Amino acid dipepetide frequency for Spraguea lophii (strain 42_110) (Microsporidian parasite)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	1.08AlaAla: 1.08 ± 0.05	0.377AlaCys: 0.377 ± 0.023	1.309AlaAsp: 1.309 ± 0.043	1.923AlaGlu: 1.923 ± 0.057	1.305AlaPhe: 1.305 ± 0.043	1.17AlaGly: 1.17 ± 0.043	0.509AlaHis: 0.509 ± 0.024	2.885AlaIle: 2.885 ± 0.064	2.232AlaLys: 2.232 ± 0.064	2.92AlaLeu: 2.92 ± 0.063	0.739AlaMet: 0.739 ± 0.033	1.604AlaAsn: 1.604 ± 0.048	0.707AlaPro: 0.707 ± 0.036	0.73AlaGln: 0.73 ± 0.032	0.923AlaArg: 0.923 ± 0.035	1.648AlaSer: 1.648 ± 0.045	1.316AlaThr: 1.316 ± 0.043	1.51AlaVal: 1.51 ± 0.048	0.133AlaTrp: 0.133 ± 0.012	1.38AlaTyr: 1.38 ± 0.041	0.002AlaXaa: 0.002 ± 0.001
Cys	0.492CysAla: 0.492 ± 0.024	0.378CysCys: 0.378 ± 0.022	0.993CysAsp: 0.993 ± 0.04	0.856CysGlu: 0.856 ± 0.031	0.955CysPhe: 0.955 ± 0.03	0.883CysGly: 0.883 ± 0.038	0.219CysHis: 0.219 ± 0.019	1.673CysIle: 1.673 ± 0.045	1.599CysLys: 1.599 ± 0.045	1.404CysLeu: 1.404 ± 0.041	0.319CysMet: 0.319 ± 0.021	1.393CysAsn: 1.393 ± 0.044	0.404CysPro: 0.404 ± 0.024	0.257CysGln: 0.257 ± 0.017	0.625CysArg: 0.625 ± 0.025	1.156CysSer: 1.156 ± 0.039	0.72CysThr: 0.72 ± 0.029	0.737CysVal: 0.737 ± 0.035	0.072CysTrp: 0.072 ± 0.008	0.871CysTyr: 0.871 ± 0.036	0.002CysXaa: 0.002 ± 0.002
Asp	1.303AspAla: 1.303 ± 0.042	0.887AspCys: 0.887 ± 0.034	3.649AspAsp: 3.649 ± 0.083	4.37AspGlu: 4.37 ± 0.09	2.734AspPhe: 2.734 ± 0.058	2.558AspGly: 2.558 ± 0.063	0.705AspHis: 0.705 ± 0.028	7.556AspIle: 7.556 ± 0.116	6.198AspLys: 6.198 ± 0.113	4.519AspLeu: 4.519 ± 0.09	1.317AspMet: 1.317 ± 0.042	6.505AspAsn: 6.505 ± 0.147	1.116AspPro: 1.116 ± 0.039	0.99AspGln: 0.99 ± 0.041	2.017AspArg: 2.017 ± 0.063	3.44AspSer: 3.44 ± 0.072	2.523AspThr: 2.523 ± 0.056	2.771AspVal: 2.771 ± 0.058	0.27AspTrp: 0.27 ± 0.02	2.588AspTyr: 2.588 ± 0.062	0.006AspXaa: 0.006 ± 0.003
Glu	1.982GluAla: 1.982 ± 0.06	1.271GluCys: 1.271 ± 0.041	4.657GluAsp: 4.657 ± 0.088	8.231GluGlu: 8.231 ± 0.169	2.999GluPhe: 2.999 ± 0.062	2.276GluGly: 2.276 ± 0.052	1.142GluHis: 1.142 ± 0.034	9.169GluIle: 9.169 ± 0.145	8.343GluLys: 8.343 ± 0.136	6.031GluLeu: 6.031 ± 0.104	1.829GluMet: 1.829 ± 0.044	8.117GluAsn: 8.117 ± 0.12	1.111GluPro: 1.111 ± 0.039	1.93GluGln: 1.93 ± 0.058	2.757GluArg: 2.757 ± 0.061	4.024GluSer: 4.024 ± 0.091	2.87GluThr: 2.87 ± 0.067	2.991GluVal: 2.991 ± 0.068	0.313GluTrp: 0.313 ± 0.02	4.58GluTyr: 4.58 ± 0.084	0.011GluXaa: 0.011 ± 0.004
Phe	1.234PheAla: 1.234 ± 0.043	0.988PheCys: 0.988 ± 0.036	2.52PheAsp: 2.52 ± 0.054	2.731PheGlu: 2.731 ± 0.072	2.998PhePhe: 2.998 ± 0.066	1.752PheGly: 1.752 ± 0.046	0.913PheHis: 0.913 ± 0.036	6.055PheIle: 6.055 ± 0.092	4.698PheLys: 4.698 ± 0.071	5.693PheLeu: 5.693 ± 0.101	1.067PheMet: 1.067 ± 0.04	3.851PheAsn: 3.851 ± 0.071	1.313PhePro: 1.313 ± 0.042	1.144PheGln: 1.144 ± 0.04	1.45PheArg: 1.45 ± 0.041	3.781PheSer: 3.781 ± 0.067	2.456PheThr: 2.456 ± 0.059	2.342PheVal: 2.342 ± 0.058	0.232PheTrp: 0.232 ± 0.019	3.428PheTyr: 3.428 ± 0.075	0.009PheXaa: 0.009 ± 0.004
Gly	1.127GlyAla: 1.127 ± 0.041	0.768GlyCys: 0.768 ± 0.029	2.116GlyAsp: 2.116 ± 0.06	2.357GlyGlu: 2.357 ± 0.054	1.916GlyPhe: 1.916 ± 0.052	1.637GlyGly: 1.637 ± 0.065	0.602GlyHis: 0.602 ± 0.031	4.173GlyIle: 4.173 ± 0.072	3.528GlyLys: 3.528 ± 0.075	2.875GlyLeu: 2.875 ± 0.063	0.993GlyMet: 0.993 ± 0.035	2.827GlyAsn: 2.827 ± 0.066	0.64GlyPro: 0.64 ± 0.032	0.732GlyGln: 0.732 ± 0.03	1.488GlyArg: 1.488 ± 0.049	2.245GlySer: 2.245 ± 0.059	1.623GlyThr: 1.623 ± 0.052	2.04GlyVal: 2.04 ± 0.055	0.217GlyTrp: 0.217 ± 0.017	2.026GlyTyr: 2.026 ± 0.05	0.006GlyXaa: 0.006 ± 0.002
His	0.423HisAla: 0.423 ± 0.024	0.294HisCys: 0.294 ± 0.019	0.752HisAsp: 0.752 ± 0.032	0.891HisGlu: 0.891 ± 0.035	0.804HisPhe: 0.804 ± 0.035	0.693HisGly: 0.693 ± 0.03	0.391HisHis: 0.391 ± 0.026	1.839HisIle: 1.839 ± 0.056	1.583HisLys: 1.583 ± 0.055	1.574HisLeu: 1.574 ± 0.046	0.338HisMet: 0.338 ± 0.02	1.527HisAsn: 1.527 ± 0.046	0.612HisPro: 0.612 ± 0.028	0.466HisGln: 0.466 ± 0.025	0.717HisArg: 0.717 ± 0.032	1.238HisSer: 1.238 ± 0.042	1.038HisThr: 1.038 ± 0.036	0.681HisVal: 0.681 ± 0.029	0.101HisTrp: 0.101 ± 0.011	0.759HisTyr: 0.759 ± 0.03	0.006HisXaa: 0.006 ± 0.003
Ile	2.902IleAla: 2.902 ± 0.056	1.703IleCys: 1.703 ± 0.049	6.352IleAsp: 6.352 ± 0.098	8.891IleGlu: 8.891 ± 0.113	6.717IlePhe: 6.717 ± 0.108	3.591IleGly: 3.591 ± 0.078	1.859IleHis: 1.859 ± 0.055	11.56IleIle: 11.56 ± 0.163	12.268IleLys: 12.268 ± 0.166	11.077IleLeu: 11.077 ± 0.157	2.171IleMet: 2.171 ± 0.054	9.638IleAsn: 9.638 ± 0.145	3.219IlePro: 3.219 ± 0.061	2.671IleGln: 2.671 ± 0.062	3.317IleArg: 3.317 ± 0.06	7.994IleSer: 7.994 ± 0.121	5.557IleThr: 5.557 ± 0.094	4.485IleVal: 4.485 ± 0.071	0.404IleTrp: 0.404 ± 0.021	5.177IleTyr: 5.177 ± 0.084	0.018IleXaa: 0.018 ± 0.005
Lys	2.309LysAla: 2.309 ± 0.051	1.482LysCys: 1.482 ± 0.05	6.839LysAsp: 6.839 ± 0.114	10.266LysGlu: 10.266 ± 0.15	3.653LysPhe: 3.653 ± 0.069	3.061LysGly: 3.061 ± 0.072	1.834LysHis: 1.834 ± 0.05	12.937LysIle: 12.937 ± 0.152	12.019LysLys: 12.019 ± 0.193	7.886LysLeu: 7.886 ± 0.111	2.567LysMet: 2.567 ± 0.056	11.383LysAsn: 11.383 ± 0.144	2.135LysPro: 2.135 ± 0.058	2.601LysGln: 2.601 ± 0.061	4.034LysArg: 4.034 ± 0.066	5.724LysSer: 5.724 ± 0.089	4.59LysThr: 4.59 ± 0.084	3.947LysVal: 3.947 ± 0.083	0.434LysTrp: 0.434 ± 0.022	6.251LysTyr: 6.251 ± 0.095	0.012LysXaa: 0.012 ± 0.004
Leu	2.487LeuAla: 2.487 ± 0.07	1.658LeuCys: 1.658 ± 0.045	5.099LeuAsp: 5.099 ± 0.085	7.24LeuGlu: 7.24 ± 0.112	5.282LeuPhe: 5.282 ± 0.116	3.17LeuGly: 3.17 ± 0.062	1.586LeuHis: 1.586 ± 0.043	7.705LeuIle: 7.705 ± 0.134	10.311LeuLys: 10.311 ± 0.169	8.703LeuLeu: 8.703 ± 0.126	1.667LeuMet: 1.667 ± 0.042	6.86LeuAsn: 6.86 ± 0.098	2.644LeuPro: 2.644 ± 0.057	2.522LeuGln: 2.522 ± 0.063	3.265LeuArg: 3.265 ± 0.07	7.082LeuSer: 7.082 ± 0.112	4.034LeuThr: 4.034 ± 0.074	3.713LeuVal: 3.713 ± 0.066	0.477LeuTrp: 0.477 ± 0.027	4.638LeuTyr: 4.638 ± 0.073	0.015LeuXaa: 0.015 ± 0.005
Met	0.785MetAla: 0.785 ± 0.032	0.36MetCys: 0.36 ± 0.022	1.47MetAsp: 1.47 ± 0.044	1.668MetGlu: 1.668 ± 0.044	1.125MetPhe: 1.125 ± 0.041	0.839MetGly: 0.839 ± 0.035	0.39MetHis: 0.39 ± 0.023	2.078MetIle: 2.078 ± 0.053	2.513MetLys: 2.513 ± 0.055	2.157MetLeu: 2.157 ± 0.053	0.461MetMet: 0.461 ± 0.026	1.953MetAsn: 1.953 ± 0.049	0.454MetPro: 0.454 ± 0.025	0.53MetGln: 0.53 ± 0.024	0.71MetArg: 0.71 ± 0.028	1.307MetSer: 1.307 ± 0.036	0.813MetThr: 0.813 ± 0.034	1.474MetVal: 1.474 ± 0.046	0.11MetTrp: 0.11 ± 0.011	0.91MetTyr: 0.91 ± 0.033	0.004MetXaa: 0.004 ± 0.002
Asn	1.82AsnAla: 1.82 ± 0.047	1.153AsnCys: 1.153 ± 0.035	5.472AsnAsp: 5.472 ± 0.123	6.162AsnGlu: 6.162 ± 0.116	3.854AsnPhe: 3.854 ± 0.071	3.049AsnGly: 3.049 ± 0.068	1.561AsnHis: 1.561 ± 0.055	13.288AsnIle: 13.288 ± 0.169	10.75AsnLys: 10.75 ± 0.159	7.223AsnLeu: 7.223 ± 0.136	2.027AsnMet: 2.027 ± 0.05	12.829AsnAsn: 12.829 ± 0.324	1.916AsnPro: 1.916 ± 0.058	1.798AsnGln: 1.798 ± 0.056	3.101AsnArg: 3.101 ± 0.073	5.109AsnSer: 5.109 ± 0.095	5.612AsnThr: 5.612 ± 0.114	3.563AsnVal: 3.563 ± 0.068	0.302AsnTrp: 0.302 ± 0.018	3.88AsnTyr: 3.88 ± 0.084	0.02AsnXaa: 0.02 ± 0.005
Pro	0.64ProAla: 0.64 ± 0.032	0.362ProCys: 0.362 ± 0.027	1.161ProAsp: 1.161 ± 0.038	1.874ProGlu: 1.874 ± 0.057	1.327ProPhe: 1.327 ± 0.045	0.95ProGly: 0.95 ± 0.042	0.509ProHis: 0.509 ± 0.028	2.24ProIle: 2.24 ± 0.054	1.936ProLys: 1.936 ± 0.053	2.413ProLeu: 2.413 ± 0.048	0.634ProMet: 0.634 ± 0.03	1.64ProAsn: 1.64 ± 0.044	0.878ProPro: 0.878 ± 0.036	0.804ProGln: 0.804 ± 0.036	0.778ProArg: 0.778 ± 0.034	1.903ProSer: 1.903 ± 0.053	1.388ProThr: 1.388 ± 0.051	1.327ProVal: 1.327 ± 0.047	0.116ProTrp: 0.116 ± 0.01	1.132ProTyr: 1.132 ± 0.042	0.009ProXaa: 0.009 ± 0.003
Gln	0.607GlnAla: 0.607 ± 0.031	0.409GlnCys: 0.409 ± 0.024	1.169GlnAsp: 1.169 ± 0.038	1.804GlnGlu: 1.804 ± 0.052	1.006GlnPhe: 1.006 ± 0.032	0.713GlnGly: 0.713 ± 0.028	0.473GlnHis: 0.473 ± 0.026	2.438GlnIle: 2.438 ± 0.06	2.577GlnLys: 2.577 ± 0.067	2.157GlnLeu: 2.157 ± 0.055	0.631GlnMet: 0.631 ± 0.03	2.237GlnAsn: 2.237 ± 0.065	0.736GlnPro: 0.736 ± 0.04	0.877GlnGln: 0.877 ± 0.039	0.956GlnArg: 0.956 ± 0.034	1.544GlnSer: 1.544 ± 0.05	1.159GlnThr: 1.159 ± 0.037	0.879GlnVal: 0.879 ± 0.034	0.142GlnTrp: 0.142 ± 0.015	1.142GlnTyr: 1.142 ± 0.04	0.006GlnXaa: 0.006 ± 0.003
Arg	0.964ArgAla: 0.964 ± 0.038	0.563ArgCys: 0.563 ± 0.023	1.831ArgAsp: 1.831 ± 0.058	2.504ArgGlu: 2.504 ± 0.061	1.523ArgPhe: 1.523 ± 0.046	1.314ArgGly: 1.314 ± 0.044	0.499ArgHis: 0.499 ± 0.024	4.145ArgIle: 4.145 ± 0.07	3.952ArgLys: 3.952 ± 0.081	2.952ArgLeu: 2.952 ± 0.062	0.943ArgMet: 0.943 ± 0.035	3.272ArgAsn: 3.272 ± 0.076	0.717ArgPro: 0.717 ± 0.036	0.618ArgGln: 0.618 ± 0.027	1.843ArgArg: 1.843 ± 0.056	2.025ArgSer: 2.025 ± 0.044	1.467ArgThr: 1.467 ± 0.044	1.576ArgVal: 1.576 ± 0.042	0.185ArgTrp: 0.185 ± 0.015	1.677ArgTyr: 1.677 ± 0.047	0.002ArgXaa: 0.002 ± 0.002
Ser	1.876SerAla: 1.876 ± 0.055	1.012SerCys: 1.012 ± 0.039	3.811SerAsp: 3.811 ± 0.063	4.14SerGlu: 4.14 ± 0.094	3.73SerPhe: 3.73 ± 0.075	2.521SerGly: 2.521 ± 0.07	1.033SerHis: 1.033 ± 0.04	7.095SerIle: 7.095 ± 0.108	6.493SerLys: 6.493 ± 0.095	6.337SerLeu: 6.337 ± 0.107	1.399SerMet: 1.399 ± 0.037	5.558SerAsn: 5.558 ± 0.106	1.45SerPro: 1.45 ± 0.048	1.47SerGln: 1.47 ± 0.05	2.015SerArg: 2.015 ± 0.056	4.872SerSer: 4.872 ± 0.107	3.495SerThr: 3.495 ± 0.075	3.112SerVal: 3.112 ± 0.073	0.295SerTrp: 0.295 ± 0.018	2.994SerTyr: 2.994 ± 0.064	0.009SerXaa: 0.009 ± 0.004
Thr	1.482ThrAla: 1.482 ± 0.051	0.618ThrCys: 0.618 ± 0.026	2.765ThrAsp: 2.765 ± 0.068	3.696ThrGlu: 3.696 ± 0.073	2.494ThrPhe: 2.494 ± 0.059	1.878ThrGly: 1.878 ± 0.055	0.897ThrHis: 0.897 ± 0.03	4.997ThrIle: 4.997 ± 0.09	4.479ThrLys: 4.479 ± 0.076	4.593ThrLeu: 4.593 ± 0.076	0.928ThrMet: 0.928 ± 0.036	4.507ThrAsn: 4.507 ± 0.102	1.584ThrPro: 1.584 ± 0.057	1.269ThrGln: 1.269 ± 0.036	1.391ThrArg: 1.391 ± 0.042	3.087ThrSer: 3.087 ± 0.067	3.338ThrThr: 3.338 ± 0.117	2.308ThrVal: 2.308 ± 0.055	0.188ThrTrp: 0.188 ± 0.014	2.183ThrTyr: 2.183 ± 0.05	0.006ThrXaa: 0.006 ± 0.003
Val	1.462ValAla: 1.462 ± 0.047	0.842ValCys: 0.842 ± 0.037	2.945ValAsp: 2.945 ± 0.062	3.749ValGlu: 3.749 ± 0.083	2.485ValPhe: 2.485 ± 0.054	1.868ValGly: 1.868 ± 0.049	0.707ValHis: 0.707 ± 0.027	4.088ValIle: 4.088 ± 0.076	4.388ValLys: 4.388 ± 0.075	4.34ValLeu: 4.34 ± 0.089	0.96ValMet: 0.96 ± 0.039	3.119ValAsn: 3.119 ± 0.062	1.106ValPro: 1.106 ± 0.042	1.053ValGln: 1.053 ± 0.033	1.468ValArg: 1.468 ± 0.045	2.799ValSer: 2.799 ± 0.051	1.88ValThr: 1.88 ± 0.058	2.458ValVal: 2.458 ± 0.061	0.232ValTrp: 0.232 ± 0.018	2.211ValTyr: 2.211 ± 0.053	0.006ValXaa: 0.006 ± 0.003
Trp	0.15TrpAla: 0.15 ± 0.014	0.081TrpCys: 0.081 ± 0.01	0.277TrpAsp: 0.277 ± 0.018	0.282TrpGlu: 0.282 ± 0.02	0.21TrpPhe: 0.21 ± 0.016	0.186TrpGly: 0.186 ± 0.015	0.049TrpHis: 0.049 ± 0.008	0.469TrpIle: 0.469 ± 0.026	0.471TrpLys: 0.471 ± 0.022	0.373TrpLeu: 0.373 ± 0.022	0.165TrpMet: 0.165 ± 0.015	0.399TrpAsn: 0.399 ± 0.023	0.109TrpPro: 0.109 ± 0.012	0.085TrpGln: 0.085 ± 0.008	0.199TrpArg: 0.199 ± 0.016	0.308TrpSer: 0.308 ± 0.018	0.181TrpThr: 0.181 ± 0.015	0.199TrpVal: 0.199 ± 0.016	0.058TrpTrp: 0.058 ± 0.009	0.264TrpTyr: 0.264 ± 0.019	0.001TrpXaa: 0.001 ± 0.001
Tyr	1.275TyrAla: 1.275 ± 0.039	0.8TyrCys: 0.8 ± 0.031	2.834TyrAsp: 2.834 ± 0.063	2.979TyrGlu: 2.979 ± 0.062	3.527TyrPhe: 3.527 ± 0.075	1.824TyrGly: 1.824 ± 0.051	0.845TyrHis: 0.845 ± 0.033	5.163TyrIle: 5.163 ± 0.092	5.61TyrLys: 5.61 ± 0.092	4.915TyrLeu: 4.915 ± 0.094	0.944TyrMet: 0.944 ± 0.031	5.254TyrAsn: 5.254 ± 0.106	1.167TyrPro: 1.167 ± 0.042	1.109TyrGln: 1.109 ± 0.039	1.512TyrArg: 1.512 ± 0.038	3.426TyrSer: 3.426 ± 0.063	2.622TyrThr: 2.622 ± 0.053	2.045TyrVal: 2.045 ± 0.048	0.236TyrTrp: 0.236 ± 0.016	2.722TyrTyr: 2.722 ± 0.072	0.013TyrXaa: 0.013 ± 0.004
Xaa	0.004XaaAla: 0.004 ± 0.002	0.002XaaCys: 0.002 ± 0.001	0.004XaaAsp: 0.004 ± 0.002	0.018XaaGlu: 0.018 ± 0.005	0.013XaaPhe: 0.013 ± 0.004	0.001XaaGly: 0.001 ± 0.001	0.004XaaHis: 0.004 ± 0.002	0.02XaaIle: 0.02 ± 0.005	0.017XaaLys: 0.017 ± 0.005	0.014XaaLeu: 0.014 ± 0.004	0.011XaaMet: 0.011 ± 0.004	0.014XaaAsn: 0.014 ± 0.004	0.006XaaPro: 0.006 ± 0.003	0.002XaaGln: 0.002 ± 0.002	0.007XaaArg: 0.007 ± 0.003	0.006XaaSer: 0.006 ± 0.003	0.006XaaThr: 0.006 ± 0.003	0.011XaaVal: 0.011 ± 0.004	0.0XaaTrp: 0.0 ± 0.0	0.006XaaTyr: 0.006 ± 0.003	0.004XaaXaa: 0.004 ± 0.002

Statistics based on 2499 proteins (844153 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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