Desulfovibrio alkalitolerans DSM 16529

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfovibrionales; Desulfovibrionaceae; Desulfohalovibrio; Desulfohalovibrio alkalitolerans

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2924 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S7US07|S7US07_9DELT Beta-phosphoglucomutase family hydrolase OS=Desulfovibrio alkalitolerans DSM 16529 OX=1121439 GN=dsat_2461 PE=4 SV=1
MM1 pKa = 7.47SIDD4 pKa = 3.43VSLQALHH11 pKa = 6.73AFSLAQAVTANNVANVEE28 pKa = 4.4TPGFQASQTVLEE40 pKa = 4.94SGPEE44 pKa = 3.82DD45 pKa = 3.45RR46 pKa = 11.84GVRR49 pKa = 11.84VAAIVPDD56 pKa = 4.2EE57 pKa = 4.52TPGPPVPGYY66 pKa = 9.01PSPDD70 pKa = 3.52ALDD73 pKa = 3.17MSTYY77 pKa = 10.7DD78 pKa = 3.09NSYY81 pKa = 11.66AEE83 pKa = 4.16GSNVNLAGEE92 pKa = 4.52MVDD95 pKa = 5.36LMTAQRR101 pKa = 11.84AYY103 pKa = 9.91EE104 pKa = 4.15ANLVSIASYY113 pKa = 11.1DD114 pKa = 3.64QMTGTVLDD122 pKa = 3.84IMAA125 pKa = 5.52

Molecular weight: 13.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S7UGR1|S7UGR1_9DELT DD-transpeptidase OS=Desulfovibrio alkalitolerans DSM 16529 OX=1121439 GN=dsat_1017 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.07QPSKK9 pKa = 9.83IRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.38RR14 pKa = 11.84TLGFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.3AGRR28 pKa = 11.84QVIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.91GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2924

0

2924

955238

30

1819

326.7

35.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.591 ± 0.063

1.298 ± 0.022

5.431 ± 0.032

6.617 ± 0.042

3.982 ± 0.031

8.332 ± 0.034

2.097 ± 0.018

4.644 ± 0.034

3.749 ± 0.041

10.966 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.615 ± 0.021

2.46 ± 0.028

5.119 ± 0.033

2.868 ± 0.023

7.412 ± 0.05

5.186 ± 0.03

4.777 ± 0.026

7.454 ± 0.037

1.164 ± 0.019

2.239 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski