Rubripirellula reticaptiva
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6084 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C6ETU5|A0A5C6ETU5_9BACT Ankyrin repeat protein OS=Rubripirellula reticaptiva OX=2528013 GN=Poly59_30990 PE=4 SV=1
MM1 pKa = 7.74 KK2 pKa = 10.56 LPIRR6 pKa = 11.84 EE7 pKa = 4.57 RR8 pKa = 11.84 IPEE11 pKa = 4.09 SFVAYY16 pKa = 9.99 LAEE19 pKa = 4.09 VDD21 pKa = 3.63 QLIRR25 pKa = 11.84 CSDD28 pKa = 3.85 PSAITPSDD36 pKa = 4.6 DD37 pKa = 5.75 LLQCDD42 pKa = 3.78 DD43 pKa = 4.48 AYY45 pKa = 10.78 GGRR48 pKa = 11.84 LDD50 pKa = 5.81 DD51 pKa = 4.84 GSLDD55 pKa = 3.51 FAFTFFPEE63 pKa = 4.64 PFDD66 pKa = 5.27 DD67 pKa = 6.27 LPFPPLWYY75 pKa = 8.32 FTLSEE80 pKa = 4.14 DD81 pKa = 4.27 QISKK85 pKa = 10.08 IAAGNLTDD93 pKa = 5.0 LDD95 pKa = 3.81 MWRR98 pKa = 11.84 CPADD102 pKa = 3.39 CGFRR106 pKa = 11.84 GSTPDD111 pKa = 3.83 YY112 pKa = 10.51 YY113 pKa = 10.98 CSRR116 pKa = 11.84 CNN118 pKa = 3.53
Molecular weight: 13.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.716
IPC2_protein 3.872
IPC_protein 3.872
Toseland 3.643
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.846
Rodwell 3.694
Grimsley 3.554
Solomon 3.872
Lehninger 3.821
Nozaki 3.999
DTASelect 4.279
Thurlkill 3.706
EMBOSS 3.846
Sillero 3.999
Patrickios 0.998
IPC_peptide 3.859
IPC2_peptide 3.961
IPC2.peptide.svr19 3.881
Protein with the highest isoelectric point:
>tr|A0A5C6F1Y9|A0A5C6F1Y9_9BACT Uncharacterized protein OS=Rubripirellula reticaptiva OX=2528013 GN=Poly59_15590 PE=4 SV=1
MM1 pKa = 7.42 EE2 pKa = 4.47 FVATGFAKK10 pKa = 10.39 PLRR13 pKa = 11.84 IDD15 pKa = 3.24 SHH17 pKa = 7.81 AHH19 pKa = 6.82 DD20 pKa = 4.15 FQSWSCLPFAANKK33 pKa = 9.12 IGKK36 pKa = 8.77 VDD38 pKa = 3.64 RR39 pKa = 11.84 LGDD42 pKa = 3.52 VDD44 pKa = 4.15 KK45 pKa = 11.51 GQARR49 pKa = 11.84 VSRR52 pKa = 11.84 LAGKK56 pKa = 9.72 RR57 pKa = 11.84 YY58 pKa = 9.27 PILPPRR64 pKa = 11.84 EE65 pKa = 4.25 ASPTTCVAAKK75 pKa = 9.27 RR76 pKa = 11.84 GCPIVPRR83 pKa = 11.84 TIASTGKK90 pKa = 8.07 QTQRR94 pKa = 11.84 PCRR97 pKa = 11.84 STPPPSRR104 pKa = 11.84 AQFSIPHH111 pKa = 4.57 THH113 pKa = 6.41 IKK115 pKa = 9.85 NRR117 pKa = 11.84 PHH119 pKa = 7.47 RR120 pKa = 11.84 SFPPPDD126 pKa = 3.53 KK127 pKa = 10.49 PIPRR131 pKa = 11.84 QAEE134 pKa = 4.27 PYY136 pKa = 8.68 NQSIQTGNFQYY147 pKa = 10.34 TRR149 pKa = 11.84 CTMSLYY155 pKa = 10.54 LRR157 pKa = 11.84 PQEE160 pKa = 4.11 VRR162 pKa = 11.84 SRR164 pKa = 11.84 NHH166 pKa = 6.79 PLPCTHH172 pKa = 7.45
Molecular weight: 19.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.633
IPC_protein 10.335
Toseland 10.657
ProMoST 10.321
Dawson 10.76
Bjellqvist 10.467
Wikipedia 10.95
Rodwell 10.994
Grimsley 10.804
Solomon 10.862
Lehninger 10.833
Nozaki 10.672
DTASelect 10.452
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.687
Patrickios 10.716
IPC_peptide 10.862
IPC2_peptide 9.706
IPC2.peptide.svr19 8.525
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6084
0
6084
2326863
29
12242
382.5
41.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.686 ± 0.037
1.077 ± 0.016
6.605 ± 0.034
5.688 ± 0.031
3.753 ± 0.02
7.765 ± 0.043
2.104 ± 0.017
5.392 ± 0.021
3.691 ± 0.034
9.181 ± 0.033
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.389 ± 0.022
3.436 ± 0.03
4.973 ± 0.025
3.788 ± 0.019
6.333 ± 0.037
7.098 ± 0.033
5.932 ± 0.05
7.349 ± 0.023
1.436 ± 0.015
2.325 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here