Budvicia aquatica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4968 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2C6D1A9|A0A2C6D1A9_9GAMM Autotransporter outer membrane beta-barrel domain-containing protein OS=Budvicia aquatica OX=82979 GN=CRN84_15370 PE=4 SV=1
MM1 pKa = 7.54LLSMLITGSASAAVTSSPTLTVKK24 pKa = 10.43GRR26 pKa = 11.84APVVAQSTIVSDD38 pKa = 3.72NANGNGLLDD47 pKa = 4.43AGDD50 pKa = 3.99TLSVSTVGGFSDD62 pKa = 4.73ADD64 pKa = 3.32GDD66 pKa = 4.04AATPEE71 pKa = 4.42TYY73 pKa = 9.48QWQADD78 pKa = 3.81GVDD81 pKa = 3.2ISGATSQTYY90 pKa = 10.28GIVVGDD96 pKa = 3.98LGKK99 pKa = 8.55TLTLLVTPNTDD110 pKa = 3.31PAITDD115 pKa = 3.78PSVGSPVASNGLTVIAGGTVTDD137 pKa = 3.76VAITGDD143 pKa = 3.36VGGYY147 pKa = 8.23PQVDD151 pKa = 4.33SILTATATCAGGTCNGVTYY170 pKa = 10.11QWEE173 pKa = 4.35IEE175 pKa = 4.22TGLGSNTYY183 pKa = 8.47STISGATSNTYY194 pKa = 9.65TPVRR198 pKa = 11.84TDD200 pKa = 2.86QKK202 pKa = 11.04LKK204 pKa = 9.97VQVTVSNALRR214 pKa = 3.9

Molecular weight: 21.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2C6DJI2|A0A2C6DJI2_9GAMM Membrane protein FxsA OS=Budvicia aquatica OX=82979 GN=fxsA PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.55GRR39 pKa = 11.84TRR41 pKa = 11.84LSASSS46 pKa = 3.21

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4968

0

4968

1615998

29

6271

325.3

35.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.701 ± 0.042

1.078 ± 0.018

5.32 ± 0.03

5.249 ± 0.048

3.826 ± 0.033

7.706 ± 0.074

2.079 ± 0.02

6.711 ± 0.028

4.644 ± 0.033

10.375 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.617 ± 0.023

4.552 ± 0.061

4.099 ± 0.039

4.408 ± 0.035

4.789 ± 0.047

6.813 ± 0.043

5.758 ± 0.066

6.835 ± 0.036

1.329 ± 0.015

3.113 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski