Acinetobacter stercoris
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3857 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U3N1N8|A0A2U3N1N8_9GAMM Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase OS=Acinetobacter stercoris OX=2126983 GN=lgt PE=3 SV=1
MM1 pKa = 7.37 GKK3 pKa = 8.14 VTATDD8 pKa = 3.82 GAGNTGPEE16 pKa = 4.25 ATTGAADD23 pKa = 3.4 VTAPVAPTVDD33 pKa = 3.55 QNNAAGLGGTTEE45 pKa = 4.19 PNAVITVEE53 pKa = 4.36 LGNGSTVTTTADD65 pKa = 3.42 SQGNWLLEE73 pKa = 4.28 PNPLAEE79 pKa = 4.35 GEE81 pKa = 4.43 VGKK84 pKa = 8.63 VTATDD89 pKa = 3.8 GAGNSSTPTMTDD101 pKa = 2.73 GTDD104 pKa = 2.75 ITAPVAPTVDD114 pKa = 3.55 HH115 pKa = 6.82 NNAAGLGGTTEE126 pKa = 4.19 PNAVITVEE134 pKa = 4.36 LGNGSTVTTTADD146 pKa = 3.48 SQGNWNIKK154 pKa = 9.15 PNPIAEE160 pKa = 4.5 GEE162 pKa = 4.4 SATVIATDD170 pKa = 3.52 KK171 pKa = 11.23 AGNTSAEE178 pKa = 4.36 TPTGKK183 pKa = 10.45 ADD185 pKa = 3.77 TVAPTAPGIDD195 pKa = 3.55 QNNEE199 pKa = 3.73 SGLSGTAEE207 pKa = 4.14 PEE209 pKa = 4.03 STISVEE215 pKa = 4.14 LADD218 pKa = 4.02 GTTITTTTDD227 pKa = 2.92 TAGNWSITPNPLGDD241 pKa = 3.81 GEE243 pKa = 4.53 VGKK246 pKa = 8.62 VTATDD251 pKa = 3.38 AAGNNSAPTMTDD263 pKa = 2.7 GTDD266 pKa = 3.19 ITPPAAPGVDD276 pKa = 3.42 QNNGEE281 pKa = 4.36 GLSGTAEE288 pKa = 3.99 AGATVTVKK296 pKa = 10.73 DD297 pKa = 3.6 ADD299 pKa = 4.11 GNTVTTTADD308 pKa = 3.54 STGHH312 pKa = 5.99 WSITPNPISDD322 pKa = 3.67 GMSGTVTATDD332 pKa = 3.45 EE333 pKa = 4.79 AGNEE337 pKa = 4.35 SNPSNTGTADD347 pKa = 3.29 TVAPNAPLVEE357 pKa = 4.24 QNNAAGLAGTTEE369 pKa = 4.29 PNAVITVDD377 pKa = 4.15 LGNGSTVTTMADD389 pKa = 3.57 SQGHH393 pKa = 5.15 WSIEE397 pKa = 3.99 PNPLEE402 pKa = 4.11 TGEE405 pKa = 4.25 VGKK408 pKa = 8.62 VTATDD413 pKa = 3.42 AAGNTSTPTMTDD425 pKa = 2.87 GTDD428 pKa = 3.19 ITPPAAPGIDD438 pKa = 3.42 QNNGEE443 pKa = 4.58 GLSGTAEE450 pKa = 3.99 AGATVTVKK458 pKa = 10.73 DD459 pKa = 3.6 ADD461 pKa = 4.11 GNTVTTTADD470 pKa = 3.54 STGHH474 pKa = 5.99 WSITPNPIADD484 pKa = 3.95 GTSGTVTATDD494 pKa = 3.41 EE495 pKa = 4.64 AGNEE499 pKa = 4.12 SSPSNTGTADD509 pKa = 3.29 TVAPNAPLVEE519 pKa = 4.24 QNNAAGLAGTTEE531 pKa = 4.29 PNAVITVDD539 pKa = 4.15 LGNGSTVTTMADD551 pKa = 3.57 SQGHH555 pKa = 5.15 WSIEE559 pKa = 3.99 PNPLEE564 pKa = 4.11 TGEE567 pKa = 4.25 VGKK570 pKa = 8.62 VTATDD575 pKa = 3.42 AAGNTSTPTMTDD587 pKa = 2.87 GTDD590 pKa = 3.19 ITPPAAPGVDD600 pKa = 3.42 QNNGEE605 pKa = 4.36 GLSGTAEE612 pKa = 3.99 AGATVTVKK620 pKa = 10.73 DD621 pKa = 3.6 ADD623 pKa = 4.11 GNTVTTTADD632 pKa = 3.54 STGHH636 pKa = 5.99 WSITPNPITDD646 pKa = 3.61 GSEE649 pKa = 4.22 GSVTATDD656 pKa = 3.58 AAGNTSAEE664 pKa = 4.55 TPTGTADD671 pKa = 3.51 TVAPTAPGIDD681 pKa = 3.53 QNNGEE686 pKa = 4.58 GLSGTAEE693 pKa = 3.99 AGATVTVKK701 pKa = 10.73 DD702 pKa = 3.6 ADD704 pKa = 4.11 GNTVTTTADD713 pKa = 3.54 STGHH717 pKa = 5.99 WSITPNPITDD727 pKa = 3.86 GTSGTVTATDD737 pKa = 3.41 EE738 pKa = 4.64 AGNEE742 pKa = 4.12 SSPSNTGIADD752 pKa = 4.02 TVAPTAPGIDD762 pKa = 3.53 QNNGEE767 pKa = 4.41 GLSGTVEE774 pKa = 3.88 AGATVIVKK782 pKa = 10.21 DD783 pKa = 3.6 ADD785 pKa = 3.83 GNTVTTTADD794 pKa = 3.54 STGHH798 pKa = 5.99 WSITPNPLADD808 pKa = 3.81 GSEE811 pKa = 4.35 GSVTATDD818 pKa = 3.58 AAGNTSAEE826 pKa = 4.55 TPTGTADD833 pKa = 3.51 TVAPTAPGIDD843 pKa = 3.53 QNNGEE848 pKa = 4.58 GLSGTAEE855 pKa = 3.99 AGATVTVKK863 pKa = 10.73 DD864 pKa = 3.6 ADD866 pKa = 4.11 GNTVTTTADD875 pKa = 3.54 STGHH879 pKa = 5.99 WSITPNPITDD889 pKa = 3.86 GTSGTVTATDD899 pKa = 3.41 EE900 pKa = 4.64 AGNEE904 pKa = 4.05 SSPTNTGIADD914 pKa = 4.18 TVAPTAPGIDD924 pKa = 3.53 QNNGEE929 pKa = 4.63 GLTGTAEE936 pKa = 4.15 AGSTVIVKK944 pKa = 10.26 DD945 pKa = 3.34 ADD947 pKa = 3.83 GNTVTTTADD956 pKa = 3.54 STGHH960 pKa = 5.99 WSITPNPITDD970 pKa = 3.61 GSEE973 pKa = 4.22 GSVTATDD980 pKa = 3.58 AAGNTSAEE988 pKa = 4.55 TPTGTADD995 pKa = 4.48 LVAPDD1000 pKa = 4.27 KK1001 pKa = 11.15 PLVNLVNGIDD1011 pKa = 4.65 PITGTAEE1018 pKa = 3.88 PNSIVTVTYY1027 pKa = 11.07 LDD1029 pKa = 3.91 GTSVTVTASSDD1040 pKa = 3.76 GSWSVLNPGLEE1051 pKa = 4.58 DD1052 pKa = 4.31 GDD1054 pKa = 4.71 TITVTATDD1062 pKa = 3.59 EE1063 pKa = 4.24 VGNKK1067 pKa = 9.61 SEE1069 pKa = 4.43 SVTVTVDD1076 pKa = 3.12 TYY1078 pKa = 11.72 VLADD1082 pKa = 3.45 TVIITNGVTNDD1093 pKa = 3.57 DD1094 pKa = 3.61 TPAIVGTAEE1103 pKa = 3.99 ANSIVTLTIIGTTGSQVVSVSVDD1126 pKa = 3.18 GTGVWTYY1133 pKa = 10.24 TPTVALAEE1141 pKa = 4.09 DD1142 pKa = 5.12 VYY1144 pKa = 10.53 TLSAIARR1151 pKa = 11.84 DD1152 pKa = 3.56 SEE1154 pKa = 4.26 GRR1156 pKa = 11.84 VSQIGASSITVDD1168 pKa = 3.29 TTPPSSEE1175 pKa = 3.47 IVIEE1179 pKa = 4.02 ITAITDD1185 pKa = 3.39 DD1186 pKa = 4.21 SGVLGDD1192 pKa = 5.9 FITNDD1197 pKa = 3.61 TTPTIHH1203 pKa = 6.06 GTITGTLSADD1213 pKa = 3.02 EE1214 pKa = 4.63 HH1215 pKa = 6.66 AQISIDD1221 pKa = 5.35 NGVTWINLAIVDD1233 pKa = 4.54 GKK1235 pKa = 9.89 WSYY1238 pKa = 11.86 NEE1240 pKa = 3.69 TRR1242 pKa = 11.84 TSVDD1246 pKa = 4.82 GIDD1249 pKa = 3.17 QTYY1252 pKa = 7.95 TYY1254 pKa = 9.72 QVRR1257 pKa = 11.84 VVDD1260 pKa = 3.57 NAGNVGSTDD1269 pKa = 3.43 SQVVKK1274 pKa = 10.61 ISTTVPDD1281 pKa = 3.36 NTITVTTLLTADD1293 pKa = 3.69 TTPTISGAYY1302 pKa = 9.71 AKK1304 pKa = 10.74 ALGNGEE1310 pKa = 4.36 TIQVIVNGVTYY1321 pKa = 10.22 KK1322 pKa = 10.71 QGDD1325 pKa = 4.05 GYY1327 pKa = 10.32 LTVNTTAKK1335 pKa = 8.73 TWSLHH1340 pKa = 6.08 IPDD1343 pKa = 5.07 ANALNVNGATDD1354 pKa = 3.59 TPYY1357 pKa = 10.95 SIIAQIVNIAGNVVSDD1373 pKa = 3.6 TTANEE1378 pKa = 3.75 LTVYY1382 pKa = 10.57 QDD1384 pKa = 3.27 VSISAGPSPDD1394 pKa = 3.72 YY1395 pKa = 10.44 MGQQPLFNGMTKK1407 pKa = 9.77 IGAGEE1412 pKa = 3.85 KK1413 pKa = 10.74 LEE1415 pKa = 4.21 VYY1417 pKa = 10.06 VYY1419 pKa = 11.1 NSTGTLIKK1427 pKa = 9.94 TFSSVNGASVVDD1439 pKa = 3.8 GLVFDD1444 pKa = 4.62 ATTGSWKK1451 pKa = 8.42 ITAANWGTSQLAGGSYY1467 pKa = 8.64 TVQAKK1472 pKa = 9.26 VVVADD1477 pKa = 4.28 GSGGQISDD1485 pKa = 3.48 VQAITVIQPIAITVPNSTYY1504 pKa = 11.03 SDD1506 pKa = 3.51 INSKK1510 pKa = 10.47 VYY1512 pKa = 10.77 ALDD1515 pKa = 4.15 DD1516 pKa = 4.08 GGYY1519 pKa = 10.67 LLIWAQNTNNNTGSAASNYY1538 pKa = 10.36 DD1539 pKa = 3.6 LVAQRR1544 pKa = 11.84 YY1545 pKa = 4.52 TQSGVAVGSLIKK1557 pKa = 9.58 ITNTTTGTYY1566 pKa = 10.64 ANSEE1570 pKa = 4.36 GYY1572 pKa = 10.79 ADD1574 pKa = 5.53 RR1575 pKa = 11.84 NDD1577 pKa = 3.3 MWDD1580 pKa = 3.44 SNGSYY1585 pKa = 10.01 SANVNGDD1592 pKa = 3.13 GSFTIAYY1599 pKa = 9.42 NYY1601 pKa = 11.04 DD1602 pKa = 3.6 LATQSAIKK1610 pKa = 10.82 SFDD1613 pKa = 3.37 DD1614 pKa = 4.03 SGNLLSTTTVNSGQNYY1630 pKa = 7.84 YY1631 pKa = 10.67 FGYY1634 pKa = 10.7 NYY1636 pKa = 10.25 VEE1638 pKa = 4.19 TTNGKK1643 pKa = 9.4 VVVFTSGTIHH1653 pKa = 7.79 DD1654 pKa = 4.4 YY1655 pKa = 11.3 DD1656 pKa = 3.67 IFIAGSVKK1664 pKa = 10.45 SYY1666 pKa = 10.67 SASNLTNEE1674 pKa = 4.45 TNGGNGYY1681 pKa = 10.03 IYY1683 pKa = 10.38 NQYY1686 pKa = 8.79 ATPTGYY1692 pKa = 10.59 SGFGNSTNIEE1702 pKa = 4.24 SVSAVSLGGSLVLVQYY1718 pKa = 11.58 ADD1720 pKa = 3.68 YY1721 pKa = 9.66 TSTDD1725 pKa = 3.12 AYY1727 pKa = 7.74 PTKK1730 pKa = 9.93 EE1731 pKa = 3.56 NVMIVDD1737 pKa = 3.79 YY1738 pKa = 10.97 SSTGVATVLKK1748 pKa = 10.34 SAVANYY1754 pKa = 9.88 YY1755 pKa = 11.02 DD1756 pKa = 4.04 NGWQIGACSFVLKK1769 pKa = 10.7 EE1770 pKa = 4.17 GGFVSLWAGNQDD1782 pKa = 3.32 SATSLTNGGTMDD1794 pKa = 4.1 GFNVYY1799 pKa = 8.35 SRR1801 pKa = 11.84 RR1802 pKa = 11.84 FSYY1805 pKa = 10.82 DD1806 pKa = 2.56 VGANTITALDD1816 pKa = 3.8 TTEE1819 pKa = 4.91 KK1820 pKa = 10.23 MVNTTMNGVNGVGYY1834 pKa = 8.1 STMSTGHH1841 pKa = 6.22 FSGCALAQGGYY1852 pKa = 8.6 VVVWTKK1858 pKa = 10.18 MLSNSLSEE1866 pKa = 4.88 VYY1868 pKa = 10.33 SQSYY1872 pKa = 9.55 DD1873 pKa = 3.16 AAGNRR1878 pKa = 11.84 LGGEE1882 pKa = 4.48 TLVSTQTVDD1891 pKa = 3.0 GTGTIDD1897 pKa = 3.46 TLPTVTALKK1906 pKa = 10.72 DD1907 pKa = 3.19 GGYY1910 pKa = 9.52 VVSWTNSASTSYY1922 pKa = 10.87 AINSTTTDD1930 pKa = 2.74 IKK1932 pKa = 10.07 TVIVNGDD1939 pKa = 3.34 GTLRR1943 pKa = 11.84 GADD1946 pKa = 3.47 EE1947 pKa = 4.24 THH1949 pKa = 7.0 SIDD1952 pKa = 3.31 ATYY1955 pKa = 11.36 LNGTGTLIGTDD1966 pKa = 4.59 GVCTLDD1972 pKa = 3.48 GRR1974 pKa = 11.84 NGATTMIGGKK1984 pKa = 10.22 DD1985 pKa = 3.31 NDD1987 pKa = 3.92 NIIIKK1992 pKa = 9.89 DD1993 pKa = 3.47 INFSHH1998 pKa = 7.4 IDD2000 pKa = 3.26 GGAGNDD2006 pKa = 3.59 TLIWDD2011 pKa = 4.14 SSANLNLGDD2020 pKa = 5.39 ILDD2023 pKa = 3.88 KK2024 pKa = 10.81 VQNIEE2029 pKa = 4.26 TIHH2032 pKa = 7.7 LGDD2035 pKa = 4.12 ANANTMTVSIDD2046 pKa = 5.18 DD2047 pKa = 3.92 ILQMTSNNTLIIQGGDD2063 pKa = 3.08 TDD2065 pKa = 3.75 KK2066 pKa = 11.6 VQIVDD2071 pKa = 3.66 ANSWTSGGTQSYY2083 pKa = 10.32 HH2084 pKa = 5.59 GADD2087 pKa = 3.59 YY2088 pKa = 10.89 QIYY2091 pKa = 8.01 TATTSDD2097 pKa = 2.99 NHH2099 pKa = 7.29 LINLWIQNNLGVIAA2113 pKa = 5.11
Molecular weight: 213.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.699
IPC2_protein 3.605
IPC_protein 3.668
Toseland 3.427
ProMoST 3.821
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.605
Rodwell 3.478
Grimsley 3.325
Solomon 3.656
Lehninger 3.617
Nozaki 3.757
DTASelect 4.05
Thurlkill 3.478
EMBOSS 3.605
Sillero 3.783
Patrickios 1.481
IPC_peptide 3.656
IPC2_peptide 3.757
IPC2.peptide.svr19 3.713
Protein with the highest isoelectric point:
>tr|A0A2U3N3H8|A0A2U3N3H8_9GAMM Elongation factor G OS=Acinetobacter stercoris OX=2126983 GN=fusA PE=3 SV=1
MM1 pKa = 7.31 IQTLTAIQTPTATQTRR17 pKa = 11.84 TAXQTRR23 pKa = 11.84 TAIQTXTATQTXTXIQTPTATQTPTVIQTXTAIQTXTATQTRR65 pKa = 11.84 TVIQTLTAIQTPTXTQTLTAXQXRR89 pKa = 11.84 TAIQTLTATQTXTXXQTXTXIQTLTAIQTPTATQTPTVIQTLTAIQTPTXTQTRR143 pKa = 11.84 TXIQTPTXTQTRR155 pKa = 11.84 TATQTPTVIQTLTATQTLTATQTLTVTQTPTLMNRR190 pKa = 11.84 MILHH194 pKa = 6.52 LMKK197 pKa = 9.99 MVQNLQVQQKK207 pKa = 10.14 LALQLLL213 pKa = 4.27
Molecular weight: 21.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.468
IPC2_protein 11.023
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.398
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.149
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.12
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3857
0
3857
1176768
30
4370
305.1
34.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.103 ± 0.049
0.923 ± 0.013
5.332 ± 0.042
5.783 ± 0.038
4.384 ± 0.034
6.529 ± 0.047
2.373 ± 0.023
7.314 ± 0.031
5.969 ± 0.038
10.172 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.405 ± 0.023
4.805 ± 0.041
3.891 ± 0.028
5.06 ± 0.041
4.097 ± 0.033
6.367 ± 0.036
5.385 ± 0.045
6.473 ± 0.033
1.261 ± 0.017
3.368 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here