Bordetella avium (strain 197N)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Alcaligenaceae; Bordetella; Bordetella avium

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3372 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2KUY1|Q2KUY1_BORA1 Uncharacterized protein (Fragment) OS=Bordetella avium (strain 197N) OX=360910 GN=BAV1057 PE=4 SV=1
MM1 pKa = 7.12RR2 pKa = 11.84TWMCLICGWVYY13 pKa = 11.09DD14 pKa = 4.61EE15 pKa = 4.94EE16 pKa = 6.63AGLPDD21 pKa = 3.89EE22 pKa = 5.81GIAPGTRR29 pKa = 11.84WEE31 pKa = 4.42DD32 pKa = 3.53VPPNWVCPEE41 pKa = 3.8CGARR45 pKa = 11.84KK46 pKa = 9.54EE47 pKa = 4.36DD48 pKa = 3.84FEE50 pKa = 5.52LMEE53 pKa = 4.22II54 pKa = 4.3

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2KTK6|Q2KTK6_BORA1 ABC transporter substrate-binding protein OS=Bordetella avium (strain 197N) OX=360910 GN=BAV3395 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.65TRR25 pKa = 11.84GGRR28 pKa = 11.84AILNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.68GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3372

0

3372

1096534

24

6460

325.2

35.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.517 ± 0.048

0.89 ± 0.014

5.299 ± 0.026

5.15 ± 0.042

3.407 ± 0.028

8.314 ± 0.049

2.128 ± 0.023

4.697 ± 0.033

3.002 ± 0.033

11.105 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.441 ± 0.022

2.761 ± 0.032

5.165 ± 0.037

4.207 ± 0.029

7.119 ± 0.05

5.581 ± 0.037

4.936 ± 0.032

7.415 ± 0.04

1.423 ± 0.019

2.443 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski