa = 9.56IHH308 pKa = 6.43SVFQTTGEE316 pKa = 4.02DD317 pKa = 3.25LGVLEE322 pKa = 4.73WVFGGRR328 pKa = 11.84FCQRR332 pKa = 11.84KK333 pKa = 8.8EE334 pKa = 3.73FGRR337 pKa = 11.84YY338 pKa = 8.32CKK340 pKa = 10.28KK341 pKa = 10.66SQTKK345 pKa = 10.48VIGLFTFQYY354 pKa = 9.89EE355 pKa = 4.54YY356 pKa = 10.22WSKK359 pKa = 10.31PLKK362 pKa = 9.97SAPRR366 pKa = 11.84SIEE369 pKa = 3.64GSKK372 pKa = 10.15RR373 pKa = 11.84GQISCRR379 pKa = 11.84PSFKK383 pKa = 10.57GKK385 pKa = 10.08RR386 pKa = 11.84PSYY389 pKa = 11.51NNFTSIDD396 pKa = 3.74ALQSASGSQTVSFYY410 pKa = 11.06DD411 pKa = 3.41QVRR414 pKa = 11.84EE415 pKa = 4.0EE416 pKa = 4.22CQKK419 pKa = 11.36YY420 pKa = 9.02MDD422 pKa = 4.74LKK424 pKa = 11.47VEE426 pKa = 4.2GTTCFYY432 pKa = 10.97RR433 pKa = 11.84KK434 pKa = 9.61GGHH437 pKa = 5.66VEE439 pKa = 4.01VEE441 pKa = 4.64FPGSAHH447 pKa = 6.57CNTYY451 pKa = 11.03LFGG454 pKa = 5.58

Molecular weight: 51.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

3637

266

769

519.6

59.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.801 ± 0.364

2.337 ± 0.236

5.334 ± 0.405

7.231 ± 0.552

3.932 ± 0.336

5.307 ± 0.367

2.392 ± 0.098

5.829 ± 0.35

6.571 ± 0.445

9.211 ± 0.486

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.64 ± 0.339

3.987 ± 0.269

4.839 ± 0.415

3.822 ± 0.368

5.856 ± 0.432

7.451 ± 0.519

5.884 ± 0.372

6.379 ± 0.371

1.705 ± 0.327

3.492 ± 0.207
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski