Acinetobacter defluvii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Moraxellaceae; Acinetobacter

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3434 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S2FGM9|A0A2S2FGM9_9GAMM Acyl-CoA thioesterase OS=Acinetobacter defluvii OX=1871111 GN=DJ533_15900 PE=3 SV=1
MM1 pKa = 7.37SIVWNEE7 pKa = 3.45IPEE10 pKa = 4.91FIQQYY15 pKa = 10.07KK16 pKa = 10.48DD17 pKa = 3.23QILATKK23 pKa = 10.35KK24 pKa = 10.57SAIEE28 pKa = 4.27LNLTPCDD35 pKa = 6.08DD36 pKa = 3.64LTLWQSKK43 pKa = 10.23IGGQPYY49 pKa = 10.42LPVGVDD55 pKa = 3.56YY56 pKa = 10.27PCNEE60 pKa = 4.16NGEE63 pKa = 4.26ALQLIAQINFAEE75 pKa = 4.56LPNNQIYY82 pKa = 9.64PSQGILQFYY91 pKa = 10.47IDD93 pKa = 4.19TQDD96 pKa = 4.26DD97 pKa = 4.35LLGLDD102 pKa = 5.48FDD104 pKa = 5.41DD105 pKa = 4.04QQKK108 pKa = 10.6QNGFRR113 pKa = 11.84VIYY116 pKa = 9.24FADD119 pKa = 4.28VIADD123 pKa = 3.75QARR126 pKa = 11.84LQHH129 pKa = 6.61DD130 pKa = 4.6FPTEE134 pKa = 3.81EE135 pKa = 4.42LHH137 pKa = 7.65EE138 pKa = 4.52EE139 pKa = 3.95MSPIAGQYY147 pKa = 9.74AVQFAATEE155 pKa = 4.4RR156 pKa = 11.84YY157 pKa = 9.4ISIGDD162 pKa = 3.78HH163 pKa = 5.9EE164 pKa = 5.04FSSKK168 pKa = 10.37IFDD171 pKa = 4.32PYY173 pKa = 11.22AIDD176 pKa = 3.93EE177 pKa = 4.8DD178 pKa = 4.27LLEE181 pKa = 4.5EE182 pKa = 5.3DD183 pKa = 4.71EE184 pKa = 5.94LFDD187 pKa = 4.81GYY189 pKa = 11.45EE190 pKa = 4.75DD191 pKa = 3.76NFSSNGHH198 pKa = 6.24HH199 pKa = 6.85LGGYY203 pKa = 9.6PFFTQEE209 pKa = 4.98DD210 pKa = 4.61PRR212 pKa = 11.84THH214 pKa = 7.54DD215 pKa = 5.28DD216 pKa = 3.37RR217 pKa = 11.84FKK219 pKa = 11.34DD220 pKa = 3.91YY221 pKa = 11.58VLLLQIDD228 pKa = 3.71TDD230 pKa = 4.01DD231 pKa = 4.94AEE233 pKa = 5.16DD234 pKa = 3.77VQIMWGDD241 pKa = 3.38SGVANFFIHH250 pKa = 7.86PDD252 pKa = 3.41DD253 pKa = 5.13LKK255 pKa = 11.55NKK257 pKa = 9.79DD258 pKa = 3.65FSKK261 pKa = 11.04VLYY264 pKa = 10.62NWDD267 pKa = 3.64CYY269 pKa = 11.2

Molecular weight: 31.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S2FHP4|A0A2S2FHP4_9GAMM Uncharacterized protein OS=Acinetobacter defluvii OX=1871111 GN=DJ533_03700 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.55RR3 pKa = 11.84TFQPSEE9 pKa = 3.97LKK11 pKa = 10.13RR12 pKa = 11.84KK13 pKa = 8.98RR14 pKa = 11.84VHH16 pKa = 6.36GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4AGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.18GRR39 pKa = 11.84HH40 pKa = 5.1SLTVV44 pKa = 3.06

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3434

0

3434

1039734

33

4732

302.8

33.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.456 ± 0.054

0.98 ± 0.016

5.301 ± 0.039

6.082 ± 0.039

4.353 ± 0.035

6.197 ± 0.053

2.386 ± 0.025

6.939 ± 0.035

6.016 ± 0.037

10.213 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.507 ± 0.025

4.74 ± 0.044

3.808 ± 0.034

5.413 ± 0.04

4.212 ± 0.041

6.006 ± 0.035

5.357 ± 0.058

6.542 ± 0.041

1.215 ± 0.015

3.276 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski