Haloquadratum walsbyi (strain DSM 16790 / HBSQ001)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Haloferacaceae; Haloquadratum; Haloquadratum walsbyi

Average proteome isoelectric point is 4.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2558 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q18E23|Q18E23_HALWD Probable membrane transporter protein OS=Haloquadratum walsbyi (strain DSM 16790 / HBSQ001) OX=362976 GN=HQ_3724A PE=3 SV=1
MM1 pKa = 6.86TTDD4 pKa = 5.21DD5 pKa = 3.75YY6 pKa = 12.07AKK8 pKa = 10.53DD9 pKa = 3.65VLVSPEE15 pKa = 4.02WVTDD19 pKa = 3.64RR20 pKa = 11.84LDD22 pKa = 4.48EE23 pKa = 4.37IQSDD27 pKa = 3.91DD28 pKa = 3.52PDD30 pKa = 3.67YY31 pKa = 11.39RR32 pKa = 11.84LVEE35 pKa = 3.99VDD37 pKa = 4.33VDD39 pKa = 3.75TEE41 pKa = 4.55AYY43 pKa = 10.35DD44 pKa = 3.53EE45 pKa = 4.18AHH47 pKa = 6.81APGAIGFNWEE57 pKa = 4.25TQLQDD62 pKa = 3.16PTTRR66 pKa = 11.84DD67 pKa = 3.23ILDD70 pKa = 3.83KK71 pKa = 11.32EE72 pKa = 4.33DD73 pKa = 4.8FEE75 pKa = 4.87TLLGNHH81 pKa = 7.56GISDD85 pKa = 4.28DD86 pKa = 3.9STVIIYY92 pKa = 10.65GDD94 pKa = 3.44NSNWFASYY102 pKa = 9.16TYY104 pKa = 9.6WQFKK108 pKa = 10.46YY109 pKa = 10.45YY110 pKa = 10.89GHH112 pKa = 7.87DD113 pKa = 3.89DD114 pKa = 3.4VRR116 pKa = 11.84LLNGARR122 pKa = 11.84DD123 pKa = 3.57YY124 pKa = 11.05WLDD127 pKa = 3.03NDD129 pKa = 4.16YY130 pKa = 10.94PVTDD134 pKa = 4.23EE135 pKa = 4.06VPEE138 pKa = 4.14YY139 pKa = 10.59STVEE143 pKa = 3.89YY144 pKa = 9.8EE145 pKa = 3.8ARR147 pKa = 11.84GPFEE151 pKa = 4.77SIRR154 pKa = 11.84AYY156 pKa = 10.72RR157 pKa = 11.84DD158 pKa = 3.77DD159 pKa = 3.78VDD161 pKa = 4.12HH162 pKa = 7.51AIDD165 pKa = 3.57QDD167 pKa = 4.05LPLVDD172 pKa = 3.5VRR174 pKa = 11.84SPEE177 pKa = 4.06EE178 pKa = 3.62FSGEE182 pKa = 3.6ILAPPGLNEE191 pKa = 3.53TAQRR195 pKa = 11.84GGHH198 pKa = 6.15IPGASNISWADD209 pKa = 3.47TVNDD213 pKa = 4.04DD214 pKa = 4.14GTFKK218 pKa = 10.79SAEE221 pKa = 4.14EE222 pKa = 4.35LEE224 pKa = 4.83SIYY227 pKa = 11.12ADD229 pKa = 3.58YY230 pKa = 11.09DD231 pKa = 3.33IDD233 pKa = 4.29GSEE236 pKa = 4.11TTVAYY241 pKa = 10.32CRR243 pKa = 11.84IGEE246 pKa = 4.16RR247 pKa = 11.84SSIAWFALHH256 pKa = 6.85EE257 pKa = 4.13LLGYY261 pKa = 10.7DD262 pKa = 3.91EE263 pKa = 4.55VVNYY267 pKa = 10.35DD268 pKa = 3.71GSWTEE273 pKa = 3.62WGNLVGAPIEE283 pKa = 4.34TGGADD288 pKa = 3.04

Molecular weight: 32.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q18KL4|Q18KL4_HALWD SnoaL-like domain-containing protein OS=Haloquadratum walsbyi (strain DSM 16790 / HBSQ001) OX=362976 GN=HQ_1303A PE=4 SV=1
MM1 pKa = 7.53ARR3 pKa = 11.84NEE5 pKa = 3.96AAIKK9 pKa = 9.23RR10 pKa = 11.84TLEE13 pKa = 3.63KK14 pKa = 10.14QICMRR19 pKa = 11.84CNARR23 pKa = 11.84NPQRR27 pKa = 11.84ASEE30 pKa = 4.11CRR32 pKa = 11.84KK33 pKa = 9.96CGYY36 pKa = 10.66SNLRR40 pKa = 11.84PKK42 pKa = 10.65SKK44 pKa = 10.08EE45 pKa = 3.37RR46 pKa = 11.84RR47 pKa = 11.84AAA49 pKa = 3.42

Molecular weight: 5.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2558

0

2558

772216

19

9159

301.9

32.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.398 ± 0.053

0.755 ± 0.018

7.819 ± 0.057

7.451 ± 0.08

3.201 ± 0.038

7.495 ± 0.077

2.092 ± 0.029

6.198 ± 0.046

2.335 ± 0.033

8.184 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.811 ± 0.024

3.443 ± 0.048

4.358 ± 0.035

3.429 ± 0.031

5.77 ± 0.049

6.82 ± 0.066

7.898 ± 0.08

7.801 ± 0.048

1.006 ± 0.019

2.736 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski