Amino acid dipepetide frequency for Chryseobacterium sp. RU37D

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.236AlaAla: 4.236 ± 0.096
0.496AlaCys: 0.496 ± 0.027
3.253AlaAsp: 3.253 ± 0.068
4.205AlaGlu: 4.205 ± 0.077
3.141AlaPhe: 3.141 ± 0.067
4.39AlaGly: 4.39 ± 0.089
1.019AlaHis: 1.019 ± 0.036
4.66AlaIle: 4.66 ± 0.081
5.0AlaLys: 5.0 ± 0.087
5.593AlaLeu: 5.593 ± 0.083
1.522AlaMet: 1.522 ± 0.041
3.44AlaAsn: 3.44 ± 0.085
1.877AlaPro: 1.877 ± 0.049
2.41AlaGln: 2.41 ± 0.057
1.86AlaArg: 1.86 ± 0.052
3.832AlaSer: 3.832 ± 0.066
3.4AlaThr: 3.4 ± 0.086
4.202AlaVal: 4.202 ± 0.076
0.568AlaTrp: 0.568 ± 0.029
2.352AlaTyr: 2.352 ± 0.062
0.0AlaXaa: 0.0 ± 0.0
Cys
0.425CysAla: 0.425 ± 0.021
0.082CysCys: 0.082 ± 0.01
0.389CysAsp: 0.389 ± 0.022
0.45CysGlu: 0.45 ± 0.025
0.425CysPhe: 0.425 ± 0.023
0.642CysGly: 0.642 ± 0.03
0.175CysHis: 0.175 ± 0.015
0.627CysIle: 0.627 ± 0.026
0.477CysLys: 0.477 ± 0.026
0.615CysLeu: 0.615 ± 0.029
0.163CysMet: 0.163 ± 0.012
0.415CysAsn: 0.415 ± 0.025
0.331CysPro: 0.331 ± 0.023
0.203CysGln: 0.203 ± 0.016
0.221CysArg: 0.221 ± 0.016
0.54CysSer: 0.54 ± 0.024
0.419CysThr: 0.419 ± 0.023
0.411CysVal: 0.411 ± 0.024
0.059CysTrp: 0.059 ± 0.008
0.293CysTyr: 0.293 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
3.245AspAla: 3.245 ± 0.065
0.366AspCys: 0.366 ± 0.02
2.545AspAsp: 2.545 ± 0.062
3.978AspGlu: 3.978 ± 0.08
3.868AspPhe: 3.868 ± 0.07
3.369AspGly: 3.369 ± 0.07
0.885AspHis: 0.885 ± 0.034
4.385AspIle: 4.385 ± 0.085
4.519AspLys: 4.519 ± 0.07
4.997AspLeu: 4.997 ± 0.075
1.112AspMet: 1.112 ± 0.036
2.871AspAsn: 2.871 ± 0.056
1.856AspPro: 1.856 ± 0.05
1.688AspGln: 1.688 ± 0.051
1.744AspArg: 1.744 ± 0.046
2.857AspSer: 2.857 ± 0.063
2.253AspThr: 2.253 ± 0.055
3.228AspVal: 3.228 ± 0.068
0.723AspTrp: 0.723 ± 0.03
2.779AspTyr: 2.779 ± 0.065
0.0AspXaa: 0.0 ± 0.0
Glu
3.831GluAla: 3.831 ± 0.086
0.348GluCys: 0.348 ± 0.022
3.351GluAsp: 3.351 ± 0.068
4.908GluGlu: 4.908 ± 0.114
3.293GluPhe: 3.293 ± 0.071
3.389GluGly: 3.389 ± 0.072
1.001GluHis: 1.001 ± 0.033
6.017GluIle: 6.017 ± 0.098
7.048GluLys: 7.048 ± 0.095
5.521GluLeu: 5.521 ± 0.095
1.686GluMet: 1.686 ± 0.046
5.421GluAsn: 5.421 ± 0.088
1.402GluPro: 1.402 ± 0.045
2.078GluGln: 2.078 ± 0.057
2.4GluArg: 2.4 ± 0.062
3.321GluSer: 3.321 ± 0.068
3.315GluThr: 3.315 ± 0.073
4.028GluVal: 4.028 ± 0.067
0.674GluTrp: 0.674 ± 0.028
2.512GluTyr: 2.512 ± 0.058
0.0GluXaa: 0.0 ± 0.0
Phe
3.275PheAla: 3.275 ± 0.067
0.483PheCys: 0.483 ± 0.024
3.196PheAsp: 3.196 ± 0.063
3.269PheGlu: 3.269 ± 0.066
3.029PhePhe: 3.029 ± 0.076
3.913PheGly: 3.913 ± 0.072
0.967PheHis: 0.967 ± 0.035
4.233PheIle: 4.233 ± 0.094
3.875PheLys: 3.875 ± 0.072
5.091PheLeu: 5.091 ± 0.098
1.231PheMet: 1.231 ± 0.036
3.326PheAsn: 3.326 ± 0.068
1.932PhePro: 1.932 ± 0.05
1.604PheGln: 1.604 ± 0.047
1.796PheArg: 1.796 ± 0.047
4.247PheSer: 4.247 ± 0.076
3.11PheThr: 3.11 ± 0.064
3.155PheVal: 3.155 ± 0.064
0.672PheTrp: 0.672 ± 0.026
2.341PheTyr: 2.341 ± 0.059
0.0PheXaa: 0.0 ± 0.0
Gly
4.093GlyAla: 4.093 ± 0.074
0.564GlyCys: 0.564 ± 0.029
2.989GlyAsp: 2.989 ± 0.068
3.553GlyGlu: 3.553 ± 0.069
3.707GlyPhe: 3.707 ± 0.078
4.708GlyGly: 4.708 ± 0.111
1.029GlyHis: 1.029 ± 0.041
5.621GlyIle: 5.621 ± 0.102
5.608GlyLys: 5.608 ± 0.082
5.455GlyLeu: 5.455 ± 0.088
1.671GlyMet: 1.671 ± 0.047
4.236GlyAsn: 4.236 ± 0.084
1.264GlyPro: 1.264 ± 0.041
1.966GlyGln: 1.966 ± 0.051
2.132GlyArg: 2.132 ± 0.049
4.11GlySer: 4.11 ± 0.083
4.15GlyThr: 4.15 ± 0.096
4.062GlyVal: 4.062 ± 0.079
0.777GlyTrp: 0.777 ± 0.034
2.847GlyTyr: 2.847 ± 0.059
0.0GlyXaa: 0.0 ± 0.0
His
0.902HisAla: 0.902 ± 0.034
0.168HisCys: 0.168 ± 0.015
0.843HisAsp: 0.843 ± 0.033
1.004HisGlu: 1.004 ± 0.038
1.129HisPhe: 1.129 ± 0.033
1.05HisGly: 1.05 ± 0.036
0.465HisHis: 0.465 ± 0.027
1.348HisIle: 1.348 ± 0.041
1.143HisLys: 1.143 ± 0.041
1.612HisLeu: 1.612 ± 0.045
0.33HisMet: 0.33 ± 0.018
0.942HisAsn: 0.942 ± 0.034
0.867HisPro: 0.867 ± 0.033
0.667HisGln: 0.667 ± 0.029
0.607HisArg: 0.607 ± 0.022
1.089HisSer: 1.089 ± 0.04
0.815HisThr: 0.815 ± 0.036
0.767HisVal: 0.767 ± 0.031
0.222HisTrp: 0.222 ± 0.017
0.809HisTyr: 0.809 ± 0.031
0.0HisXaa: 0.0 ± 0.0
Ile
5.217IleAla: 5.217 ± 0.081
0.736IleCys: 0.736 ± 0.03
4.675IleAsp: 4.675 ± 0.071
5.126IleGlu: 5.126 ± 0.083
4.5IlePhe: 4.5 ± 0.087
5.084IleGly: 5.084 ± 0.093
1.397IleHis: 1.397 ± 0.037
6.532IleIle: 6.532 ± 0.118
6.227IleLys: 6.227 ± 0.09
7.601IleLeu: 7.601 ± 0.116
1.559IleMet: 1.559 ± 0.041
5.181IleAsn: 5.181 ± 0.085
3.41IlePro: 3.41 ± 0.067
2.714IleGln: 2.714 ± 0.058
2.447IleArg: 2.447 ± 0.052
6.101IleSer: 6.101 ± 0.087
4.651IleThr: 4.651 ± 0.084
4.489IleVal: 4.489 ± 0.08
0.655IleTrp: 0.655 ± 0.026
3.054IleTyr: 3.054 ± 0.064
0.0IleXaa: 0.0 ± 0.0
Lys
4.955LysAla: 4.955 ± 0.077
0.371LysCys: 0.371 ± 0.023
5.1LysAsp: 5.1 ± 0.088
6.282LysGlu: 6.282 ± 0.108
3.686LysPhe: 3.686 ± 0.075
4.626LysGly: 4.626 ± 0.078
1.278LysHis: 1.278 ± 0.042
7.73LysIle: 7.73 ± 0.108
7.854LysLys: 7.854 ± 0.124
6.657LysLeu: 6.657 ± 0.109
2.451LysMet: 2.451 ± 0.048
6.754LysAsn: 6.754 ± 0.101
2.542LysPro: 2.542 ± 0.055
2.708LysGln: 2.708 ± 0.055
2.626LysArg: 2.626 ± 0.055
4.761LysSer: 4.761 ± 0.083
4.835LysThr: 4.835 ± 0.07
5.009LysVal: 5.009 ± 0.077
0.761LysTrp: 0.761 ± 0.033
3.488LysTyr: 3.488 ± 0.064
0.0LysXaa: 0.0 ± 0.0
Leu
5.431LeuAla: 5.431 ± 0.085
0.677LeuCys: 0.677 ± 0.031
4.629LeuAsp: 4.629 ± 0.082
5.733LeuGlu: 5.733 ± 0.106
4.681LeuPhe: 4.681 ± 0.084
5.802LeuGly: 5.802 ± 0.082
1.582LeuHis: 1.582 ± 0.041
6.829LeuIle: 6.829 ± 0.112
8.0LeuLys: 8.0 ± 0.116
7.855LeuLeu: 7.855 ± 0.112
2.277LeuMet: 2.277 ± 0.056
5.686LeuAsn: 5.686 ± 0.087
3.48LeuPro: 3.48 ± 0.064
3.202LeuGln: 3.202 ± 0.067
2.942LeuArg: 2.942 ± 0.057
6.356LeuSer: 6.356 ± 0.089
4.753LeuThr: 4.753 ± 0.082
4.838LeuVal: 4.838 ± 0.077
0.777LeuTrp: 0.777 ± 0.03
3.141LeuTyr: 3.141 ± 0.061
0.0LeuXaa: 0.0 ± 0.0
Met
1.57MetAla: 1.57 ± 0.043
0.146MetCys: 0.146 ± 0.011
1.18MetAsp: 1.18 ± 0.037
1.59MetGlu: 1.59 ± 0.046
0.927MetPhe: 0.927 ± 0.039
1.55MetGly: 1.55 ± 0.051
0.346MetHis: 0.346 ± 0.019
1.84MetIle: 1.84 ± 0.047
2.739MetLys: 2.739 ± 0.056
2.04MetLeu: 2.04 ± 0.046
0.761MetMet: 0.761 ± 0.034
1.645MetAsn: 1.645 ± 0.04
0.829MetPro: 0.829 ± 0.028
0.819MetGln: 0.819 ± 0.029
0.843MetArg: 0.843 ± 0.032
1.463MetSer: 1.463 ± 0.04
1.247MetThr: 1.247 ± 0.034
1.425MetVal: 1.425 ± 0.043
0.182MetTrp: 0.182 ± 0.014
0.724MetTyr: 0.724 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
4.09AsnAla: 4.09 ± 0.082
0.444AsnCys: 0.444 ± 0.024
3.211AsnAsp: 3.211 ± 0.069
3.867AsnGlu: 3.867 ± 0.073
3.752AsnPhe: 3.752 ± 0.083
4.295AsnGly: 4.295 ± 0.082
1.095AsnHis: 1.095 ± 0.038
5.63AsnIle: 5.63 ± 0.073
4.533AsnLys: 4.533 ± 0.078
5.824AsnLeu: 5.824 ± 0.102
1.385AsnMet: 1.385 ± 0.04
4.386AsnAsn: 4.386 ± 0.085
3.126AsnPro: 3.126 ± 0.061
2.402AsnGln: 2.402 ± 0.059
1.984AsnArg: 1.984 ± 0.05
4.174AsnSer: 4.174 ± 0.074
3.501AsnThr: 3.501 ± 0.08
3.774AsnVal: 3.774 ± 0.085
0.768AsnTrp: 0.768 ± 0.032
3.177AsnTyr: 3.177 ± 0.075
0.0AsnXaa: 0.0 ± 0.0
Pro
2.152ProAla: 2.152 ± 0.059
0.227ProCys: 0.227 ± 0.016
1.96ProAsp: 1.96 ± 0.047
2.944ProGlu: 2.944 ± 0.066
1.871ProPhe: 1.871 ± 0.045
1.988ProGly: 1.988 ± 0.059
0.567ProHis: 0.567 ± 0.023
2.473ProIle: 2.473 ± 0.057
2.833ProLys: 2.833 ± 0.068
2.713ProLeu: 2.713 ± 0.058
0.803ProMet: 0.803 ± 0.029
2.332ProAsn: 2.332 ± 0.057
0.801ProPro: 0.801 ± 0.039
1.279ProGln: 1.279 ± 0.042
0.938ProArg: 0.938 ± 0.036
2.18ProSer: 2.18 ± 0.053
2.091ProThr: 2.091 ± 0.062
2.576ProVal: 2.576 ± 0.065
0.304ProTrp: 0.304 ± 0.021
1.461ProTyr: 1.461 ± 0.042
0.0ProXaa: 0.0 ± 0.0
Gln
1.94GlnAla: 1.94 ± 0.052
0.159GlnCys: 0.159 ± 0.014
1.666GlnAsp: 1.666 ± 0.044
2.258GlnGlu: 2.258 ± 0.048
1.702GlnPhe: 1.702 ± 0.042
1.804GlnGly: 1.804 ± 0.051
0.537GlnHis: 0.537 ± 0.025
2.846GlnIle: 2.846 ± 0.062
3.473GlnLys: 3.473 ± 0.08
3.135GlnLeu: 3.135 ± 0.067
0.863GlnMet: 0.863 ± 0.03
2.637GlnAsn: 2.637 ± 0.071
1.076GlnPro: 1.076 ± 0.042
1.408GlnGln: 1.408 ± 0.054
1.249GlnArg: 1.249 ± 0.038
1.987GlnSer: 1.987 ± 0.042
1.906GlnThr: 1.906 ± 0.045
1.77GlnVal: 1.77 ± 0.05
0.364GlnTrp: 0.364 ± 0.023
1.561GlnTyr: 1.561 ± 0.041
0.0GlnXaa: 0.0 ± 0.0
Arg
1.811ArgAla: 1.811 ± 0.046
0.186ArgCys: 0.186 ± 0.017
1.68ArgAsp: 1.68 ± 0.041
2.15ArgGlu: 2.15 ± 0.055
1.832ArgPhe: 1.832 ± 0.052
1.777ArgGly: 1.777 ± 0.047
0.566ArgHis: 0.566 ± 0.026
2.756ArgIle: 2.756 ± 0.051
3.088ArgLys: 3.088 ± 0.061
2.904ArgLeu: 2.904 ± 0.061
0.924ArgMet: 0.924 ± 0.03
2.222ArgAsn: 2.222 ± 0.052
1.076ArgPro: 1.076 ± 0.038
1.084ArgGln: 1.084 ± 0.035
1.287ArgArg: 1.287 ± 0.043
1.743ArgSer: 1.743 ± 0.051
1.658ArgThr: 1.658 ± 0.042
1.957ArgVal: 1.957 ± 0.048
0.322ArgTrp: 0.322 ± 0.018
1.267ArgTyr: 1.267 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
3.9SerAla: 3.9 ± 0.074
0.593SerCys: 0.593 ± 0.026
3.348SerAsp: 3.348 ± 0.063
4.059SerGlu: 4.059 ± 0.081
3.749SerPhe: 3.749 ± 0.068
4.862SerGly: 4.862 ± 0.082
1.037SerHis: 1.037 ± 0.033
4.987SerIle: 4.987 ± 0.091
4.948SerLys: 4.948 ± 0.079
5.932SerLeu: 5.932 ± 0.095
1.453SerMet: 1.453 ± 0.045
3.621SerAsn: 3.621 ± 0.072
2.227SerPro: 2.227 ± 0.049
2.387SerGln: 2.387 ± 0.057
1.924SerArg: 1.924 ± 0.045
4.313SerSer: 4.313 ± 0.093
3.542SerThr: 3.542 ± 0.093
4.056SerVal: 4.056 ± 0.076
0.762SerTrp: 0.762 ± 0.029
2.688SerTyr: 2.688 ± 0.064
0.0SerXaa: 0.0 ± 0.0
Thr
3.702ThrAla: 3.702 ± 0.068
0.342ThrCys: 0.342 ± 0.021
3.124ThrAsp: 3.124 ± 0.066
3.36ThrGlu: 3.36 ± 0.063
2.857ThrPhe: 2.857 ± 0.062
4.166ThrGly: 4.166 ± 0.096
0.882ThrHis: 0.882 ± 0.027
4.274ThrIle: 4.274 ± 0.07
4.031ThrLys: 4.031 ± 0.074
4.895ThrLeu: 4.895 ± 0.082
1.056ThrMet: 1.056 ± 0.036
3.201ThrAsn: 3.201 ± 0.076
2.483ThrPro: 2.483 ± 0.061
1.859ThrGln: 1.859 ± 0.054
1.523ThrArg: 1.523 ± 0.043
3.618ThrSer: 3.618 ± 0.074
3.484ThrThr: 3.484 ± 0.114
3.549ThrVal: 3.549 ± 0.076
0.54ThrTrp: 0.54 ± 0.027
2.25ThrTyr: 2.25 ± 0.055
0.0ThrXaa: 0.0 ± 0.0
Val
3.77ValAla: 3.77 ± 0.078
0.514ValCys: 0.514 ± 0.027
3.193ValAsp: 3.193 ± 0.07
3.737ValGlu: 3.737 ± 0.068
3.321ValPhe: 3.321 ± 0.067
3.824ValGly: 3.824 ± 0.077
0.92ValHis: 0.92 ± 0.031
4.663ValIle: 4.663 ± 0.087
4.987ValLys: 4.987 ± 0.089
5.419ValLeu: 5.419 ± 0.07
1.494ValMet: 1.494 ± 0.05
3.644ValAsn: 3.644 ± 0.063
2.196ValPro: 2.196 ± 0.052
1.845ValGln: 1.845 ± 0.049
1.869ValArg: 1.869 ± 0.052
4.285ValSer: 4.285 ± 0.069
3.22ValThr: 3.22 ± 0.084
3.781ValVal: 3.781 ± 0.083
0.596ValTrp: 0.596 ± 0.026
2.467ValTyr: 2.467 ± 0.057
0.0ValXaa: 0.0 ± 0.0
Trp
0.627TrpAla: 0.627 ± 0.027
0.093TrpCys: 0.093 ± 0.011
0.574TrpAsp: 0.574 ± 0.029
0.658TrpGlu: 0.658 ± 0.031
0.553TrpPhe: 0.553 ± 0.029
0.723TrpGly: 0.723 ± 0.035
0.182TrpHis: 0.182 ± 0.017
0.801TrpIle: 0.801 ± 0.03
0.979TrpLys: 0.979 ± 0.035
0.902TrpLeu: 0.902 ± 0.03
0.325TrpMet: 0.325 ± 0.021
0.777TrpAsn: 0.777 ± 0.031
0.168TrpPro: 0.168 ± 0.013
0.376TrpGln: 0.376 ± 0.024
0.366TrpArg: 0.366 ± 0.02
0.582TrpSer: 0.582 ± 0.028
0.536TrpThr: 0.536 ± 0.026
0.605TrpVal: 0.605 ± 0.029
0.132TrpTrp: 0.132 ± 0.013
0.404TrpTyr: 0.404 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.281TyrAla: 2.281 ± 0.056
0.368TyrCys: 0.368 ± 0.023
2.363TyrAsp: 2.363 ± 0.06
2.412TyrGlu: 2.412 ± 0.06
2.722TyrPhe: 2.722 ± 0.062
2.58TyrGly: 2.58 ± 0.053
0.851TyrHis: 0.851 ± 0.034
3.101TyrIle: 3.101 ± 0.06
3.111TyrLys: 3.111 ± 0.065
3.914TyrLeu: 3.914 ± 0.07
0.798TyrMet: 0.798 ± 0.028
2.683TyrAsn: 2.683 ± 0.058
1.552TyrPro: 1.552 ± 0.041
1.642TyrGln: 1.642 ± 0.048
1.531TyrArg: 1.531 ± 0.048
2.831TyrSer: 2.831 ± 0.059
2.308TyrThr: 2.308 ± 0.057
2.064TyrVal: 2.064 ± 0.05
0.503TyrTrp: 0.503 ± 0.029
2.088TyrTyr: 2.088 ± 0.058
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2783 proteins (881348 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski