Alternaria alternata chrysovirus 1
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5X7B8|A0A2Z5X7B8_9VIRU Uncharacterized protein OS=Alternaria alternata chrysovirus 1 OX=2066695 PE=4 SV=1
MM1 pKa = 7.96 ADD3 pKa = 3.24 QSEE6 pKa = 4.8 DD7 pKa = 3.75 LPPSYY12 pKa = 11.15 SLTPNTTSSSPSARR26 pKa = 11.84 PFEE29 pKa = 4.61 RR30 pKa = 11.84 MMEE33 pKa = 4.16 ALTVRR38 pKa = 11.84 SAAVTTWSEE47 pKa = 3.95 QVEE50 pKa = 4.37 AEE52 pKa = 4.24 AEE54 pKa = 3.92 EE55 pKa = 4.9 GLQQEE60 pKa = 4.72 VSVGGTSLKK69 pKa = 10.37 LARR72 pKa = 11.84 LDD74 pKa = 3.35 YY75 pKa = 11.36 GMARR79 pKa = 11.84 DD80 pKa = 3.77 PVPPQEE86 pKa = 3.83 RR87 pKa = 11.84 LQYY90 pKa = 10.53 QRR92 pKa = 11.84 GLRR95 pKa = 11.84 GTTLLRR101 pKa = 11.84 TAASRR106 pKa = 11.84 AAVPCSDD113 pKa = 4.08 CEE115 pKa = 3.91 ISRR118 pKa = 11.84 VCAGVPYY125 pKa = 10.39 GPGVKK130 pKa = 10.13 SLGWTKK136 pKa = 11.04 AEE138 pKa = 4.06 QKK140 pKa = 10.9 ASLVVLDD147 pKa = 5.23 TVASMLVRR155 pKa = 11.84 GFRR158 pKa = 11.84 KK159 pKa = 9.71 GVSKK163 pKa = 8.32 MTLPKK168 pKa = 10.26 GSRR171 pKa = 11.84 VTLSEE176 pKa = 3.84 WPEE179 pKa = 3.52 QNLYY183 pKa = 8.24 HH184 pKa = 6.31 TEE186 pKa = 3.69 VGYY189 pKa = 7.82 TWWSAVLVAPSDD201 pKa = 3.75 NIIRR205 pKa = 11.84 VMMHH209 pKa = 5.95 VDD211 pKa = 3.38 GTTLLDD217 pKa = 3.24 IAGGDD222 pKa = 3.47 EE223 pKa = 4.09 MLEE226 pKa = 4.08 ARR228 pKa = 11.84 HH229 pKa = 6.42 RR230 pKa = 11.84 DD231 pKa = 3.57 PDD233 pKa = 3.7 LHH235 pKa = 7.88 SDD237 pKa = 4.37 LLVLLAGVQHH247 pKa = 7.34 AILADD252 pKa = 3.76 EE253 pKa = 5.02 LSASYY258 pKa = 10.71 HH259 pKa = 5.97 LLQDD263 pKa = 3.53 ASLATMRR270 pKa = 11.84 QHH272 pKa = 7.28 VSTTSAGSTDD282 pKa = 3.18 QGGYY286 pKa = 10.67 AGGGPNHH293 pKa = 6.68 ALWSEE298 pKa = 4.06 GEE300 pKa = 4.23 YY301 pKa = 10.98 LSDD304 pKa = 5.59 DD305 pKa = 3.65 LVQDD309 pKa = 3.41 RR310 pKa = 11.84 RR311 pKa = 11.84 VPTAYY316 pKa = 10.78 YY317 pKa = 10.24 DD318 pKa = 3.76 ANLDD322 pKa = 3.47 LTAYY326 pKa = 9.7 GLASAGDD333 pKa = 4.09 LPPAVLVALGANGGKK348 pKa = 10.28 SITGSVLTLRR358 pKa = 11.84 NSSAAGGFFLAVTSATANAKK378 pKa = 9.93 PYY380 pKa = 10.84 ALEE383 pKa = 4.37 KK384 pKa = 10.52 SLKK387 pKa = 10.45 LGATVRR393 pKa = 11.84 DD394 pKa = 4.0 GLFSPACVGALCSRR408 pKa = 11.84 APGLVGVVVDD418 pKa = 3.8 NEE420 pKa = 4.45 YY421 pKa = 10.73 IAMQTVLVRR430 pKa = 11.84 LDD432 pKa = 3.78 PEE434 pKa = 4.9 DD435 pKa = 4.24 PLVLYY440 pKa = 10.76 DD441 pKa = 3.84 RR442 pKa = 11.84 DD443 pKa = 3.64 PYY445 pKa = 11.14 RR446 pKa = 11.84 LYY448 pKa = 10.74 EE449 pKa = 4.19 YY450 pKa = 10.98 VWDD453 pKa = 3.96 LCGVMGWRR461 pKa = 11.84 IEE463 pKa = 4.03 AAAGYY468 pKa = 10.73 AMEE471 pKa = 5.06 VYY473 pKa = 10.71 SGDD476 pKa = 3.38 TRR478 pKa = 11.84 LGDD481 pKa = 3.31 VAVRR485 pKa = 11.84 YY486 pKa = 8.63 GAAVGPRR493 pKa = 11.84 WLRR496 pKa = 11.84 LLTLAAIEE504 pKa = 4.09 PRR506 pKa = 11.84 KK507 pKa = 10.24 SRR509 pKa = 11.84 WSPEE513 pKa = 3.61 LLLDD517 pKa = 3.83 YY518 pKa = 10.41 GASGLGRR525 pKa = 11.84 YY526 pKa = 8.07 LLRR529 pKa = 11.84 EE530 pKa = 4.15 YY531 pKa = 10.64 ILAGRR536 pKa = 11.84 FAGFKK541 pKa = 10.14 SLMMHH546 pKa = 5.65 TEE548 pKa = 4.54 GYY550 pKa = 9.29 FAEE553 pKa = 4.41 AAKK556 pKa = 10.59 SIASPKK562 pKa = 9.44 PNAPAAKK569 pKa = 9.95 LPVSYY574 pKa = 11.06 VIDD577 pKa = 3.61 TEE579 pKa = 4.23 YY580 pKa = 10.28 LTRR583 pKa = 11.84 KK584 pKa = 9.9 LPDD587 pKa = 3.32 GSIEE591 pKa = 4.16 RR592 pKa = 11.84 FVYY595 pKa = 10.75 ALGLAKK601 pKa = 9.95 FYY603 pKa = 10.97 AGNYY607 pKa = 8.2 VGSALVVDD615 pKa = 4.59 QSPEE619 pKa = 3.96 LEE621 pKa = 4.77 QFIQDD626 pKa = 3.86 NEE628 pKa = 4.28 HH629 pKa = 6.54 LAGTKK634 pKa = 9.33 PMRR637 pKa = 11.84 MLASIRR643 pKa = 11.84 EE644 pKa = 4.25 EE645 pKa = 3.72 IAPRR649 pKa = 11.84 ASLCNGNPSALLAEE663 pKa = 4.41 LRR665 pKa = 11.84 IAARR669 pKa = 11.84 SPEE672 pKa = 3.65 VRR674 pKa = 11.84 LYY676 pKa = 11.36 AKK678 pKa = 10.46 GADD681 pKa = 3.69 AEE683 pKa = 4.41 RR684 pKa = 11.84 EE685 pKa = 4.14 LLSSKK690 pKa = 10.11 LARR693 pKa = 11.84 GTRR696 pKa = 11.84 LFRR699 pKa = 11.84 RR700 pKa = 11.84 ANLAAPLRR708 pKa = 11.84 VPLHH712 pKa = 6.18 EE713 pKa = 5.35 LGAMVPRR720 pKa = 11.84 YY721 pKa = 9.85 EE722 pKa = 4.04 EE723 pKa = 3.94 LAAKK727 pKa = 9.95 HH728 pKa = 6.49 DD729 pKa = 4.39 WDD731 pKa = 4.86 PSHH734 pKa = 6.81 NPAKK738 pKa = 10.21 EE739 pKa = 3.88 CVLFGIEE746 pKa = 4.71 AGLGDD751 pKa = 4.53 ALPTEE756 pKa = 4.5 RR757 pKa = 11.84 AVGLDD762 pKa = 3.65 DD763 pKa = 5.14 MYY765 pKa = 11.58 TLLPQMSTQVVV776 pKa = 3.27
Molecular weight: 83.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.288
IPC2_protein 5.347
IPC_protein 5.347
Toseland 5.436
ProMoST 5.537
Dawson 5.41
Bjellqvist 5.512
Wikipedia 5.321
Rodwell 5.347
Grimsley 5.436
Solomon 5.41
Lehninger 5.385
Nozaki 5.575
DTASelect 5.741
Thurlkill 5.512
EMBOSS 5.461
Sillero 5.664
Patrickios 3.999
IPC_peptide 5.423
IPC2_peptide 5.664
IPC2.peptide.svr19 5.618
Protein with the highest isoelectric point:
>tr|A0A2Z5X7J9|A0A2Z5X7J9_9VIRU Uncharacterized protein OS=Alternaria alternata chrysovirus 1 OX=2066695 PE=4 SV=1
MM1 pKa = 7.05 MFWNKK6 pKa = 9.24 RR7 pKa = 11.84 QAAPGVMGSEE17 pKa = 4.07 RR18 pKa = 11.84 MKK20 pKa = 10.42 VAGLIRR26 pKa = 11.84 PATAVSTVPWWFLDD40 pKa = 3.63 TPLRR44 pKa = 11.84 TEE46 pKa = 4.32 ASALCLLLTIWTHH59 pKa = 6.68 LRR61 pKa = 11.84 GACLAICDD69 pKa = 4.17 RR70 pKa = 11.84 RR71 pKa = 11.84 RR72 pKa = 11.84 SSDD75 pKa = 3.1 QCSEE79 pKa = 3.73 CRR81 pKa = 11.84 ACVARR86 pKa = 11.84 DD87 pKa = 3.19 WRR89 pKa = 11.84 HH90 pKa = 4.15 TAWRR94 pKa = 11.84 HH95 pKa = 4.95 LRR97 pKa = 11.84 WSLVAAGRR105 pKa = 11.84 PDD107 pKa = 3.4 ALRR110 pKa = 11.84 GVNFGG115 pKa = 3.81
Molecular weight: 13.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.309
IPC2_protein 9.209
IPC_protein 10.101
Toseland 10.701
ProMoST 10.716
Dawson 10.73
Bjellqvist 10.584
Wikipedia 11.023
Rodwell 10.628
Grimsley 10.745
Solomon 11.038
Lehninger 10.979
Nozaki 10.789
DTASelect 10.54
Thurlkill 10.701
EMBOSS 11.14
Sillero 10.73
Patrickios 10.496
IPC_peptide 11.038
IPC2_peptide 10.262
IPC2.peptide.svr19 8.923
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3553
115
1117
710.6
77.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.468 ± 0.262
1.407 ± 0.219
4.7 ± 0.339
6.445 ± 0.552
2.533 ± 0.422
7.543 ± 0.613
2.336 ± 0.313
2.252 ± 0.054
3.096 ± 0.457
10.667 ± 0.698
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.589 ± 0.108
2.083 ± 0.262
5.545 ± 0.817
3.8 ± 0.405
7.149 ± 0.303
7.656 ± 0.433
4.869 ± 0.314
8.19 ± 0.317
1.52 ± 0.261
3.152 ± 0.419
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here