Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus)

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae; Saccharolobus; Saccharolobus solfataricus

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2938 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q97U05|Q97U05_SACS2 Amino acid transporter related protein OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=SSO3224 PE=4 SV=1
MM1 pKa = 7.03EE2 pKa = 5.07TLNEE6 pKa = 4.33SKK8 pKa = 10.83KK9 pKa = 10.5EE10 pKa = 3.86FYY12 pKa = 10.17TYY14 pKa = 10.64FISTSKK20 pKa = 10.56FYY22 pKa = 11.31YY23 pKa = 10.39DD24 pKa = 4.74LSNTVNSPIVVCEE37 pKa = 3.85MLYY40 pKa = 10.41EE41 pKa = 4.89AINAGIKK48 pKa = 9.97LLSYY52 pKa = 10.52YY53 pKa = 10.61FSLQDD58 pKa = 3.23KK59 pKa = 10.1PRR61 pKa = 11.84TEE63 pKa = 4.19VVKK66 pKa = 10.29EE67 pKa = 3.8LSSILGDD74 pKa = 3.11WVEE77 pKa = 4.5YY78 pKa = 9.71YY79 pKa = 10.07WNLGLTLHH87 pKa = 6.24YY88 pKa = 10.73DD89 pKa = 3.99CYY91 pKa = 11.34LSGNVDD97 pKa = 3.37EE98 pKa = 6.27DD99 pKa = 5.64DD100 pKa = 3.61IPLYY104 pKa = 10.45EE105 pKa = 4.19NQVKK109 pKa = 10.64DD110 pKa = 4.47FISRR114 pKa = 11.84VEE116 pKa = 3.96EE117 pKa = 4.25VVFGG121 pKa = 3.88

Molecular weight: 14.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q97YK4|Q97YK4_SACS2 Second ORF in transposon ISC1316 OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=SSO7675 PE=4 SV=1
MM1 pKa = 6.95HH2 pKa = 6.96QSTRR6 pKa = 11.84RR7 pKa = 11.84GRR9 pKa = 11.84EE10 pKa = 3.58LMRR13 pKa = 11.84KK14 pKa = 8.29YY15 pKa = 10.44SHH17 pKa = 7.0RR18 pKa = 11.84EE19 pKa = 3.37RR20 pKa = 11.84NRR22 pKa = 11.84VLDD25 pKa = 4.36FVHH28 pKa = 6.84KK29 pKa = 10.11FVNTLLDD36 pKa = 4.62LYY38 pKa = 10.18PMTFFAVEE46 pKa = 4.13KK47 pKa = 10.36LNKK50 pKa = 10.05EE51 pKa = 4.6SMFKK55 pKa = 10.61DD56 pKa = 3.43ANDD59 pKa = 3.51SLSRR63 pKa = 11.84KK64 pKa = 9.09ISRR67 pKa = 11.84TVWRR71 pKa = 11.84SIHH74 pKa = 6.78RR75 pKa = 11.84VLKK78 pKa = 10.63YY79 pKa = 9.94KK80 pKa = 10.85APLYY84 pKa = 10.4GSFVKK89 pKa = 10.31EE90 pKa = 3.71VNPYY94 pKa = 8.3LTSRR98 pKa = 11.84SCPRR102 pKa = 11.84CRR104 pKa = 11.84FVSRR108 pKa = 11.84KK109 pKa = 8.54VGKK112 pKa = 7.94TFEE115 pKa = 4.21CEE117 pKa = 3.44RR118 pKa = 11.84CGFKK122 pKa = 10.55LDD124 pKa = 3.66RR125 pKa = 11.84QLNASLNIYY134 pKa = 10.63LKK136 pKa = 9.76MCGFPHH142 pKa = 6.88IRR144 pKa = 11.84EE145 pKa = 3.97IARR148 pKa = 11.84VWVGVIPLMGRR159 pKa = 11.84RR160 pKa = 11.84RR161 pKa = 11.84MNVRR165 pKa = 11.84DD166 pKa = 3.95FGEE169 pKa = 4.36AQGLRR174 pKa = 11.84VDD176 pKa = 4.17IKK178 pKa = 10.44YY179 pKa = 10.59HH180 pKa = 6.42EE181 pKa = 4.48IPP183 pKa = 4.03

Molecular weight: 21.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2938

0

2938

827883

40

1426

281.8

31.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.621 ± 0.05

0.618 ± 0.015

4.694 ± 0.038

6.807 ± 0.054

4.434 ± 0.036

6.44 ± 0.038

1.294 ± 0.016

9.5 ± 0.054

7.692 ± 0.061

10.356 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.201 ± 0.018

4.977 ± 0.041

3.814 ± 0.032

2.102 ± 0.025

4.684 ± 0.043

6.716 ± 0.041

4.734 ± 0.039

7.448 ± 0.035

1.049 ± 0.018

4.82 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski