Afipia sp. P52-10

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Bradyrhizobiaceae; Afipia; unclassified Afipia

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4769 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W3RI01|W3RI01_9BRAD Hva1_TUDOR domain-containing protein OS=Afipia sp. P52-10 OX=1429916 GN=X566_03260 PE=4 SV=1
MM1 pKa = 7.38LSVVADD7 pKa = 4.6CAPVWDD13 pKa = 4.88PRR15 pKa = 11.84DD16 pKa = 3.66PAQSRR21 pKa = 11.84ANPSTWQISYY31 pKa = 11.11NPILHH36 pKa = 7.18LIDD39 pKa = 5.31YY40 pKa = 8.62ITEE43 pKa = 4.29PDD45 pKa = 3.32GGLGLEE51 pKa = 4.36YY52 pKa = 9.5EE53 pKa = 4.65TVIEE57 pKa = 4.12PVLNAWMAEE66 pKa = 3.47ADD68 pKa = 3.71LCDD71 pKa = 3.82EE72 pKa = 4.49RR73 pKa = 11.84VATASGGTEE82 pKa = 3.46PRR84 pKa = 11.84YY85 pKa = 8.52TSNGWYY91 pKa = 10.11QFDD94 pKa = 4.37NEE96 pKa = 4.43PKK98 pKa = 10.57DD99 pKa = 3.81VINAILATCDD109 pKa = 2.72GWLAEE114 pKa = 4.79AGDD117 pKa = 3.75GTLAVKK123 pKa = 10.42VGVYY127 pKa = 9.93RR128 pKa = 11.84EE129 pKa = 4.14PTVTLTQNEE138 pKa = 4.17IFDD141 pKa = 4.25FSLSYY146 pKa = 10.37GQPDD150 pKa = 3.54EE151 pKa = 4.24QAVNN155 pKa = 3.46

Molecular weight: 17.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W3RMF8|W3RMF8_9BRAD Glycosyl transferase OS=Afipia sp. P52-10 OX=1429916 GN=X566_12070 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84LATTGGRR28 pKa = 11.84KK29 pKa = 9.06VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4769

0

4769

1435595

27

2668

301.0

32.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.683 ± 0.05

0.83 ± 0.011

5.484 ± 0.029

5.205 ± 0.039

3.747 ± 0.025

8.324 ± 0.042

1.978 ± 0.02

5.411 ± 0.024

3.653 ± 0.031

9.847 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.504 ± 0.018

2.718 ± 0.02

5.279 ± 0.029

3.281 ± 0.023

7.084 ± 0.035

5.414 ± 0.023

5.492 ± 0.026

7.484 ± 0.03

1.304 ± 0.015

2.273 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski