Thalassococcus profundi
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4371 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A369TKR5|A0A369TKR5_9RHOB Uncharacterized protein OS=Thalassococcus profundi OX=2282382 GN=DU478_11945 PE=4 SV=1
MM1 pKa = 7.41 KK2 pKa = 9.13 RR3 pKa = 11.84 TLALTAMLGSIATVPAFAAGPTMVEE28 pKa = 4.13 AEE30 pKa = 4.56 PAPMAPAVTPVAPIRR45 pKa = 11.84 DD46 pKa = 3.47 RR47 pKa = 11.84 DD48 pKa = 3.74 FTGFSLGGQFGYY60 pKa = 11.16 GKK62 pKa = 10.46 VDD64 pKa = 3.26 TDD66 pKa = 4.96 SPDD69 pKa = 3.66 LDD71 pKa = 5.43 GGDD74 pKa = 3.46 GLYY77 pKa = 10.96 GLRR80 pKa = 11.84 AYY82 pKa = 10.88 YY83 pKa = 10.43 DD84 pKa = 3.88 YY85 pKa = 11.84 DD86 pKa = 3.76 FGDD89 pKa = 3.85 YY90 pKa = 11.06 VLGGGLQYY98 pKa = 11.29 DD99 pKa = 4.09 EE100 pKa = 5.03 TDD102 pKa = 3.0 IDD104 pKa = 4.42 LDD106 pKa = 3.76 GAATVDD112 pKa = 3.43 SVLRR116 pKa = 11.84 AGVRR120 pKa = 11.84 GGYY123 pKa = 9.93 DD124 pKa = 3.18 YY125 pKa = 11.26 QGNLFYY131 pKa = 10.4 GTAGYY136 pKa = 10.98 AKK138 pKa = 10.64 AFTEE142 pKa = 4.35 DD143 pKa = 3.76 DD144 pKa = 3.74 AVSVGDD150 pKa = 3.68 SNGYY154 pKa = 9.05 FVGVGAEE161 pKa = 4.48 SFITDD166 pKa = 3.25 NVTVGTEE173 pKa = 3.77 LVYY176 pKa = 10.87 HH177 pKa = 7.02 KK178 pKa = 10.64 FQDD181 pKa = 4.0 FEE183 pKa = 5.39 IDD185 pKa = 3.89 DD186 pKa = 4.66 LEE188 pKa = 6.5 ADD190 pKa = 3.44 ATTANIMVNYY200 pKa = 10.12 RR201 pKa = 11.84 FF202 pKa = 3.68
Molecular weight: 21.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.745
IPC_protein 3.77
Toseland 3.541
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.745
Rodwell 3.592
Grimsley 3.439
Solomon 3.77
Lehninger 3.732
Nozaki 3.897
DTASelect 4.177
Thurlkill 3.605
EMBOSS 3.745
Sillero 3.897
Patrickios 0.807
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.8
Protein with the highest isoelectric point:
>tr|A0A369TP77|A0A369TP77_9RHOB IMP dehydrogenase OS=Thalassococcus profundi OX=2282382 GN=DU478_08635 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNLVRR13 pKa = 11.84 KK14 pKa = 8.83 HH15 pKa = 4.64 RR16 pKa = 11.84 HH17 pKa = 3.91 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.37 AGRR29 pKa = 11.84 KK30 pKa = 8.54 ILNARR35 pKa = 11.84 RR36 pKa = 11.84 ARR38 pKa = 11.84 GRR40 pKa = 11.84 KK41 pKa = 9.07 SLSAA45 pKa = 3.93
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4371
0
4371
1404347
25
11755
321.3
34.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.637 ± 0.064
0.872 ± 0.015
6.481 ± 0.064
5.776 ± 0.037
3.65 ± 0.028
8.884 ± 0.056
1.943 ± 0.025
4.858 ± 0.031
2.746 ± 0.042
10.135 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.66 ± 0.029
2.448 ± 0.026
5.183 ± 0.028
3.146 ± 0.019
7.008 ± 0.053
5.087 ± 0.027
5.682 ± 0.047
7.303 ± 0.035
1.369 ± 0.017
2.127 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here