Aliidiomarina taiwanensis
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2309 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A432X9L8|A0A432X9L8_9GAMM 50S ribosomal protein L35 OS=Aliidiomarina taiwanensis OX=946228 GN=rpmI PE=3 SV=1
MM1 pKa = 7.44 TKK3 pKa = 10.29 FNQTAFSLVLAGVLVSGAAFSQTRR27 pKa = 11.84 VASSQPNWRR36 pKa = 11.84 ADD38 pKa = 3.38 ALFEE42 pKa = 4.3 TVTLSEE48 pKa = 4.93 GFTPDD53 pKa = 2.68 PWSYY57 pKa = 11.47 DD58 pKa = 3.56 LQAGGQNQATRR69 pKa = 11.84 LGDD72 pKa = 3.42 EE73 pKa = 4.12 CSGYY77 pKa = 10.5 INNSAPDD84 pKa = 3.22 VTLIYY89 pKa = 10.39 EE90 pKa = 4.56 GGSILDD96 pKa = 3.31 LHH98 pKa = 7.13 LYY100 pKa = 7.34 VTSNEE105 pKa = 3.98 DD106 pKa = 3.04 TTLVVSGPDD115 pKa = 4.14 GRR117 pKa = 11.84 WHH119 pKa = 6.97 CNDD122 pKa = 4.28 DD123 pKa = 4.34 AMGLNPMIIFEE134 pKa = 4.34 QPDD137 pKa = 3.23 GGQYY141 pKa = 10.27 DD142 pKa = 3.31 IWVGVYY148 pKa = 10.24 GSGDD152 pKa = 3.25 IVPATLHH159 pKa = 6.03 ISEE162 pKa = 5.47 LNPMDD167 pKa = 3.54
Molecular weight: 18.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.752
IPC2_protein 3.846
IPC_protein 3.821
Toseland 3.605
ProMoST 3.948
Dawson 3.821
Bjellqvist 4.024
Wikipedia 3.783
Rodwell 3.656
Grimsley 3.516
Solomon 3.808
Lehninger 3.77
Nozaki 3.948
DTASelect 4.19
Thurlkill 3.668
EMBOSS 3.783
Sillero 3.948
Patrickios 0.655
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.881
Protein with the highest isoelectric point:
>tr|A0A432X8H3|A0A432X8H3_9GAMM ATP synthase subunit b OS=Aliidiomarina taiwanensis OX=946228 GN=atpF PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVIKK11 pKa = 10.49 RR12 pKa = 11.84 KK13 pKa = 9.2 RR14 pKa = 11.84 DD15 pKa = 3.06 HH16 pKa = 6.58 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 NGRR28 pKa = 11.84 KK29 pKa = 8.96 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.94 GRR39 pKa = 11.84 KK40 pKa = 8.67 VLSAA44 pKa = 4.05
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.406
IPC2_protein 10.877
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.369
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.055
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2309
0
2309
771387
37
2406
334.1
37.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.787 ± 0.053
0.858 ± 0.015
5.046 ± 0.04
6.578 ± 0.053
3.96 ± 0.033
7.011 ± 0.046
2.481 ± 0.027
5.412 ± 0.036
4.096 ± 0.046
10.629 ± 0.07
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.529 ± 0.022
3.635 ± 0.033
4.367 ± 0.034
5.065 ± 0.046
5.544 ± 0.039
6.037 ± 0.035
5.401 ± 0.037
7.3 ± 0.042
1.329 ± 0.022
2.936 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here