Brevibacillus panacihumi W25

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Brevibacillus; Brevibacillus panacihumi

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5188 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V6M4R0|V6M4R0_9BACL Spore germination protein OS=Brevibacillus panacihumi W25 OX=1408254 GN=T458_22635 PE=3 SV=1
MM1 pKa = 7.27KK2 pKa = 10.52ALVEE6 pKa = 4.81FCASNVASYY15 pKa = 8.43TQAVMDD21 pKa = 5.16ALEE24 pKa = 4.37QDD26 pKa = 3.76PEE28 pKa = 4.65LDD30 pKa = 3.43VDD32 pKa = 3.95VLEE35 pKa = 4.5YY36 pKa = 11.28GCLGYY41 pKa = 10.76CGEE44 pKa = 5.14CYY46 pKa = 10.62LEE48 pKa = 4.78PFALVNGKK56 pKa = 8.92LVQAPTAEE64 pKa = 4.13ALLDD68 pKa = 4.26KK69 pKa = 10.56IKK71 pKa = 10.74QVLKK75 pKa = 11.07EE76 pKa = 3.97EE77 pKa = 4.9DD78 pKa = 4.59DD79 pKa = 4.0PFKK82 pKa = 10.96DD83 pKa = 4.34LPLL86 pKa = 5.41

Molecular weight: 9.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V6MAB5|V6MAB5_9BACL Membrane protein OS=Brevibacillus panacihumi W25 OX=1408254 GN=T458_07100 PE=3 SV=1
MM1 pKa = 7.75SYY3 pKa = 11.08LPTVVQAIEE12 pKa = 4.18PKK14 pKa = 10.43SEE16 pKa = 3.67VDD18 pKa = 3.72LYY20 pKa = 11.31LPQAHH25 pKa = 5.02MRR27 pKa = 11.84KK28 pKa = 9.13WNLQPSTLTVQFGNKK43 pKa = 6.61TVRR46 pKa = 11.84ARR48 pKa = 11.84VSRR51 pKa = 11.84MEE53 pKa = 3.98RR54 pKa = 11.84KK55 pKa = 8.88SRR57 pKa = 11.84IWIRR61 pKa = 11.84TSMAQSLHH69 pKa = 6.57LPTGVPLLVDD79 pKa = 3.35YY80 pKa = 10.36QANQQSLVFGPYY92 pKa = 9.31MGILVSAYY100 pKa = 9.79NLQYY104 pKa = 10.66PLAPFGPLSHH114 pKa = 7.29FFNEE118 pKa = 4.44VADD121 pKa = 4.06SCRR124 pKa = 11.84KK125 pKa = 9.5RR126 pKa = 11.84GGVICAFRR134 pKa = 11.84PQDD137 pKa = 3.79VNWDD141 pKa = 3.39AGIVRR146 pKa = 11.84GLVRR150 pKa = 11.84KK151 pKa = 9.88GGSWRR156 pKa = 11.84QQVLPLPQCIYY167 pKa = 10.31NRR169 pKa = 11.84LVSRR173 pKa = 11.84QRR175 pKa = 11.84EE176 pKa = 3.79RR177 pKa = 11.84SEE179 pKa = 5.3AMSAWVQRR187 pKa = 11.84CKK189 pKa = 10.38EE190 pKa = 3.84ANIPFFNEE198 pKa = 3.18RR199 pKa = 11.84FLNKK203 pKa = 8.65WHH205 pKa = 6.0VHH207 pKa = 4.35TALEE211 pKa = 4.21KK212 pKa = 10.38QEE214 pKa = 4.3AAVPHH219 pKa = 6.14LPGMLRR225 pKa = 11.84YY226 pKa = 9.43EE227 pKa = 4.31NQDD230 pKa = 3.65DD231 pKa = 4.22LQEE234 pKa = 4.46MITRR238 pKa = 11.84YY239 pKa = 7.86HH240 pKa = 5.27TVYY243 pKa = 10.79AKK245 pKa = 10.03PANGSMGRR253 pKa = 11.84GIIRR257 pKa = 11.84LRR259 pKa = 11.84RR260 pKa = 11.84KK261 pKa = 9.49QNGYY265 pKa = 9.02QAASPGGLNKK275 pKa = 10.08HH276 pKa = 6.26FSSISGLHH284 pKa = 6.21KK285 pKa = 10.62YY286 pKa = 9.92LSKK289 pKa = 9.88RR290 pKa = 11.84TKK292 pKa = 9.95GKK294 pKa = 9.78PYY296 pKa = 10.54LLQQGLPLIGVQNRR310 pKa = 11.84PADD313 pKa = 3.78FRR315 pKa = 11.84VLVQKK320 pKa = 10.33DD321 pKa = 3.72RR322 pKa = 11.84KK323 pKa = 9.83GEE325 pKa = 3.75WSITSLVARR334 pKa = 11.84LGQNRR339 pKa = 11.84IVSNVSRR346 pKa = 11.84GGSMMSPAHH355 pKa = 6.95ALRR358 pKa = 11.84LCGPWACSQRR368 pKa = 11.84PTPQTLKK375 pKa = 10.74HH376 pKa = 5.52VALKK380 pKa = 10.6LSRR383 pKa = 11.84LLEE386 pKa = 4.17EE387 pKa = 4.94ALPGHH392 pKa = 5.63YY393 pKa = 10.59AEE395 pKa = 5.36FGVDD399 pKa = 3.17LGVDD403 pKa = 3.26VRR405 pKa = 11.84GQVWLLEE412 pKa = 4.49VNSKK416 pKa = 10.07PSKK419 pKa = 8.71TVNSIPIPEE428 pKa = 4.38GAEE431 pKa = 3.81APPRR435 pKa = 11.84RR436 pKa = 11.84ARR438 pKa = 11.84PSAVRR443 pKa = 11.84MFEE446 pKa = 3.93YY447 pKa = 10.61ASYY450 pKa = 10.85ISGFPRR456 pKa = 11.84PSPNRR461 pKa = 11.84KK462 pKa = 5.93TARR465 pKa = 11.84KK466 pKa = 6.76KK467 pKa = 7.74TRR469 pKa = 11.84RR470 pKa = 11.84RR471 pKa = 3.38

Molecular weight: 53.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5188

0

5188

1544730

27

2184

297.8

33.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.254 ± 0.039

0.792 ± 0.01

4.88 ± 0.024

7.212 ± 0.041

4.064 ± 0.027

7.202 ± 0.033

2.203 ± 0.018

6.638 ± 0.035

5.483 ± 0.031

10.229 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.913 ± 0.02

3.384 ± 0.022

4.167 ± 0.024

4.32 ± 0.029

5.243 ± 0.032

5.863 ± 0.025

5.306 ± 0.024

7.394 ± 0.028

1.223 ± 0.013

3.229 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski