Spraguea lophii (strain 42_110) (Microsporidian parasite)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Microsporidia; Apansporoblastina; Spraguidae; Spraguea; Spraguea lophii

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2499 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S7XIE2|S7XIE2_SPRLO Anaphase promoting complex subunit (Cdc27/Nuc2/BimAfamily) OS=Spraguea lophii (strain 42_110) OX=1358809 GN=SLOPH_2165 PE=4 SV=1
MM1 pKa = 7.31SGNYY5 pKa = 9.76KK6 pKa = 9.93IEE8 pKa = 4.05TNEE11 pKa = 3.62NFNYY15 pKa = 10.57GDD17 pKa = 4.61LEE19 pKa = 4.42FQFSSYY25 pKa = 11.26KK26 pKa = 10.45NFYY29 pKa = 10.9DD30 pKa = 3.72EE31 pKa = 5.33VDD33 pKa = 3.36IQSFEE38 pKa = 4.26YY39 pKa = 10.38EE40 pKa = 3.59DD41 pKa = 4.55GYY43 pKa = 11.41FYY45 pKa = 10.38FPCPCGDD52 pKa = 3.27RR53 pKa = 11.84FEE55 pKa = 4.77VSLEE59 pKa = 3.81EE60 pKa = 4.34LKK62 pKa = 11.14DD63 pKa = 3.57GEE65 pKa = 4.87EE66 pKa = 4.07IATCPSCSLIVKK78 pKa = 9.93IIYY81 pKa = 10.33EE82 pKa = 4.23EE83 pKa = 5.01DD84 pKa = 3.42DD85 pKa = 3.64LLEE88 pKa = 4.16FEE90 pKa = 4.94KK91 pKa = 11.13

Molecular weight: 10.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S7W532|S7W532_SPRLO Uncharacterized protein (Fragment) OS=Spraguea lophii (strain 42_110) OX=1358809 GN=SLOPH_615 PE=4 SV=1
MM1 pKa = 7.5CVFLEE6 pKa = 3.92VDD8 pKa = 3.29EE9 pKa = 4.71TVISRR14 pKa = 11.84RR15 pKa = 11.84GIIINPTTLPDD26 pKa = 5.28GITNTMWILGVVNQTNIRR44 pKa = 11.84NFFIKK49 pKa = 10.16RR50 pKa = 11.84VRR52 pKa = 11.84NRR54 pKa = 11.84RR55 pKa = 11.84SNTLTRR61 pKa = 11.84VLEE64 pKa = 4.03GVIRR68 pKa = 11.84VGSVLCNHH76 pKa = 7.66GYY78 pKa = 9.8PSYY81 pKa = 10.45PQLLKK86 pKa = 10.17TT87 pKa = 4.25

Molecular weight: 9.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2499

0

2499

844153

50

3114

337.8

39.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.64 ± 0.037

1.601 ± 0.021

5.802 ± 0.039

7.663 ± 0.055

5.066 ± 0.042

3.593 ± 0.039

1.673 ± 0.017

11.157 ± 0.055

10.596 ± 0.061

9.016 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.211 ± 0.017

9.449 ± 0.1

2.402 ± 0.029

2.366 ± 0.024

3.457 ± 0.03

6.472 ± 0.034

4.869 ± 0.033

4.417 ± 0.038

0.452 ± 0.01

5.082 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski