Amino acid dipepetide frequency for Afipia sp. P52-10

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.721AlaAla: 17.721 ± 0.193	1.094AlaCys: 1.094 ± 0.03	6.932AlaAsp: 6.932 ± 0.07	6.904AlaGlu: 6.904 ± 0.097	4.632AlaPhe: 4.632 ± 0.072	10.325AlaGly: 10.325 ± 0.106	2.243AlaHis: 2.243 ± 0.045	7.01AlaIle: 7.01 ± 0.08	4.921AlaLys: 4.921 ± 0.076	13.146AlaLeu: 13.146 ± 0.139	3.677AlaMet: 3.677 ± 0.053	3.204AlaAsn: 3.204 ± 0.048	5.695AlaPro: 5.695 ± 0.084	4.402AlaGln: 4.402 ± 0.054	8.607AlaArg: 8.607 ± 0.086	6.856AlaSer: 6.856 ± 0.072	6.558AlaThr: 6.558 ± 0.068	8.751AlaVal: 8.751 ± 0.085	1.527AlaTrp: 1.527 ± 0.032	2.626AlaTyr: 2.626 ± 0.042	0.004AlaXaa: 0.004 ± 0.002
Cys	0.995CysAla: 0.995 ± 0.027	0.132CysCys: 0.132 ± 0.009	0.513CysAsp: 0.513 ± 0.02	0.433CysGlu: 0.433 ± 0.016	0.291CysPhe: 0.291 ± 0.014	0.862CysGly: 0.862 ± 0.026	0.234CysHis: 0.234 ± 0.012	0.447CysIle: 0.447 ± 0.018	0.224CysLys: 0.224 ± 0.014	0.725CysLeu: 0.725 ± 0.022	0.18CysMet: 0.18 ± 0.011	0.241CysAsn: 0.241 ± 0.013	0.406CysPro: 0.406 ± 0.017	0.216CysGln: 0.216 ± 0.011	0.605CysArg: 0.605 ± 0.021	0.467CysSer: 0.467 ± 0.019	0.396CysThr: 0.396 ± 0.018	0.631CysVal: 0.631 ± 0.024	0.107CysTrp: 0.107 ± 0.008	0.196CysTyr: 0.196 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	6.79AspAla: 6.79 ± 0.075	0.452AspCys: 0.452 ± 0.018	3.117AspAsp: 3.117 ± 0.058	3.112AspGlu: 3.112 ± 0.054	2.054AspPhe: 2.054 ± 0.045	4.863AspGly: 4.863 ± 0.052	1.215AspHis: 1.215 ± 0.033	3.127AspIle: 3.127 ± 0.049	1.961AspLys: 1.961 ± 0.038	5.33AspLeu: 5.33 ± 0.061	1.248AspMet: 1.248 ± 0.029	1.298AspAsn: 1.298 ± 0.032	3.357AspPro: 3.357 ± 0.048	1.643AspGln: 1.643 ± 0.037	4.078AspArg: 4.078 ± 0.059	2.109AspSer: 2.109 ± 0.038	2.564AspThr: 2.564 ± 0.049	4.225AspVal: 4.225 ± 0.056	0.841AspTrp: 0.841 ± 0.023	1.455AspTyr: 1.455 ± 0.031	0.0AspXaa: 0.0 ± 0.0
Glu	6.779GluAla: 6.779 ± 0.086	0.319GluCys: 0.319 ± 0.015	2.28GluAsp: 2.28 ± 0.039	2.566GluGlu: 2.566 ± 0.052	1.74GluPhe: 1.74 ± 0.035	3.651GluGly: 3.651 ± 0.053	1.111GluHis: 1.111 ± 0.031	3.27GluIle: 3.27 ± 0.05	2.166GluLys: 2.166 ± 0.047	4.868GluLeu: 4.868 ± 0.065	1.438GluMet: 1.438 ± 0.029	1.408GluAsn: 1.408 ± 0.035	2.556GluPro: 2.556 ± 0.048	2.258GluGln: 2.258 ± 0.048	4.999GluArg: 4.999 ± 0.071	2.21GluSer: 2.21 ± 0.037	3.274GluThr: 3.274 ± 0.048	3.558GluVal: 3.558 ± 0.059	0.64GluTrp: 0.64 ± 0.02	0.961GluTyr: 0.961 ± 0.03	0.002GluXaa: 0.002 ± 0.001
Phe	5.014PheAla: 5.014 ± 0.061	0.423PheCys: 0.423 ± 0.017	2.556PheAsp: 2.556 ± 0.047	1.992PheGlu: 1.992 ± 0.044	1.365PhePhe: 1.365 ± 0.031	3.804PheGly: 3.804 ± 0.066	0.701PheHis: 0.701 ± 0.023	1.73PheIle: 1.73 ± 0.042	1.236PheLys: 1.236 ± 0.032	3.278PheLeu: 3.278 ± 0.05	0.816PheMet: 0.816 ± 0.023	1.133PheAsn: 1.133 ± 0.033	1.624PhePro: 1.624 ± 0.028	0.995PheGln: 0.995 ± 0.029	2.254PheArg: 2.254 ± 0.039	2.145PheSer: 2.145 ± 0.043	2.03PheThr: 2.03 ± 0.043	3.02PheVal: 3.02 ± 0.045	0.527PheTrp: 0.527 ± 0.02	0.832PheTyr: 0.832 ± 0.025	0.001PheXaa: 0.001 ± 0.001
Gly	9.159GlyAla: 9.159 ± 0.089	0.802GlyCys: 0.802 ± 0.022	4.057GlyAsp: 4.057 ± 0.048	4.277GlyGlu: 4.277 ± 0.057	3.672GlyPhe: 3.672 ± 0.054	7.387GlyGly: 7.387 ± 0.115	1.753GlyHis: 1.753 ± 0.035	4.698GlyIle: 4.698 ± 0.06	3.448GlyLys: 3.448 ± 0.055	8.492GlyLeu: 8.492 ± 0.099	2.182GlyMet: 2.182 ± 0.045	2.169GlyAsn: 2.169 ± 0.045	3.426GlyPro: 3.426 ± 0.045	2.819GlyGln: 2.819 ± 0.047	5.775GlyArg: 5.775 ± 0.066	4.542GlySer: 4.542 ± 0.059	4.677GlyThr: 4.677 ± 0.067	6.074GlyVal: 6.074 ± 0.073	1.364GlyTrp: 1.364 ± 0.034	2.455GlyTyr: 2.455 ± 0.045	0.006GlyXaa: 0.006 ± 0.002
His	2.397HisAla: 2.397 ± 0.042	0.219HisCys: 0.219 ± 0.013	1.193HisAsp: 1.193 ± 0.032	0.958HisGlu: 0.958 ± 0.027	0.757HisPhe: 0.757 ± 0.024	1.865HisGly: 1.865 ± 0.035	0.6HisHis: 0.6 ± 0.023	0.935HisIle: 0.935 ± 0.028	0.469HisLys: 0.469 ± 0.02	1.984HisLeu: 1.984 ± 0.04	0.497HisMet: 0.497 ± 0.018	0.464HisAsn: 0.464 ± 0.017	1.312HisPro: 1.312 ± 0.029	0.586HisGln: 0.586 ± 0.018	1.469HisArg: 1.469 ± 0.037	0.823HisSer: 0.823 ± 0.025	0.899HisThr: 0.899 ± 0.028	1.504HisVal: 1.504 ± 0.034	0.306HisTrp: 0.306 ± 0.017	0.541HisTyr: 0.541 ± 0.019	0.001HisXaa: 0.001 ± 0.001
Ile	8.146IleAla: 8.146 ± 0.083	0.487IleCys: 0.487 ± 0.02	3.723IleAsp: 3.723 ± 0.049	3.556IleGlu: 3.556 ± 0.054	1.698IlePhe: 1.698 ± 0.04	5.303IleGly: 5.303 ± 0.065	0.879IleHis: 0.879 ± 0.026	2.46IleIle: 2.46 ± 0.051	1.84IleLys: 1.84 ± 0.037	4.283IleLeu: 4.283 ± 0.057	0.965IleMet: 0.965 ± 0.024	1.567IleAsn: 1.567 ± 0.035	2.469IlePro: 2.469 ± 0.038	1.245IleGln: 1.245 ± 0.028	3.233IleArg: 3.233 ± 0.047	2.871IleSer: 2.871 ± 0.046	2.755IleThr: 2.755 ± 0.045	4.878IleVal: 4.878 ± 0.063	0.61IleTrp: 0.61 ± 0.022	1.145IleTyr: 1.145 ± 0.03	0.001IleXaa: 0.001 ± 0.001
Lys	4.574LysAla: 4.574 ± 0.071	0.158LysCys: 0.158 ± 0.01	2.05LysAsp: 2.05 ± 0.045	1.768LysGlu: 1.768 ± 0.036	1.021LysPhe: 1.021 ± 0.028	2.692LysGly: 2.692 ± 0.049	0.729LysHis: 0.729 ± 0.022	1.857LysIle: 1.857 ± 0.04	1.485LysLys: 1.485 ± 0.049	3.897LysLeu: 3.897 ± 0.056	0.815LysMet: 0.815 ± 0.024	0.995LysAsn: 0.995 ± 0.029	2.543LysPro: 2.543 ± 0.045	1.291LysGln: 1.291 ± 0.035	2.786LysArg: 2.786 ± 0.052	1.975LysSer: 1.975 ± 0.037	2.228LysThr: 2.228 ± 0.047	2.636LysVal: 2.636 ± 0.045	0.352LysTrp: 0.352 ± 0.018	0.676LysTyr: 0.676 ± 0.021	0.001LysXaa: 0.001 ± 0.001
Leu	13.436LeuAla: 13.436 ± 0.139	0.876LeuCys: 0.876 ± 0.024	5.485LeuAsp: 5.485 ± 0.07	4.421LeuGlu: 4.421 ± 0.07	3.581LeuPhe: 3.581 ± 0.064	8.027LeuGly: 8.027 ± 0.086	1.784LeuHis: 1.784 ± 0.036	5.136LeuIle: 5.136 ± 0.066	3.832LeuLys: 3.832 ± 0.056	9.377LeuLeu: 9.377 ± 0.126	2.398LeuMet: 2.398 ± 0.045	2.662LeuAsn: 2.662 ± 0.048	5.398LeuPro: 5.398 ± 0.068	3.019LeuGln: 3.019 ± 0.043	6.67LeuArg: 6.67 ± 0.08	6.258LeuSer: 6.258 ± 0.073	5.738LeuThr: 5.738 ± 0.068	7.277LeuVal: 7.277 ± 0.077	1.108LeuTrp: 1.108 ± 0.033	1.983LeuTyr: 1.983 ± 0.039	0.001LeuXaa: 0.001 ± 0.001
Met	3.013MetAla: 3.013 ± 0.044	0.171MetCys: 0.171 ± 0.01	1.034MetAsp: 1.034 ± 0.025	0.997MetGlu: 0.997 ± 0.03	0.818MetPhe: 0.818 ± 0.021	1.609MetGly: 1.609 ± 0.032	0.443MetHis: 0.443 ± 0.019	1.495MetIle: 1.495 ± 0.034	1.0MetLys: 1.0 ± 0.027	2.655MetLeu: 2.655 ± 0.042	0.699MetMet: 0.699 ± 0.024	0.756MetAsn: 0.756 ± 0.023	1.568MetPro: 1.568 ± 0.03	0.961MetGln: 0.961 ± 0.028	1.899MetArg: 1.899 ± 0.035	1.676MetSer: 1.676 ± 0.034	1.973MetThr: 1.973 ± 0.037	1.705MetVal: 1.705 ± 0.033	0.247MetTrp: 0.247 ± 0.011	0.324MetTyr: 0.324 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.474AsnAla: 3.474 ± 0.056	0.219AsnCys: 0.219 ± 0.012	1.494AsnAsp: 1.494 ± 0.036	1.345AsnGlu: 1.345 ± 0.033	0.957AsnPhe: 0.957 ± 0.024	2.489AsnGly: 2.489 ± 0.05	0.525AsnHis: 0.525 ± 0.019	1.452AsnIle: 1.452 ± 0.032	0.918AsnLys: 0.918 ± 0.028	2.565AsnLeu: 2.565 ± 0.046	0.626AsnMet: 0.626 ± 0.019	0.76AsnAsn: 0.76 ± 0.024	1.868AsnPro: 1.868 ± 0.038	0.755AsnGln: 0.755 ± 0.024	1.841AsnArg: 1.841 ± 0.039	1.197AsnSer: 1.197 ± 0.03	1.35AsnThr: 1.35 ± 0.033	2.162AsnVal: 2.162 ± 0.038	0.422AsnTrp: 0.422 ± 0.018	0.757AsnTyr: 0.757 ± 0.024	0.002AsnXaa: 0.002 ± 0.001
Pro	6.487ProAla: 6.487 ± 0.086	0.29ProCys: 0.29 ± 0.015	3.425ProAsp: 3.425 ± 0.053	3.121ProGlu: 3.121 ± 0.047	2.07ProPhe: 2.07 ± 0.037	4.262ProGly: 4.262 ± 0.062	1.096ProHis: 1.096 ± 0.026	2.476ProIle: 2.476 ± 0.045	2.008ProLys: 2.008 ± 0.044	4.832ProLeu: 4.832 ± 0.058	1.212ProMet: 1.212 ± 0.028	1.573ProAsn: 1.573 ± 0.033	3.014ProPro: 3.014 ± 0.083	1.927ProGln: 1.927 ± 0.035	3.206ProArg: 3.206 ± 0.052	2.936ProSer: 2.936 ± 0.049	2.669ProThr: 2.669 ± 0.047	4.232ProVal: 4.232 ± 0.054	0.685ProTrp: 0.685 ± 0.022	1.268ProTyr: 1.268 ± 0.033	0.001ProXaa: 0.001 ± 0.001
Gln	4.377GlnAla: 4.377 ± 0.054	0.245GlnCys: 0.245 ± 0.011	1.438GlnAsp: 1.438 ± 0.035	1.379GlnGlu: 1.379 ± 0.029	1.139GlnPhe: 1.139 ± 0.027	2.368GlnGly: 2.368 ± 0.037	0.727GlnHis: 0.727 ± 0.024	1.87GlnIle: 1.87 ± 0.037	1.124GlnLys: 1.124 ± 0.031	3.011GlnLeu: 3.011 ± 0.048	0.923GlnMet: 0.923 ± 0.024	0.898GlnAsn: 0.898 ± 0.028	1.922GlnPro: 1.922 ± 0.042	1.406GlnGln: 1.406 ± 0.038	2.838GlnArg: 2.838 ± 0.047	1.829GlnSer: 1.829 ± 0.034	1.905GlnThr: 1.905 ± 0.036	2.329GlnVal: 2.329 ± 0.046	0.424GlnTrp: 0.424 ± 0.017	0.654GlnTyr: 0.654 ± 0.024	0.001GlnXaa: 0.001 ± 0.001
Arg	8.197ArgAla: 8.197 ± 0.077	0.541ArgCys: 0.541 ± 0.019	4.0ArgAsp: 4.0 ± 0.063	4.085ArgGlu: 4.085 ± 0.059	2.903ArgPhe: 2.903 ± 0.045	4.845ArgGly: 4.845 ± 0.056	1.544ArgHis: 1.544 ± 0.035	4.088ArgIle: 4.088 ± 0.053	2.637ArgLys: 2.637 ± 0.046	7.359ArgLeu: 7.359 ± 0.082	1.93ArgMet: 1.93 ± 0.04	2.002ArgAsn: 2.002 ± 0.044	3.63ArgPro: 3.63 ± 0.052	2.533ArgGln: 2.533 ± 0.047	5.561ArgArg: 5.561 ± 0.077	3.822ArgSer: 3.822 ± 0.055	3.583ArgThr: 3.583 ± 0.049	4.735ArgVal: 4.735 ± 0.06	1.046ArgTrp: 1.046 ± 0.028	1.799ArgTyr: 1.799 ± 0.036	0.001ArgXaa: 0.001 ± 0.001
Ser	6.455SerAla: 6.455 ± 0.061	0.418SerCys: 0.418 ± 0.019	2.922SerAsp: 2.922 ± 0.04	2.767SerGlu: 2.767 ± 0.055	2.3SerPhe: 2.3 ± 0.041	5.371SerGly: 5.371 ± 0.077	1.034SerHis: 1.034 ± 0.028	2.937SerIle: 2.937 ± 0.049	1.725SerLys: 1.725 ± 0.039	5.375SerLeu: 5.375 ± 0.072	1.369SerMet: 1.369 ± 0.033	1.424SerAsn: 1.424 ± 0.032	2.864SerPro: 2.864 ± 0.047	1.653SerGln: 1.653 ± 0.033	3.579SerArg: 3.579 ± 0.058	3.103SerSer: 3.103 ± 0.055	2.697SerThr: 2.697 ± 0.038	4.037SerVal: 4.037 ± 0.061	0.752SerTrp: 0.752 ± 0.024	1.36SerTyr: 1.36 ± 0.031	0.001SerXaa: 0.001 ± 0.001
Thr	6.611ThrAla: 6.611 ± 0.075	0.443ThrCys: 0.443 ± 0.018	2.706ThrAsp: 2.706 ± 0.046	2.566ThrGlu: 2.566 ± 0.044	2.219ThrPhe: 2.219 ± 0.042	4.92ThrGly: 4.92 ± 0.068	1.002ThrHis: 1.002 ± 0.03	3.231ThrIle: 3.231 ± 0.053	1.737ThrLys: 1.737 ± 0.04	5.966ThrLeu: 5.966 ± 0.07	1.333ThrMet: 1.333 ± 0.031	1.403ThrAsn: 1.403 ± 0.029	3.333ThrPro: 3.333 ± 0.053	1.571ThrGln: 1.571 ± 0.034	3.422ThrArg: 3.422 ± 0.048	3.041ThrSer: 3.041 ± 0.048	3.048ThrThr: 3.048 ± 0.047	4.43ThrVal: 4.43 ± 0.053	0.661ThrTrp: 0.661 ± 0.021	1.28ThrTyr: 1.28 ± 0.034	0.001ThrXaa: 0.001 ± 0.001
Val	9.466ValAla: 9.466 ± 0.099	0.646ValCys: 0.646 ± 0.021	3.805ValAsp: 3.805 ± 0.061	4.145ValGlu: 4.145 ± 0.058	2.806ValPhe: 2.806 ± 0.049	5.535ValGly: 5.535 ± 0.086	1.394ValHis: 1.394 ± 0.032	4.21ValIle: 4.21 ± 0.062	2.597ValLys: 2.597 ± 0.046	7.447ValLeu: 7.447 ± 0.082	1.93ValMet: 1.93 ± 0.034	2.178ValAsn: 2.178 ± 0.042	3.859ValPro: 3.859 ± 0.053	2.223ValGln: 2.223 ± 0.036	5.095ValArg: 5.095 ± 0.058	4.335ValSer: 4.335 ± 0.05	4.6ValThr: 4.6 ± 0.056	6.199ValVal: 6.199 ± 0.078	0.838ValTrp: 0.838 ± 0.025	1.53ValTyr: 1.53 ± 0.036	0.006ValXaa: 0.006 ± 0.002
Trp	1.098TrpAla: 1.098 ± 0.03	0.134TrpCys: 0.134 ± 0.01	0.597TrpAsp: 0.597 ± 0.02	0.488TrpGlu: 0.488 ± 0.02	0.515TrpPhe: 0.515 ± 0.018	0.87TrpGly: 0.87 ± 0.028	0.321TrpHis: 0.321 ± 0.015	0.727TrpIle: 0.727 ± 0.023	0.471TrpLys: 0.471 ± 0.019	1.616TrpLeu: 1.616 ± 0.035	0.351TrpMet: 0.351 ± 0.015	0.429TrpAsn: 0.429 ± 0.018	0.763TrpPro: 0.763 ± 0.023	0.557TrpGln: 0.557 ± 0.023	1.149TrpArg: 1.149 ± 0.026	0.822TrpSer: 0.822 ± 0.026	0.86TrpThr: 0.86 ± 0.025	0.788TrpVal: 0.788 ± 0.022	0.22TrpTrp: 0.22 ± 0.014	0.265TrpTyr: 0.265 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.643TyrAla: 2.643 ± 0.046	0.232TyrCys: 0.232 ± 0.014	1.511TyrAsp: 1.511 ± 0.034	1.172TyrGlu: 1.172 ± 0.03	0.936TyrPhe: 0.936 ± 0.029	2.184TyrGly: 2.184 ± 0.038	0.444TyrHis: 0.444 ± 0.018	0.959TyrIle: 0.959 ± 0.025	0.73TyrLys: 0.73 ± 0.023	2.258TyrLeu: 2.258 ± 0.036	0.455TyrMet: 0.455 ± 0.018	0.615TyrAsn: 0.615 ± 0.021	1.18TyrPro: 1.18 ± 0.029	0.745TyrGln: 0.745 ± 0.026	1.773TyrArg: 1.773 ± 0.034	1.123TyrSer: 1.123 ± 0.029	1.117TyrThr: 1.117 ± 0.034	1.669TyrVal: 1.669 ± 0.035	0.364TyrTrp: 0.364 ± 0.016	0.619TyrTyr: 0.619 ± 0.026	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.002XaaAla: 0.002 ± 0.001	0.001XaaCys: 0.001 ± 0.001	0.001XaaAsp: 0.001 ± 0.001	0.001XaaGlu: 0.001 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.003XaaLeu: 0.003 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.003XaaPro: 0.003 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.003XaaArg: 0.003 ± 0.001	0.003XaaSer: 0.003 ± 0.001	0.004XaaThr: 0.004 ± 0.002	0.003XaaVal: 0.003 ± 0.001	0.001XaaTrp: 0.001 ± 0.001	0.001XaaTyr: 0.001 ± 0.001	0.001XaaXaa: 0.001 ± 0.002

Statistics based on 4769 proteins (1435595 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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