Calorimonas adulescens

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Thermoanaerobacterales; Thermoanaerobacteraceae; Calorimonas

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2527 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5D8QCV7|A0A5D8QCV7_9THEO Phospho-N-acetylmuramoyl-pentapeptide-transferase OS=Calorimonas adulescens OX=2606906 GN=mraY PE=3 SV=1
MM1 pKa = 7.63HH2 pKa = 7.21FPDD5 pKa = 4.84DD6 pKa = 4.33LYY8 pKa = 11.71YY9 pKa = 10.81SDD11 pKa = 3.68DD12 pKa = 4.14HH13 pKa = 6.61EE14 pKa = 4.34WLKK17 pKa = 11.3VEE19 pKa = 4.51GSRR22 pKa = 11.84GIIGITEE29 pKa = 3.89YY30 pKa = 11.18AQAEE34 pKa = 4.48LGDD37 pKa = 3.92VVYY40 pKa = 11.23VEE42 pKa = 4.8LPSVGDD48 pKa = 3.52EE49 pKa = 4.93FEE51 pKa = 6.03AGDD54 pKa = 3.49NFGVVEE60 pKa = 4.37SVKK63 pKa = 10.68SVSDD67 pKa = 3.38LYY69 pKa = 10.73IPVSGRR75 pKa = 11.84VVEE78 pKa = 4.5VNEE81 pKa = 4.39EE82 pKa = 4.12LDD84 pKa = 4.35DD85 pKa = 4.58SPEE88 pKa = 4.18LVNQDD93 pKa = 3.74PYY95 pKa = 11.47GKK97 pKa = 9.76GWMIVVEE104 pKa = 4.37LDD106 pKa = 4.29DD107 pKa = 4.65PEE109 pKa = 4.18EE110 pKa = 4.17TADD113 pKa = 3.7LMSSAEE119 pKa = 3.92YY120 pKa = 10.5EE121 pKa = 4.39SFIKK125 pKa = 10.53EE126 pKa = 4.05SEE128 pKa = 3.97

Molecular weight: 14.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5D8QBD9|A0A5D8QBD9_9THEO FAD-dependent oxidoreductase OS=Calorimonas adulescens OX=2606906 GN=FWJ32_08925 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 8.56MTYY5 pKa = 8.56QPKK8 pKa = 8.61KK9 pKa = 8.23RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.76RR14 pKa = 11.84VHH16 pKa = 6.01GFRR19 pKa = 11.84NRR21 pKa = 11.84MRR23 pKa = 11.84TATGRR28 pKa = 11.84KK29 pKa = 7.77VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.35GRR39 pKa = 11.84KK40 pKa = 8.9RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2527

0

2527

750405

30

2092

297.0

33.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.805 ± 0.054

1.015 ± 0.021

5.981 ± 0.04

7.133 ± 0.052

3.894 ± 0.036

7.414 ± 0.046

1.426 ± 0.017

9.227 ± 0.053

7.08 ± 0.054

9.022 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.999 ± 0.021

4.844 ± 0.036

3.578 ± 0.033

2.364 ± 0.025

4.662 ± 0.031

5.789 ± 0.039

4.873 ± 0.035

7.163 ± 0.043

0.736 ± 0.019

3.996 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski