Sphingobium algorifonticola
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3506 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A437JCF2|A0A437JCF2_9SPHN Uncharacterized protein OS=Sphingobium algorifonticola OX=2008318 GN=ENE74_03065 PE=4 SV=1
MM1 pKa = 7.38 RR2 pKa = 11.84 TISSDD7 pKa = 3.33 VTVRR11 pKa = 11.84 LYY13 pKa = 11.25 YY14 pKa = 10.49 LDD16 pKa = 4.45 EE17 pKa = 6.26 GDD19 pKa = 4.0 GAAASTLAYY28 pKa = 10.32 APLSQALALAAAQPEE43 pKa = 4.36 AVQAGLFVQTDD54 pKa = 3.48 NDD56 pKa = 3.78 VIAYY60 pKa = 9.99 LDD62 pKa = 4.89 LIAEE66 pKa = 4.5
Molecular weight: 6.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.823
IPC2_protein 3.656
IPC_protein 3.516
Toseland 3.338
ProMoST 3.694
Dawson 3.554
Bjellqvist 3.846
Wikipedia 3.554
Rodwell 3.376
Grimsley 3.261
Solomon 3.49
Lehninger 3.439
Nozaki 3.706
DTASelect 3.897
Thurlkill 3.439
EMBOSS 3.554
Sillero 3.656
Patrickios 0.477
IPC_peptide 3.478
IPC2_peptide 3.617
IPC2.peptide.svr19 3.741
Protein with the highest isoelectric point:
>tr|A0A437JAG3|A0A437JAG3_9SPHN Mandelate racemase/muconate lactonizing enzyme family protein OS=Sphingobium algorifonticola OX=2008318 GN=ENE74_08810 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 NILRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 SRR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.06 SLSAA44 pKa = 3.93
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.272
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.647
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.369
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.218
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3506
0
3506
1150749
29
6979
328.2
35.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.416 ± 0.061
0.835 ± 0.015
6.276 ± 0.062
4.844 ± 0.044
3.525 ± 0.032
8.876 ± 0.109
2.003 ± 0.029
5.27 ± 0.026
2.84 ± 0.034
9.807 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.558 ± 0.03
2.564 ± 0.037
5.283 ± 0.043
3.211 ± 0.022
7.331 ± 0.058
5.271 ± 0.042
5.37 ± 0.048
7.086 ± 0.03
1.388 ± 0.021
2.245 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here