Pepper leaf curl virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9PY61|Q9PY61_9GEMI Transcriptional activator protein OS=Pepper leaf curl virus OX=83839 PE=3 SV=1
MM1 pKa = 7.78APPKK5 pKa = 10.22NLRR8 pKa = 11.84INAKK12 pKa = 10.13NYY14 pKa = 8.98FLTYY18 pKa = 7.85PHH20 pKa = 7.15CSLTKK25 pKa = 10.57EE26 pKa = 4.09EE27 pKa = 4.94ALSQIQAIEE36 pKa = 4.11TPTIKK41 pKa = 10.59LFIRR45 pKa = 11.84ICRR48 pKa = 11.84EE49 pKa = 3.5LHH51 pKa = 6.58EE52 pKa = 6.12DD53 pKa = 3.78GTPHH57 pKa = 6.98LHH59 pKa = 7.02ILIQFEE65 pKa = 5.32GKK67 pKa = 9.19FQCKK71 pKa = 8.84NPRR74 pKa = 11.84FFDD77 pKa = 3.39LTSPTRR83 pKa = 11.84SAHH86 pKa = 4.94FHH88 pKa = 6.55PNIQGAKK95 pKa = 9.57SSTDD99 pKa = 3.15VKK101 pKa = 11.02EE102 pKa = 4.43YY103 pKa = 9.84IAKK106 pKa = 10.64NGDD109 pKa = 3.71VLDD112 pKa = 4.51HH113 pKa = 6.92GVFQIDD119 pKa = 3.19GRR121 pKa = 11.84SARR124 pKa = 11.84GGCQSANDD132 pKa = 4.21AYY134 pKa = 10.87AEE136 pKa = 4.15ALNSGTKK143 pKa = 10.29ASALAILKK151 pKa = 10.3EE152 pKa = 4.25EE153 pKa = 4.15APKK156 pKa = 10.61DD157 pKa = 3.8YY158 pKa = 10.69ILQFHH163 pKa = 6.68NLNSNLDD170 pKa = 3.8KK171 pKa = 10.95IFSLRR176 pKa = 11.84IEE178 pKa = 4.31MYY180 pKa = 9.74ISPFLVSFDD189 pKa = 3.34QVQRR193 pKa = 11.84NLNEE197 pKa = 3.52WVAANVVKK205 pKa = 9.85PARR208 pKa = 11.84PLRR211 pKa = 11.84PISIVVEE218 pKa = 4.09GDD220 pKa = 3.03SRR222 pKa = 11.84TGKK225 pKa = 8.52TMWARR230 pKa = 11.84SLGPHH235 pKa = 6.71NYY237 pKa = 10.18LCGHH241 pKa = 7.38LDD243 pKa = 4.39LSPKK247 pKa = 10.13VYY249 pKa = 11.08NNDD252 pKa = 2.39PWYY255 pKa = 11.17NVIDD259 pKa = 4.62DD260 pKa = 4.37VDD262 pKa = 3.61PHH264 pKa = 5.85YY265 pKa = 11.0LKK267 pKa = 10.7HH268 pKa = 6.2FKK270 pKa = 10.7EE271 pKa = 4.46FMGAQRR277 pKa = 11.84DD278 pKa = 3.81WQSNTKK284 pKa = 9.6YY285 pKa = 10.57GKK287 pKa = 9.12PVQIKK292 pKa = 10.41GGIPTIFLCNPGPHH306 pKa = 6.2SSYY309 pKa = 11.35KK310 pKa = 10.55EE311 pKa = 3.69FLEE314 pKa = 4.31EE315 pKa = 4.4EE316 pKa = 4.39KK317 pKa = 10.29NTALKK322 pKa = 10.55NWALKK327 pKa = 10.23NAIFVTIEE335 pKa = 4.02GPLFSGSHH343 pKa = 5.23QGATQNRR350 pKa = 11.84QEE352 pKa = 4.42DD353 pKa = 3.7NN354 pKa = 3.52

Molecular weight: 40.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9PY64|Q9PY64_9GEMI Protein V2 OS=Pepper leaf curl virus OX=83839 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.52IVISTPASKK16 pKa = 10.29VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.04SPYY27 pKa = 9.79VSRR30 pKa = 11.84AAAPTVLVTNKK41 pKa = 9.33RR42 pKa = 11.84RR43 pKa = 11.84SWANRR48 pKa = 11.84TMYY51 pKa = 10.17RR52 pKa = 11.84KK53 pKa = 9.02PRR55 pKa = 11.84IYY57 pKa = 10.78RR58 pKa = 11.84MYY60 pKa = 10.47KK61 pKa = 10.02SPDD64 pKa = 3.18VPRR67 pKa = 11.84GCEE70 pKa = 4.34GPCKK74 pKa = 9.32VQSYY78 pKa = 6.95EE79 pKa = 3.75QRR81 pKa = 11.84HH82 pKa = 5.6DD83 pKa = 3.46VAHH86 pKa = 5.81VGKK89 pKa = 9.73VICVSDD95 pKa = 3.47VTRR98 pKa = 11.84GNGLTHH104 pKa = 7.15RR105 pKa = 11.84VGKK108 pKa = 10.63GPVIKK113 pKa = 10.24SVYY116 pKa = 7.53VWGRR120 pKa = 11.84IWMDD124 pKa = 3.98EE125 pKa = 3.75ISSQEE130 pKa = 3.74SHH132 pKa = 5.71QSVMFFLVRR141 pKa = 11.84DD142 pKa = 3.79RR143 pKa = 11.84RR144 pKa = 11.84PFGTPQDD151 pKa = 3.81FGQVFNMYY159 pKa = 10.66DD160 pKa = 3.86NEE162 pKa = 4.34PSTATVKK169 pKa = 10.73NDD171 pKa = 2.82NRR173 pKa = 11.84DD174 pKa = 3.13RR175 pKa = 11.84FQVLRR180 pKa = 11.84RR181 pKa = 11.84FQATVTGGQYY191 pKa = 10.83ASKK194 pKa = 9.78EE195 pKa = 3.78QAIVRR200 pKa = 11.84KK201 pKa = 9.26FMKK204 pKa = 10.33VNNHH208 pKa = 3.97VTYY211 pKa = 10.83NHH213 pKa = 6.01QEE215 pKa = 3.45AAKK218 pKa = 10.14YY219 pKa = 10.5DD220 pKa = 3.75NHH222 pKa = 6.5TEE224 pKa = 4.02NALLLYY230 pKa = 7.29MACTHH235 pKa = 7.07ASNPVYY241 pKa = 9.86ATLKK245 pKa = 9.47IRR247 pKa = 11.84IYY249 pKa = 10.65FYY251 pKa = 11.33DD252 pKa = 3.43SVQNN256 pKa = 3.87

Molecular weight: 29.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1084

97

354

180.7

20.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.996 ± 0.453

2.214 ± 0.45

4.428 ± 0.415

4.336 ± 0.545

4.244 ± 0.39

4.982 ± 0.378

4.52 ± 0.444

6.181 ± 0.958

5.535 ± 0.586

7.103 ± 1.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.214 ± 0.494

5.72 ± 0.688

5.812 ± 0.678

5.627 ± 0.563

7.103 ± 1.152

7.934 ± 1.437

5.258 ± 0.479

5.996 ± 1.314

1.015 ± 0.186

3.782 ± 0.654
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski