Elysia chlorotica (Eastern emerald elysia) (Sea slug)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Spiralia; Lophotrochozoa; Mollusca; Gastropoda; Heterobranchia; Euthyneura; Panpulmonata; Sacoglossa; Placobranchoidea; Plakobranchidae; Elysia

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23836 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S1I3S8|A0A3S1I3S8_ELYCH Uncharacterized protein OS=Elysia chlorotica OX=188477 GN=EGW08_000503 PE=4 SV=1
MM1 pKa = 7.4KK2 pKa = 9.73PAVNPVSTINKK13 pKa = 6.69TLPGVTGHH21 pKa = 5.8VHH23 pKa = 5.79HH24 pKa = 7.01HH25 pKa = 6.09WSCGGTIVSISHH37 pKa = 7.07PLGCLSSFTVEE48 pKa = 4.33MKK50 pKa = 10.31TGMSDD55 pKa = 4.36DD56 pKa = 4.24GACGGDD62 pKa = 3.1GGGGFKK68 pKa = 11.0DD69 pKa = 3.55EE70 pKa = 5.6DD71 pKa = 3.92EE72 pKa = 4.43YY73 pKa = 11.55DD74 pKa = 3.67YY75 pKa = 11.68DD76 pKa = 3.61SGGGCRR82 pKa = 11.84VGGVGDD88 pKa = 4.29CGVSGGGGCGFGGGGGFGLGGGGGFGVGGVGVCGVGGVGGVDD130 pKa = 3.69YY131 pKa = 11.43GNDD134 pKa = 3.73AADD137 pKa = 4.56DD138 pKa = 5.11GYY140 pKa = 11.53DD141 pKa = 3.28SAAADD146 pKa = 4.6GDD148 pKa = 3.96DD149 pKa = 4.44DD150 pKa = 4.79QNGDD154 pKa = 3.33GDD156 pKa = 4.36YY157 pKa = 10.8EE158 pKa = 4.07AAAAAAAAAADD169 pKa = 5.07DD170 pKa = 5.87DD171 pKa = 6.36DD172 pKa = 7.71DD173 pKa = 7.66DD174 pKa = 7.66DD175 pKa = 7.66DD176 pKa = 7.65DD177 pKa = 7.65DD178 pKa = 7.65DD179 pKa = 7.65DD180 pKa = 7.65DD181 pKa = 7.65DD182 pKa = 7.65DD183 pKa = 7.65DD184 pKa = 7.65DD185 pKa = 7.65DD186 pKa = 7.61DD187 pKa = 7.6DD188 pKa = 7.65DD189 pKa = 7.6DD190 pKa = 5.71DD191 pKa = 5.5DD192 pKa = 6.05CGGVDD197 pKa = 4.32DD198 pKa = 4.49MMIVVSRR205 pKa = 11.84RR206 pKa = 11.84MVQIAPQLPGRR217 pKa = 11.84LKK219 pKa = 10.86KK220 pKa = 10.65EE221 pKa = 4.27GGSGPTTKK229 pKa = 10.29AYY231 pKa = 8.29PQTRR235 pKa = 11.84VLYY238 pKa = 10.96AEE240 pKa = 5.31VNDD243 pKa = 3.81QCGEE247 pKa = 3.77RR248 pKa = 11.84STNRR252 pKa = 11.84FCGSLRR258 pKa = 11.84TFRR261 pKa = 11.84LIEE264 pKa = 4.26PKK266 pKa = 9.51WNPISQPRR274 pKa = 11.84YY275 pKa = 7.23TVLWWSPNGVQPQTPCLSPPTSMSHH300 pKa = 5.3YY301 pKa = 10.11AYY303 pKa = 9.63IDD305 pKa = 3.27ATSRR309 pKa = 11.84EE310 pKa = 4.11QGDD313 pKa = 4.01PLWIGTQGFLTSFSGLDD330 pKa = 3.33MLVLSVSEE338 pKa = 3.96WLGLGVGGG346 pKa = 4.95

Molecular weight: 35.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A433U0U8|A0A433U0U8_ELYCH Uncharacterized protein OS=Elysia chlorotica OX=188477 GN=EGW08_004785 PE=4 SV=1
MM1 pKa = 7.08TPLVARR7 pKa = 11.84NIPPLVARR15 pKa = 11.84NILPLVARR23 pKa = 11.84NILPLVARR31 pKa = 11.84NIPPLVARR39 pKa = 11.84NILPLVAGNIQLLVARR55 pKa = 11.84HH56 pKa = 5.98ILPLVARR63 pKa = 11.84NILPLVARR71 pKa = 11.84NILPLVARR79 pKa = 11.84NILPLVARR87 pKa = 11.84NILPLVARR95 pKa = 11.84NIIRR99 pKa = 11.84LFARR103 pKa = 11.84HH104 pKa = 5.35NN105 pKa = 3.55

Molecular weight: 11.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23836

0

23836

10343547

50

9454

433.9

48.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.625 ± 0.014

2.11 ± 0.017

5.912 ± 0.02

6.198 ± 0.024

3.659 ± 0.013

6.296 ± 0.018

2.685 ± 0.01

4.567 ± 0.013

5.817 ± 0.02

8.777 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.364 ± 0.009

4.324 ± 0.013

5.288 ± 0.019

4.474 ± 0.016

5.617 ± 0.017

9.165 ± 0.029

5.897 ± 0.016

6.335 ± 0.018

1.087 ± 0.007

2.772 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski