Dendrolimus punctatus cypovirus 22
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 16 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A7E6B9|A0A0A7E6B9_9REOV Uncharacterized protein OS=Dendrolimus punctatus cypovirus 22 OX=1577776 PE=4 SV=1
MM1 pKa = 8.15 EE2 pKa = 4.61 YY3 pKa = 9.76 PQSGFFAAEE12 pKa = 4.01 TPNDD16 pKa = 3.2 NRR18 pKa = 11.84 TITLLQVRR26 pKa = 11.84 GQQPRR31 pKa = 11.84 NIPITDD37 pKa = 3.43 ILDD40 pKa = 4.31 HH41 pKa = 7.46 IEE43 pKa = 4.51 GPNTDD48 pKa = 4.3 EE49 pKa = 4.75 LFEE52 pKa = 4.39 IQDD55 pKa = 3.55 VTEE58 pKa = 4.19 YY59 pKa = 10.95 EE60 pKa = 4.24 PDD62 pKa = 3.47 HH63 pKa = 6.01 TTHH66 pKa = 6.5 ATNTQVFNINVLLRR80 pKa = 11.84 SVFLSEE86 pKa = 4.12 MTNVSQIPHH95 pKa = 6.85 RR96 pKa = 11.84 FGIFNVMSAEE106 pKa = 4.15 DD107 pKa = 3.86 ATCVVQKK114 pKa = 10.84 DD115 pKa = 3.62 GTYY118 pKa = 9.01 YY119 pKa = 11.21 ASGKK123 pKa = 7.85 TRR125 pKa = 11.84 DD126 pKa = 3.83 ADD128 pKa = 4.32 SIPHH132 pKa = 5.1 VYY134 pKa = 9.61 IRR136 pKa = 11.84 NFALCMLKK144 pKa = 10.11 VAPYY148 pKa = 10.68 LRR150 pKa = 11.84 GDD152 pKa = 4.51 RR153 pKa = 11.84 INVFAQLTHH162 pKa = 6.56 FMQTVRR168 pKa = 11.84 RR169 pKa = 11.84 NRR171 pKa = 11.84 SKK173 pKa = 10.25 FWLRR177 pKa = 11.84 FNLSDD182 pKa = 5.15 LNPLHH187 pKa = 7.12 LSNQLSKK194 pKa = 11.29 FNLFLLLLDD203 pKa = 4.32 LANYY207 pKa = 8.56 EE208 pKa = 4.23 HH209 pKa = 6.57 FQVNQSAMLLGSINACLVCLFAAGISDD236 pKa = 3.4 AVEE239 pKa = 3.63 RR240 pKa = 11.84 TTIDD244 pKa = 3.2 EE245 pKa = 4.24 VKK247 pKa = 10.28 YY248 pKa = 10.24 LQFKK252 pKa = 10.11 IPSPSEE258 pKa = 3.59 NYY260 pKa = 9.08 VRR262 pKa = 11.84 KK263 pKa = 10.12 DD264 pKa = 3.04 LLQPFFHH271 pKa = 6.37 SQRR274 pKa = 11.84 AIFVGFLINAFIQPYY289 pKa = 9.01 MIVAFPLEE297 pKa = 4.08 VRR299 pKa = 11.84 NQLFPSNRR307 pKa = 11.84 VPVVRR312 pKa = 11.84 EE313 pKa = 3.98 DD314 pKa = 4.03 EE315 pKa = 4.34 PVSDD319 pKa = 4.41 HH320 pKa = 8.04 DD321 pKa = 4.88 SGEE324 pKa = 3.82 ASNFYY329 pKa = 10.91 DD330 pKa = 5.06 LYY332 pKa = 11.44 VSLNYY337 pKa = 10.46 GAFPSDD343 pKa = 2.83 GGIYY347 pKa = 10.05 KK348 pKa = 10.32 YY349 pKa = 10.73 LSNSDD354 pKa = 3.5 DD355 pKa = 3.91 VIYY358 pKa = 11.06 LDD360 pKa = 5.15 VFDD363 pKa = 5.03 TMPFQQRR370 pKa = 11.84 YY371 pKa = 4.96 QKK373 pKa = 10.95 RR374 pKa = 11.84 NIRR377 pKa = 11.84 GLFDD381 pKa = 3.6 DD382 pKa = 4.64 TLNTNLNVIRR392 pKa = 11.84 VVHH395 pKa = 6.33 HH396 pKa = 6.69 EE397 pKa = 4.85 DD398 pKa = 3.44 YY399 pKa = 11.42 DD400 pKa = 4.22 SVLLLDD406 pKa = 4.12 ILQPFNEE413 pKa = 4.28 SLSRR417 pKa = 11.84 CLLTRR422 pKa = 11.84 YY423 pKa = 10.01 IIDD426 pKa = 3.19 RR427 pKa = 11.84 RR428 pKa = 11.84 MIAVIASSTPCGLSDD443 pKa = 3.76 GQLIFNAHH451 pKa = 4.54 QLEE454 pKa = 4.45 VFEE457 pKa = 6.01 LITEE461 pKa = 4.64 RR462 pKa = 11.84 YY463 pKa = 7.68 MNDD466 pKa = 3.62 VIRR469 pKa = 11.84 HH470 pKa = 5.39 IPDD473 pKa = 3.94 GYY475 pKa = 11.45 GLAEE479 pKa = 4.18 DD480 pKa = 4.14 RR481 pKa = 11.84 ARR483 pKa = 11.84 VLHH486 pKa = 6.83 EE487 pKa = 3.95 ILALQASYY495 pKa = 11.06 INEE498 pKa = 3.54 IKK500 pKa = 8.11 MTYY503 pKa = 10.39 KK504 pKa = 10.7 DD505 pKa = 3.48 FSEE508 pKa = 4.75 AYY510 pKa = 8.7 KK511 pKa = 10.7 HH512 pKa = 5.01 KK513 pKa = 9.39 TRR515 pKa = 11.84 SNVSLEE521 pKa = 4.06 KK522 pKa = 10.13 RR523 pKa = 11.84 WNEE526 pKa = 3.22 AGIRR530 pKa = 11.84 EE531 pKa = 4.32 FVTCGLMLL539 pKa = 4.57
Molecular weight: 62.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.265
IPC2_protein 5.27
IPC_protein 5.27
Toseland 5.474
ProMoST 5.525
Dawson 5.397
Bjellqvist 5.474
Wikipedia 5.347
Rodwell 5.347
Grimsley 5.537
Solomon 5.397
Lehninger 5.372
Nozaki 5.601
DTASelect 5.779
Thurlkill 5.601
EMBOSS 5.575
Sillero 5.677
Patrickios 3.808
IPC_peptide 5.41
IPC2_peptide 5.69
IPC2.peptide.svr19 5.594
Protein with the highest isoelectric point:
>tr|A0A0A7E7V5|A0A0A7E7V5_9REOV Uncharacterized protein OS=Dendrolimus punctatus cypovirus 22 OX=1577776 PE=4 SV=1
MM1 pKa = 7.47 KK2 pKa = 9.79 KK3 pKa = 10.12 KK4 pKa = 10.71 SPASPLEE11 pKa = 4.16 TLQSTMLDD19 pKa = 3.58 CAKK22 pKa = 10.69 LFDD25 pKa = 4.02 RR26 pKa = 11.84 TSKK29 pKa = 10.41 FSKK32 pKa = 10.12 AQRR35 pKa = 11.84 LLAASLKK42 pKa = 10.34 LIAEE46 pKa = 4.66 IMPDD50 pKa = 2.77 IPDD53 pKa = 3.22 RR54 pKa = 11.84 HH55 pKa = 5.84 FPAIRR60 pKa = 11.84 KK61 pKa = 5.72 YY62 pKa = 9.19 TKK64 pKa = 9.2 ATKK67 pKa = 10.36 NLATTAIKK75 pKa = 10.48 NHH77 pKa = 6.31 SLTQAKK83 pKa = 10.07 AKK85 pKa = 10.28 LIRR88 pKa = 11.84 EE89 pKa = 3.85 EE90 pKa = 4.35 RR91 pKa = 11.84 YY92 pKa = 9.9 NDD94 pKa = 3.57 RR95 pKa = 11.84 VCIPNARR102 pKa = 11.84 QVAATGDD109 pKa = 3.69 YY110 pKa = 10.32 QRR112 pKa = 11.84 TSVVVRR118 pKa = 11.84 STLRR122 pKa = 11.84 ATLNDD127 pKa = 3.55 VNDD130 pKa = 4.15 FTANIEE136 pKa = 4.14 YY137 pKa = 9.75 HH138 pKa = 6.98 RR139 pKa = 11.84 KK140 pKa = 8.55 RR141 pKa = 11.84 QTVPEE146 pKa = 4.13 LQRR149 pKa = 11.84 EE150 pKa = 4.31 LRR152 pKa = 11.84 RR153 pKa = 11.84 MNEE156 pKa = 3.86 TLAEE160 pKa = 4.17 LPALVQQGYY169 pKa = 8.96 NRR171 pKa = 11.84 YY172 pKa = 9.12 CDD174 pKa = 3.99 EE175 pKa = 4.71 LRR177 pKa = 11.84 HH178 pKa = 4.7 PTIWKK183 pKa = 8.11 QKK185 pKa = 8.93 KK186 pKa = 8.0 RR187 pKa = 11.84 QKK189 pKa = 9.23 EE190 pKa = 4.09 TLTKK194 pKa = 10.39 LEE196 pKa = 3.82 KK197 pKa = 10.33 SEE199 pKa = 4.43 RR200 pKa = 11.84 DD201 pKa = 3.2 RR202 pKa = 11.84 KK203 pKa = 10.82 AKK205 pKa = 10.35 FQVKK209 pKa = 9.6 GGAGMWQRR217 pKa = 11.84 LRR219 pKa = 11.84 QQKK222 pKa = 10.04
Molecular weight: 25.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.235
IPC2_protein 9.545
IPC_protein 9.706
Toseland 10.584
ProMoST 10.131
Dawson 10.672
Bjellqvist 10.277
Wikipedia 10.789
Rodwell 11.155
Grimsley 10.701
Solomon 10.716
Lehninger 10.701
Nozaki 10.555
DTASelect 10.277
Thurlkill 10.555
EMBOSS 10.95
Sillero 10.584
Patrickios 10.862
IPC_peptide 10.716
IPC2_peptide 8.902
IPC2.peptide.svr19 8.643
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
16
0
16
9936
222
1312
621.0
70.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.958 ± 0.173
1.329 ± 0.204
5.847 ± 0.211
5.304 ± 0.18
4.136 ± 0.258
4.64 ± 0.187
3.09 ± 0.183
7.377 ± 0.318
4.469 ± 0.471
9.541 ± 0.285
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.526 ± 0.162
5.435 ± 0.201
4.68 ± 0.281
4.147 ± 0.184
6.079 ± 0.329
6.924 ± 0.294
7.146 ± 0.362
6.159 ± 0.2
0.896 ± 0.069
4.318 ± 0.248
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here