Staphylococcus virus 96
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q4ZBT9|Q4ZBT9_9CAUD ORF060 OS=Staphylococcus virus 96 OX=320842 PE=4 SV=1
MM1 pKa = 7.45 ATQKK5 pKa = 10.54 QVDD8 pKa = 4.13 YY9 pKa = 11.64 VMSLQEE15 pKa = 3.89 QLGLEE20 pKa = 4.28 DD21 pKa = 3.85 CEE23 pKa = 5.23 KK24 pKa = 10.6 YY25 pKa = 9.42 TDD27 pKa = 3.83 EE28 pKa = 4.01 QVKK31 pKa = 10.4 AMSHH35 pKa = 6.03 KK36 pKa = 10.06 EE37 pKa = 3.72 VSNVIEE43 pKa = 4.26 NYY45 pKa = 7.91 KK46 pKa = 9.56 TSIWDD51 pKa = 3.31 EE52 pKa = 3.94 EE53 pKa = 4.79 LYY55 pKa = 10.65 NEE57 pKa = 4.54 CMSFGLPNCC66 pKa = 4.51
Molecular weight: 7.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.046
IPC2_protein 4.342
IPC_protein 4.177
Toseland 4.037
ProMoST 4.291
Dawson 4.126
Bjellqvist 4.279
Wikipedia 3.986
Rodwell 4.024
Grimsley 3.948
Solomon 4.113
Lehninger 4.062
Nozaki 4.24
DTASelect 4.342
Thurlkill 4.05
EMBOSS 4.012
Sillero 4.291
Patrickios 0.846
IPC_peptide 4.113
IPC2_peptide 4.279
IPC2.peptide.svr19 4.185
Protein with the highest isoelectric point:
>tr|Q4ZBS7|Q4ZBS7_9CAUD ORF024 OS=Staphylococcus virus 96 OX=320842 PE=4 SV=1
MM1 pKa = 7.63 NILLLLEE8 pKa = 4.68 IIRR11 pKa = 11.84 RR12 pKa = 11.84 LQLLRR17 pKa = 11.84 RR18 pKa = 11.84 RR19 pKa = 11.84 VKK21 pKa = 10.55 KK22 pKa = 9.47 EE23 pKa = 3.69 RR24 pKa = 11.84 KK25 pKa = 8.83 RR26 pKa = 11.84 STRR29 pKa = 11.84 NKK31 pKa = 10.21 LRR33 pKa = 3.66
Molecular weight: 4.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.269
IPC2_protein 10.57
IPC_protein 12.135
Toseland 12.31
ProMoST 12.808
Dawson 12.31
Bjellqvist 12.31
Wikipedia 12.793
Rodwell 12.018
Grimsley 12.354
Solomon 12.808
Lehninger 12.705
Nozaki 12.31
DTASelect 12.31
Thurlkill 12.31
EMBOSS 12.808
Sillero 12.31
Patrickios 11.769
IPC_peptide 12.808
IPC2_peptide 11.798
IPC2.peptide.svr19 9.194
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
79
0
79
14130
33
1047
178.9
20.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.633 ± 0.341
0.538 ± 0.091
6.277 ± 0.316
7.403 ± 0.443
4.381 ± 0.255
5.598 ± 0.334
1.72 ± 0.165
7.488 ± 0.239
9.179 ± 0.337
7.615 ± 0.28
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.682 ± 0.21
6.525 ± 0.292
2.923 ± 0.224
4.14 ± 0.199
4.055 ± 0.224
6.03 ± 0.275
6.065 ± 0.266
6.249 ± 0.308
1.139 ± 0.152
4.36 ± 0.355
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here