Chryseobacterium sp. RU37D
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2783 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1N6RZ44|A0A1N6RZ44_9FLAO Opacity protein OS=Chryseobacterium sp. RU37D OX=1907397 GN=SAMN05880574_110103 PE=4 SV=1
MM1 pKa = 7.7 SDD3 pKa = 3.06 INIKK7 pKa = 8.65 ITDD10 pKa = 3.8 RR11 pKa = 11.84 DD12 pKa = 4.4 GISHH16 pKa = 7.21 DD17 pKa = 3.93 VVAPTDD23 pKa = 3.29 MSMNLMEE30 pKa = 5.76 IIRR33 pKa = 11.84 SYY35 pKa = 11.15 EE36 pKa = 3.71 LAEE39 pKa = 4.26 EE40 pKa = 4.58 GTIGVCGGMAMCASCQVYY58 pKa = 10.1 VINDD62 pKa = 3.73 PGLDD66 pKa = 3.36 EE67 pKa = 4.72 MGAEE71 pKa = 4.21 EE72 pKa = 5.26 DD73 pKa = 3.93 AMLGEE78 pKa = 4.57 AFHH81 pKa = 6.82 VKK83 pKa = 10.34 DD84 pKa = 4.16 NSRR87 pKa = 11.84 LGCQLHH93 pKa = 6.4 IVPEE97 pKa = 4.2 MEE99 pKa = 3.93 GLEE102 pKa = 4.18 VEE104 pKa = 4.35 IAPYY108 pKa = 9.53 PP109 pKa = 3.68
Molecular weight: 11.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.801
IPC2_protein 4.024
IPC_protein 3.948
Toseland 3.77
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.821
Rodwell 3.795
Grimsley 3.694
Solomon 3.91
Lehninger 3.859
Nozaki 4.037
DTASelect 4.19
Thurlkill 3.808
EMBOSS 3.834
Sillero 4.075
Patrickios 0.947
IPC_peptide 3.91
IPC2_peptide 4.05
IPC2.peptide.svr19 3.951
Protein with the highest isoelectric point:
>tr|A0A1N6REH5|A0A1N6REH5_9FLAO Curli production assembly/transport component CsgG OS=Chryseobacterium sp. RU37D OX=1907397 GN=SAMN05880574_10938 PE=4 SV=1
MM1 pKa = 7.84 PSGKK5 pKa = 9.32 KK6 pKa = 9.59 RR7 pKa = 11.84 KK8 pKa = 7.05 RR9 pKa = 11.84 HH10 pKa = 5.11 KK11 pKa = 10.59 VATHH15 pKa = 5.21 KK16 pKa = 10.3 RR17 pKa = 11.84 KK18 pKa = 9.62 KK19 pKa = 9.04 RR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 ANRR25 pKa = 11.84 HH26 pKa = 4.5 KK27 pKa = 10.78 KK28 pKa = 9.37 KK29 pKa = 10.83
Molecular weight: 3.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.474
IPC2_protein 11.082
IPC_protein 12.618
Toseland 12.808
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.261
Rodwell 12.705
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.793
DTASelect 12.793
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.427
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.035
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2783
0
2783
881348
25
2360
316.7
35.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.145 ± 0.054
0.736 ± 0.014
5.327 ± 0.032
6.554 ± 0.053
5.475 ± 0.044
6.454 ± 0.048
1.678 ± 0.02
8.135 ± 0.044
8.447 ± 0.053
8.867 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.387 ± 0.022
6.542 ± 0.042
3.449 ± 0.026
3.434 ± 0.027
3.229 ± 0.029
6.439 ± 0.035
5.519 ± 0.051
5.974 ± 0.035
1.036 ± 0.018
4.17 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here