Loktanella sp. 5RATIMAR09
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3530 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0Q2UTY9|A0A0Q2UTY9_9RHOB Uncharacterized protein OS=Loktanella sp. 5RATIMAR09 OX=1225655 GN=AN191_04095 PE=4 SV=1
MM1 pKa = 7.43 TMTSKK6 pKa = 10.64 FARR9 pKa = 11.84 TSMLAAPLAIVAGMATAGGLAEE31 pKa = 4.38 PVEE34 pKa = 4.75 TPAPVVVAPAPVMMGNDD51 pKa = 2.87 WTGFYY56 pKa = 10.69 AGGQLGYY63 pKa = 11.04 GRR65 pKa = 11.84 LDD67 pKa = 3.44 PSVDD71 pKa = 3.62 TDD73 pKa = 3.58 PSEE76 pKa = 4.4 PDD78 pKa = 3.14 GVIYY82 pKa = 10.03 GVHH85 pKa = 6.51 AGYY88 pKa = 10.77 NYY90 pKa = 10.93 DD91 pKa = 3.45 FGSVVLGGEE100 pKa = 3.66 IDD102 pKa = 3.8 YY103 pKa = 11.2 DD104 pKa = 3.67 LTDD107 pKa = 3.77 ISFDD111 pKa = 3.97 TPDD114 pKa = 3.35 SDD116 pKa = 4.7 LDD118 pKa = 3.66 GVLRR122 pKa = 11.84 AKK124 pKa = 10.77 AKK126 pKa = 10.41 LGYY129 pKa = 9.93 DD130 pKa = 3.32 AGAFMPYY137 pKa = 8.25 ITAGYY142 pKa = 9.5 AQAQTSDD149 pKa = 4.69 DD150 pKa = 4.7 LDD152 pKa = 5.98 DD153 pKa = 4.54 DD154 pKa = 4.09 TDD156 pKa = 3.79 GAFAGLGMSYY166 pKa = 10.26 MMSDD170 pKa = 3.34 SLILGGEE177 pKa = 4.3 VLQHH181 pKa = 5.4 QFEE184 pKa = 4.75 DD185 pKa = 3.73 VADD188 pKa = 4.23 AADD191 pKa = 3.59 VDD193 pKa = 4.2 ATTLSLRR200 pKa = 11.84 ASFKK204 pKa = 10.72 FF205 pKa = 3.49
Molecular weight: 21.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.692
IPC2_protein 3.605
IPC_protein 3.643
Toseland 3.401
ProMoST 3.834
Dawson 3.656
Bjellqvist 3.821
Wikipedia 3.643
Rodwell 3.465
Grimsley 3.312
Solomon 3.643
Lehninger 3.605
Nozaki 3.783
DTASelect 4.088
Thurlkill 3.478
EMBOSS 3.643
Sillero 3.77
Patrickios 1.036
IPC_peptide 3.643
IPC2_peptide 3.745
IPC2.peptide.svr19 3.717
Protein with the highest isoelectric point:
>tr|A0A0Q2UQL1|A0A0Q2UQL1_9RHOB Phosphatidate cytidylyltransferase OS=Loktanella sp. 5RATIMAR09 OX=1225655 GN=AN191_06015 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 VRR12 pKa = 11.84 KK13 pKa = 8.99 NRR15 pKa = 11.84 HH16 pKa = 3.77 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.53 ILNNRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.47 GRR39 pKa = 11.84 AKK41 pKa = 10.69 LSAA44 pKa = 3.92
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3530
0
3530
1075750
36
2146
304.7
33.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.366 ± 0.056
0.891 ± 0.014
6.231 ± 0.04
5.53 ± 0.04
3.864 ± 0.027
8.475 ± 0.042
2.009 ± 0.023
5.561 ± 0.032
3.289 ± 0.037
9.824 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.913 ± 0.021
2.786 ± 0.024
4.888 ± 0.029
3.341 ± 0.023
6.164 ± 0.038
5.001 ± 0.028
5.769 ± 0.028
7.42 ± 0.036
1.399 ± 0.018
2.279 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here