Amino acid dipepetide frequency for Escherichia phage JN01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.452AlaAla: 5.452 ± 0.814
0.4AlaCys: 0.4 ± 0.12
4.051AlaAsp: 4.051 ± 0.471
3.901AlaGlu: 3.901 ± 0.457
2.451AlaPhe: 2.451 ± 0.34
5.302AlaGly: 5.302 ± 0.556
1.6AlaHis: 1.6 ± 0.336
4.501AlaIle: 4.501 ± 0.437
4.701AlaLys: 4.701 ± 0.492
5.852AlaLeu: 5.852 ± 0.667
2.451AlaMet: 2.451 ± 0.372
3.401AlaAsn: 3.401 ± 0.588
1.751AlaPro: 1.751 ± 0.352
2.601AlaGln: 2.601 ± 0.369
2.701AlaArg: 2.701 ± 0.45
4.451AlaSer: 4.451 ± 0.6
4.851AlaThr: 4.851 ± 0.567
4.801AlaVal: 4.801 ± 0.525
1.05AlaTrp: 1.05 ± 0.245
3.051AlaTyr: 3.051 ± 0.401
0.0AlaXaa: 0.0 ± 0.0
Cys
0.25CysAla: 0.25 ± 0.142
0.15CysCys: 0.15 ± 0.076
0.65CysAsp: 0.65 ± 0.188
0.7CysGlu: 0.7 ± 0.192
0.85CysPhe: 0.85 ± 0.167
0.75CysGly: 0.75 ± 0.245
0.45CysHis: 0.45 ± 0.167
0.55CysIle: 0.55 ± 0.149
1.35CysLys: 1.35 ± 0.259
1.0CysLeu: 1.0 ± 0.187
0.25CysMet: 0.25 ± 0.116
0.75CysAsn: 0.75 ± 0.154
0.55CysPro: 0.55 ± 0.169
0.3CysGln: 0.3 ± 0.123
0.6CysArg: 0.6 ± 0.179
1.45CysSer: 1.45 ± 0.317
0.75CysThr: 0.75 ± 0.202
1.0CysVal: 1.0 ± 0.233
0.1CysTrp: 0.1 ± 0.072
0.65CysTyr: 0.65 ± 0.158
0.0CysXaa: 0.0 ± 0.0
Asp
4.351AspAla: 4.351 ± 0.432
0.55AspCys: 0.55 ± 0.193
3.501AspAsp: 3.501 ± 0.448
4.451AspGlu: 4.451 ± 0.535
3.501AspPhe: 3.501 ± 0.398
4.801AspGly: 4.801 ± 0.422
0.45AspHis: 0.45 ± 0.192
4.101AspIle: 4.101 ± 0.468
3.901AspLys: 3.901 ± 0.432
5.252AspLeu: 5.252 ± 0.466
1.35AspMet: 1.35 ± 0.287
3.351AspAsn: 3.351 ± 0.432
1.3AspPro: 1.3 ± 0.328
0.7AspGln: 0.7 ± 0.178
1.951AspArg: 1.951 ± 0.243
4.051AspSer: 4.051 ± 0.428
3.551AspThr: 3.551 ± 0.345
5.152AspVal: 5.152 ± 0.529
1.4AspTrp: 1.4 ± 0.271
2.551AspTyr: 2.551 ± 0.312
0.0AspXaa: 0.0 ± 0.0
Glu
5.652GluAla: 5.652 ± 0.708
0.75GluCys: 0.75 ± 0.158
4.151GluAsp: 4.151 ± 0.422
5.202GluGlu: 5.202 ± 0.641
3.051GluPhe: 3.051 ± 0.408
4.201GluGly: 4.201 ± 0.469
1.35GluHis: 1.35 ± 0.263
3.851GluIle: 3.851 ± 0.464
4.751GluLys: 4.751 ± 0.593
5.502GluLeu: 5.502 ± 0.525
2.251GluMet: 2.251 ± 0.321
3.601GluAsn: 3.601 ± 0.433
1.751GluPro: 1.751 ± 0.264
2.151GluGln: 2.151 ± 0.324
2.401GluArg: 2.401 ± 0.32
3.401GluSer: 3.401 ± 0.371
3.301GluThr: 3.301 ± 0.306
4.451GluVal: 4.451 ± 0.439
0.55GluTrp: 0.55 ± 0.159
2.351GluTyr: 2.351 ± 0.322
0.0GluXaa: 0.0 ± 0.0
Phe
2.451PheAla: 2.451 ± 0.443
0.65PheCys: 0.65 ± 0.188
3.401PheAsp: 3.401 ± 0.342
3.101PheGlu: 3.101 ± 0.414
1.6PhePhe: 1.6 ± 0.314
2.351PheGly: 2.351 ± 0.366
0.8PheHis: 0.8 ± 0.189
2.751PheIle: 2.751 ± 0.352
3.951PheLys: 3.951 ± 0.422
2.851PheLeu: 2.851 ± 0.394
0.9PheMet: 0.9 ± 0.212
2.651PheAsn: 2.651 ± 0.323
1.1PhePro: 1.1 ± 0.239
1.55PheGln: 1.55 ± 0.24
1.801PheArg: 1.801 ± 0.284
3.551PheSer: 3.551 ± 0.392
2.401PheThr: 2.401 ± 0.32
2.901PheVal: 2.901 ± 0.374
0.3PheTrp: 0.3 ± 0.105
1.951PheTyr: 1.951 ± 0.337
0.0PheXaa: 0.0 ± 0.0
Gly
5.202GlyAla: 5.202 ± 0.496
1.05GlyCys: 1.05 ± 0.25
3.251GlyAsp: 3.251 ± 0.367
4.201GlyGlu: 4.201 ± 0.61
3.001GlyPhe: 3.001 ± 0.445
4.451GlyGly: 4.451 ± 0.58
1.2GlyHis: 1.2 ± 0.237
4.451GlyIle: 4.451 ± 0.478
5.852GlyLys: 5.852 ± 0.577
4.401GlyLeu: 4.401 ± 0.573
0.85GlyMet: 0.85 ± 0.225
3.401GlyAsn: 3.401 ± 0.421
0.1GlyPro: 0.1 ± 0.066
2.301GlyGln: 2.301 ± 0.417
2.551GlyArg: 2.551 ± 0.313
4.451GlySer: 4.451 ± 0.576
4.001GlyThr: 4.001 ± 0.566
5.452GlyVal: 5.452 ± 0.46
0.75GlyTrp: 0.75 ± 0.209
2.851GlyTyr: 2.851 ± 0.317
0.0GlyXaa: 0.0 ± 0.0
His
1.15HisAla: 1.15 ± 0.219
0.35HisCys: 0.35 ± 0.136
0.8HisAsp: 0.8 ± 0.15
1.0HisGlu: 1.0 ± 0.249
1.0HisPhe: 1.0 ± 0.22
0.95HisGly: 0.95 ± 0.206
0.45HisHis: 0.45 ± 0.163
1.15HisIle: 1.15 ± 0.25
1.05HisLys: 1.05 ± 0.286
1.901HisLeu: 1.901 ± 0.298
0.55HisMet: 0.55 ± 0.161
1.15HisAsn: 1.15 ± 0.222
0.75HisPro: 0.75 ± 0.201
0.4HisGln: 0.4 ± 0.115
0.95HisArg: 0.95 ± 0.177
1.35HisSer: 1.35 ± 0.361
1.751HisThr: 1.751 ± 0.528
1.4HisVal: 1.4 ± 0.313
0.15HisTrp: 0.15 ± 0.071
1.0HisTyr: 1.0 ± 0.218
0.0HisXaa: 0.0 ± 0.0
Ile
3.801IleAla: 3.801 ± 0.44
0.85IleCys: 0.85 ± 0.197
3.801IleAsp: 3.801 ± 0.392
3.901IleGlu: 3.901 ± 0.439
2.601IlePhe: 2.601 ± 0.295
3.401IleGly: 3.401 ± 0.422
1.1IleHis: 1.1 ± 0.227
4.151IleIle: 4.151 ± 0.467
4.751IleLys: 4.751 ± 0.608
3.901IleLeu: 3.901 ± 0.429
1.701IleMet: 1.701 ± 0.375
3.551IleAsn: 3.551 ± 0.425
2.701IlePro: 2.701 ± 0.373
1.901IleGln: 1.901 ± 0.291
2.951IleArg: 2.951 ± 0.419
4.201IleSer: 4.201 ± 0.486
4.851IleThr: 4.851 ± 0.473
3.651IleVal: 3.651 ± 0.53
0.6IleTrp: 0.6 ± 0.173
3.051IleTyr: 3.051 ± 0.355
0.0IleXaa: 0.0 ± 0.0
Lys
5.452LysAla: 5.452 ± 0.541
0.9LysCys: 0.9 ± 0.195
4.801LysAsp: 4.801 ± 0.582
5.252LysGlu: 5.252 ± 0.604
2.651LysPhe: 2.651 ± 0.36
5.002LysGly: 5.002 ± 0.471
1.0LysHis: 1.0 ± 0.212
4.451LysIle: 4.451 ± 0.435
5.202LysLys: 5.202 ± 0.721
6.402LysLeu: 6.402 ± 0.547
2.701LysMet: 2.701 ± 0.39
4.251LysAsn: 4.251 ± 0.388
2.201LysPro: 2.201 ± 0.324
2.351LysGln: 2.351 ± 0.322
2.901LysArg: 2.901 ± 0.269
4.401LysSer: 4.401 ± 0.472
5.202LysThr: 5.202 ± 0.59
6.952LysVal: 6.952 ± 0.797
0.45LysTrp: 0.45 ± 0.104
2.451LysTyr: 2.451 ± 0.365
0.0LysXaa: 0.0 ± 0.0
Leu
5.652LeuAla: 5.652 ± 0.556
1.05LeuCys: 1.05 ± 0.24
5.452LeuAsp: 5.452 ± 0.494
5.902LeuGlu: 5.902 ± 0.521
2.951LeuPhe: 2.951 ± 0.38
4.051LeuGly: 4.051 ± 0.415
1.701LeuHis: 1.701 ± 0.343
3.551LeuIle: 3.551 ± 0.36
6.202LeuLys: 6.202 ± 0.585
6.652LeuLeu: 6.652 ± 0.516
2.551LeuMet: 2.551 ± 0.363
4.501LeuAsn: 4.501 ± 0.457
3.701LeuPro: 3.701 ± 0.458
3.701LeuGln: 3.701 ± 0.454
3.951LeuArg: 3.951 ± 0.425
5.902LeuSer: 5.902 ± 0.507
5.402LeuThr: 5.402 ± 0.5
4.401LeuVal: 4.401 ± 0.514
0.7LeuTrp: 0.7 ± 0.179
2.651LeuTyr: 2.651 ± 0.472
0.0LeuXaa: 0.0 ± 0.0
Met
2.101MetAla: 2.101 ± 0.334
0.3MetCys: 0.3 ± 0.11
1.0MetAsp: 1.0 ± 0.209
1.701MetGlu: 1.701 ± 0.212
1.25MetPhe: 1.25 ± 0.325
1.55MetGly: 1.55 ± 0.244
0.3MetHis: 0.3 ± 0.115
1.35MetIle: 1.35 ± 0.258
2.601MetLys: 2.601 ± 0.401
2.301MetLeu: 2.301 ± 0.398
0.75MetMet: 0.75 ± 0.225
1.35MetAsn: 1.35 ± 0.227
0.55MetPro: 0.55 ± 0.161
1.901MetGln: 1.901 ± 0.36
1.6MetArg: 1.6 ± 0.275
1.951MetSer: 1.951 ± 0.299
1.901MetThr: 1.901 ± 0.3
1.35MetVal: 1.35 ± 0.25
0.3MetTrp: 0.3 ± 0.123
0.75MetTyr: 0.75 ± 0.211
0.0MetXaa: 0.0 ± 0.0
Asn
3.951AsnAla: 3.951 ± 0.516
0.55AsnCys: 0.55 ± 0.187
2.951AsnAsp: 2.951 ± 0.417
2.501AsnGlu: 2.501 ± 0.316
2.651AsnPhe: 2.651 ± 0.32
4.551AsnGly: 4.551 ± 0.585
1.55AsnHis: 1.55 ± 0.316
3.701AsnIle: 3.701 ± 0.432
3.901AsnLys: 3.901 ± 0.522
5.052AsnLeu: 5.052 ± 0.539
1.15AsnMet: 1.15 ± 0.298
3.651AsnAsn: 3.651 ± 0.455
2.301AsnPro: 2.301 ± 0.357
1.751AsnGln: 1.751 ± 0.337
2.101AsnArg: 2.101 ± 0.326
4.101AsnSer: 4.101 ± 0.491
2.601AsnThr: 2.601 ± 0.493
3.701AsnVal: 3.701 ± 0.387
0.7AsnTrp: 0.7 ± 0.17
2.201AsnTyr: 2.201 ± 0.307
0.0AsnXaa: 0.0 ± 0.0
Pro
1.65ProAla: 1.65 ± 0.264
0.4ProCys: 0.4 ± 0.137
2.051ProAsp: 2.051 ± 0.321
2.801ProGlu: 2.801 ± 0.362
1.751ProPhe: 1.751 ± 0.311
0.2ProGly: 0.2 ± 0.098
0.65ProHis: 0.65 ± 0.262
1.55ProIle: 1.55 ± 0.254
2.251ProLys: 2.251 ± 0.348
2.301ProLeu: 2.301 ± 0.335
0.9ProMet: 0.9 ± 0.225
1.65ProAsn: 1.65 ± 0.256
1.05ProPro: 1.05 ± 0.265
1.2ProGln: 1.2 ± 0.257
1.1ProArg: 1.1 ± 0.3
2.651ProSer: 2.651 ± 0.43
2.751ProThr: 2.751 ± 0.363
2.201ProVal: 2.201 ± 0.308
0.2ProTrp: 0.2 ± 0.093
1.1ProTyr: 1.1 ± 0.227
0.0ProXaa: 0.0 ± 0.0
Gln
2.351GlnAla: 2.351 ± 0.341
0.4GlnCys: 0.4 ± 0.127
1.45GlnAsp: 1.45 ± 0.296
2.601GlnGlu: 2.601 ± 0.396
1.15GlnPhe: 1.15 ± 0.203
1.901GlnGly: 1.901 ± 0.259
0.5GlnHis: 0.5 ± 0.141
2.951GlnIle: 2.951 ± 0.384
2.451GlnLys: 2.451 ± 0.378
3.201GlnLeu: 3.201 ± 0.461
1.2GlnMet: 1.2 ± 0.293
1.3GlnAsn: 1.3 ± 0.251
1.4GlnPro: 1.4 ± 0.267
1.35GlnGln: 1.35 ± 0.253
2.001GlnArg: 2.001 ± 0.296
2.151GlnSer: 2.151 ± 0.309
1.901GlnThr: 1.901 ± 0.282
3.001GlnVal: 3.001 ± 0.376
0.5GlnTrp: 0.5 ± 0.166
1.901GlnTyr: 1.901 ± 0.266
0.0GlnXaa: 0.0 ± 0.0
Arg
2.401ArgAla: 2.401 ± 0.345
1.0ArgCys: 1.0 ± 0.249
2.951ArgAsp: 2.951 ± 0.426
2.101ArgGlu: 2.101 ± 0.373
1.5ArgPhe: 1.5 ± 0.302
2.501ArgGly: 2.501 ± 0.436
1.15ArgHis: 1.15 ± 0.24
2.801ArgIle: 2.801 ± 0.301
2.701ArgLys: 2.701 ± 0.437
3.551ArgLeu: 3.551 ± 0.45
1.35ArgMet: 1.35 ± 0.255
2.301ArgAsn: 2.301 ± 0.286
0.95ArgPro: 0.95 ± 0.202
1.65ArgGln: 1.65 ± 0.316
2.251ArgArg: 2.251 ± 0.324
2.251ArgSer: 2.251 ± 0.312
2.701ArgThr: 2.701 ± 0.419
2.751ArgVal: 2.751 ± 0.388
0.7ArgTrp: 0.7 ± 0.239
2.151ArgTyr: 2.151 ± 0.337
0.0ArgXaa: 0.0 ± 0.0
Ser
4.551SerAla: 4.551 ± 0.551
0.8SerCys: 0.8 ± 0.213
4.601SerAsp: 4.601 ± 0.564
4.551SerGlu: 4.551 ± 0.427
2.851SerPhe: 2.851 ± 0.407
3.701SerGly: 3.701 ± 0.478
1.55SerHis: 1.55 ± 0.299
3.801SerIle: 3.801 ± 0.422
4.151SerLys: 4.151 ± 0.445
6.252SerLeu: 6.252 ± 0.506
1.6SerMet: 1.6 ± 0.252
3.501SerAsn: 3.501 ± 0.43
1.901SerPro: 1.901 ± 0.331
3.151SerGln: 3.151 ± 0.346
2.751SerArg: 2.751 ± 0.398
5.252SerSer: 5.252 ± 0.625
3.851SerThr: 3.851 ± 0.515
5.602SerVal: 5.602 ± 0.538
0.85SerTrp: 0.85 ± 0.172
3.401SerTyr: 3.401 ± 0.477
0.0SerXaa: 0.0 ± 0.0
Thr
4.251ThrAla: 4.251 ± 0.539
0.8ThrCys: 0.8 ± 0.203
3.601ThrAsp: 3.601 ± 0.401
3.301ThrGlu: 3.301 ± 0.355
3.051ThrPhe: 3.051 ± 0.385
5.452ThrGly: 5.452 ± 0.671
1.35ThrHis: 1.35 ± 0.324
4.201ThrIle: 4.201 ± 0.498
4.301ThrLys: 4.301 ± 0.576
5.202ThrLeu: 5.202 ± 0.462
0.9ThrMet: 0.9 ± 0.218
2.801ThrAsn: 2.801 ± 0.413
2.251ThrPro: 2.251 ± 0.335
2.651ThrGln: 2.651 ± 0.388
2.651ThrArg: 2.651 ± 0.327
4.951ThrSer: 4.951 ± 0.515
4.601ThrThr: 4.601 ± 0.692
5.652ThrVal: 5.652 ± 0.618
0.6ThrTrp: 0.6 ± 0.199
2.951ThrTyr: 2.951 ± 0.335
0.0ThrXaa: 0.0 ± 0.0
Val
5.752ValAla: 5.752 ± 0.582
1.1ValCys: 1.1 ± 0.194
4.501ValAsp: 4.501 ± 0.411
4.351ValGlu: 4.351 ± 0.633
2.501ValPhe: 2.501 ± 0.339
4.851ValGly: 4.851 ± 0.577
1.2ValHis: 1.2 ± 0.247
4.751ValIle: 4.751 ± 0.559
6.552ValLys: 6.552 ± 0.562
4.501ValLeu: 4.501 ± 0.472
2.001ValMet: 2.001 ± 0.337
5.152ValAsn: 5.152 ± 0.519
2.201ValPro: 2.201 ± 0.355
2.051ValGln: 2.051 ± 0.394
2.701ValArg: 2.701 ± 0.403
5.202ValSer: 5.202 ± 0.491
4.901ValThr: 4.901 ± 0.625
4.851ValVal: 4.851 ± 0.498
0.6ValTrp: 0.6 ± 0.188
2.601ValTyr: 2.601 ± 0.353
0.0ValXaa: 0.0 ± 0.0
Trp
0.45TrpAla: 0.45 ± 0.161
0.2TrpCys: 0.2 ± 0.093
0.65TrpAsp: 0.65 ± 0.175
0.6TrpGlu: 0.6 ± 0.15
0.6TrpPhe: 0.6 ± 0.153
0.75TrpGly: 0.75 ± 0.177
0.05TrpHis: 0.05 ± 0.046
0.55TrpIle: 0.55 ± 0.157
1.1TrpLys: 1.1 ± 0.204
1.2TrpLeu: 1.2 ± 0.241
0.35TrpMet: 0.35 ± 0.141
0.95TrpAsn: 0.95 ± 0.216
0.3TrpPro: 0.3 ± 0.12
0.35TrpGln: 0.35 ± 0.124
0.35TrpArg: 0.35 ± 0.138
0.45TrpSer: 0.45 ± 0.114
0.7TrpThr: 0.7 ± 0.197
0.6TrpVal: 0.6 ± 0.157
0.3TrpTrp: 0.3 ± 0.127
0.7TrpTyr: 0.7 ± 0.157
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.451TyrAla: 2.451 ± 0.327
0.85TyrCys: 0.85 ± 0.162
2.601TyrAsp: 2.601 ± 0.339
2.601TyrGlu: 2.601 ± 0.333
2.051TyrPhe: 2.051 ± 0.28
3.051TyrGly: 3.051 ± 0.349
0.85TyrHis: 0.85 ± 0.183
2.251TyrIle: 2.251 ± 0.329
3.251TyrLys: 3.251 ± 0.313
3.351TyrLeu: 3.351 ± 0.391
1.05TyrMet: 1.05 ± 0.232
2.351TyrAsn: 2.351 ± 0.359
1.6TyrPro: 1.6 ± 0.229
1.65TyrGln: 1.65 ± 0.309
1.45TyrArg: 1.45 ± 0.225
2.501TyrSer: 2.501 ± 0.326
3.551TyrThr: 3.551 ± 0.405
2.451TyrVal: 2.451 ± 0.319
0.4TyrTrp: 0.4 ± 0.126
1.35TyrTyr: 1.35 ± 0.286
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 97 proteins (19995 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski