Canine papillomavirus 22
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346M198|A0A346M198_9PAPI Protein E6 OS=Canine papillomavirus 22 OX=2304620 GN=E6 PE=3 SV=1
MM1 pKa = 7.0 MACIMRR7 pKa = 11.84 TRR9 pKa = 11.84 MEE11 pKa = 4.06 NRR13 pKa = 11.84 CIFAILKK20 pKa = 6.02 QTPVALAPEE29 pKa = 4.91 AYY31 pKa = 10.22 GEE33 pKa = 4.08 FCIRR37 pKa = 11.84 MSACLPLFLARR48 pKa = 11.84 DD49 pKa = 3.79 PVPRR53 pKa = 11.84 PPAAPTPGPGGDD65 pKa = 3.3 RR66 pKa = 11.84 IGPRR70 pKa = 11.84 PHH72 pKa = 6.82 NPQDD76 pKa = 3.82 PNQAPPPQPDD86 pKa = 3.18 TRR88 pKa = 11.84 GDD90 pKa = 3.55 RR91 pKa = 11.84 QSPGGGEE98 pKa = 3.76 QGPGDD103 pKa = 4.35 EE104 pKa = 4.59 EE105 pKa = 6.37 DD106 pKa = 3.64 EE107 pKa = 6.04 DD108 pKa = 4.23 EE109 pKa = 6.57 DD110 pKa = 4.3 EE111 pKa = 6.27 DD112 pKa = 4.58 EE113 pKa = 5.44 DD114 pKa = 5.11 RR115 pKa = 11.84 PPPPPPPPAACNCQGRR131 pKa = 11.84 TPQRR135 pKa = 11.84 EE136 pKa = 3.47 RR137 pKa = 11.84 DD138 pKa = 3.06 RR139 pKa = 11.84 RR140 pKa = 11.84 RR141 pKa = 11.84 RR142 pKa = 11.84 RR143 pKa = 11.84 RR144 pKa = 11.84 GRR146 pKa = 11.84 ARR148 pKa = 11.84 LRR150 pKa = 11.84 FQGPEE155 pKa = 3.64 GGEE158 pKa = 3.96 EE159 pKa = 3.92 EE160 pKa = 4.22 KK161 pKa = 10.98 AHH163 pKa = 6.07 YY164 pKa = 10.08 EE165 pKa = 3.89 EE166 pKa = 6.43 AGEE169 pKa = 4.19 KK170 pKa = 10.79 DD171 pKa = 3.55 PDD173 pKa = 3.96 EE174 pKa = 4.97 EE175 pKa = 5.84 DD176 pKa = 3.03 PHH178 pKa = 7.26 QPPTEE183 pKa = 4.07 EE184 pKa = 4.28 EE185 pKa = 4.48 KK186 pKa = 11.14 EE187 pKa = 3.83 NSPPHH192 pKa = 6.39 KK193 pKa = 9.38 PAPLRR198 pKa = 11.84 RR199 pKa = 11.84 PYY201 pKa = 10.55 YY202 pKa = 10.15 SPPPGPLGDD211 pKa = 3.77 STEE214 pKa = 4.97 DD215 pKa = 3.79 DD216 pKa = 4.21 DD217 pKa = 5.67 EE218 pKa = 6.8 GPASLLKK225 pKa = 10.85 DD226 pKa = 4.23 LGRR229 pKa = 11.84 KK230 pKa = 8.27 WGADD234 pKa = 3.39 LDD236 pKa = 4.22 ALHH239 pKa = 6.54 HH240 pKa = 6.4 KK241 pKa = 9.85 IWGDD245 pKa = 3.51 LEE247 pKa = 4.08 KK248 pKa = 11.14 LRR250 pKa = 11.84 DD251 pKa = 3.74 RR252 pKa = 11.84 LGASQQ257 pKa = 3.78
Molecular weight: 28.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.974
IPC2_protein 4.952
IPC_protein 4.889
Toseland 4.774
ProMoST 5.003
Dawson 4.851
Bjellqvist 4.991
Wikipedia 4.711
Rodwell 4.749
Grimsley 4.685
Solomon 4.851
Lehninger 4.8
Nozaki 4.965
DTASelect 5.118
Thurlkill 4.774
EMBOSS 4.736
Sillero 5.029
Patrickios 4.266
IPC_peptide 4.851
IPC2_peptide 5.029
IPC2.peptide.svr19 4.998
Protein with the highest isoelectric point:
>tr|A0A346M197|A0A346M197_9PAPI E4 OS=Canine papillomavirus 22 OX=2304620 PE=4 SV=1
MM1 pKa = 6.81 QNLRR5 pKa = 11.84 IRR7 pKa = 11.84 YY8 pKa = 8.63 DD9 pKa = 3.41 AVQEE13 pKa = 3.99 ALFAHH18 pKa = 6.39 YY19 pKa = 9.71 EE20 pKa = 4.04 QGSKK24 pKa = 10.54 SLNDD28 pKa = 3.23 QVSYY32 pKa = 10.32 WEE34 pKa = 3.79 LRR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 EE39 pKa = 4.01 SAILYY44 pKa = 6.54 WARR47 pKa = 11.84 KK48 pKa = 9.28 NGISTLGFQPVPSLQSSEE66 pKa = 5.1 ANAKK70 pKa = 9.68 SAIGMSLTLQSLLASPFGQEE90 pKa = 3.56 PWTLPEE96 pKa = 4.29 TSLEE100 pKa = 4.1 MWNTPPKK107 pKa = 10.91 NCMKK111 pKa = 10.55 KK112 pKa = 9.64 HH113 pKa = 5.05 GFTVEE118 pKa = 3.66 VHH120 pKa = 6.56 FDD122 pKa = 3.65 NDD124 pKa = 3.66 PEE126 pKa = 4.22 NAFPYY131 pKa = 9.6 TCWQSIYY138 pKa = 10.67 YY139 pKa = 9.74 QDD141 pKa = 4.39 AEE143 pKa = 4.23 TNEE146 pKa = 4.15 WVKK149 pKa = 10.37 TSGKK153 pKa = 9.1 VAHH156 pKa = 7.18 DD157 pKa = 3.21 GMYY160 pKa = 10.87 YY161 pKa = 10.05 EE162 pKa = 5.78 DD163 pKa = 4.93 KK164 pKa = 11.01 DD165 pKa = 3.8 GEE167 pKa = 4.41 QVYY170 pKa = 9.47 FCHH173 pKa = 7.11 FEE175 pKa = 3.86 TDD177 pKa = 2.82 ASRR180 pKa = 11.84 FSTRR184 pKa = 11.84 GIWRR188 pKa = 11.84 VLYY191 pKa = 10.96 KK192 pKa = 10.37 NVCLSASVSSSGSGAPAAGGSNTGPWWGQDD222 pKa = 2.82 WPEE225 pKa = 4.13 APQPAGPQPSAASSTRR241 pKa = 11.84 HH242 pKa = 5.25 SRR244 pKa = 11.84 GSPEE248 pKa = 3.53 PRR250 pKa = 11.84 RR251 pKa = 11.84 RR252 pKa = 11.84 GTGSRR257 pKa = 11.84 GRR259 pKa = 11.84 GGRR262 pKa = 11.84 GRR264 pKa = 11.84 GRR266 pKa = 11.84 GRR268 pKa = 11.84 GSSASPSAPACRR280 pKa = 11.84 LQLPGPDD287 pKa = 3.48 TPEE290 pKa = 5.62 GEE292 pKa = 4.2 GSEE295 pKa = 4.21 EE296 pKa = 4.16 EE297 pKa = 4.28 EE298 pKa = 4.3 EE299 pKa = 5.18 GPCEE303 pKa = 4.15 AEE305 pKa = 4.33 VPRR308 pKa = 11.84 SRR310 pKa = 11.84 GRR312 pKa = 11.84 GGGKK316 pKa = 9.94 GPLRR320 pKa = 11.84 GGRR323 pKa = 11.84 GKK325 pKa = 10.4 RR326 pKa = 11.84 PRR328 pKa = 11.84 RR329 pKa = 11.84 GGPSPTTYY337 pKa = 10.05 RR338 pKa = 11.84 GRR340 pKa = 11.84 EE341 pKa = 4.34 GEE343 pKa = 4.0 QSTSQTCTPPAPILQPSTGSTGGLYY368 pKa = 10.04 RR369 pKa = 11.84 GRR371 pKa = 11.84 RR372 pKa = 11.84 RR373 pKa = 11.84 GPCLSPEE380 pKa = 4.34 GSWSKK385 pKa = 10.66 VGCRR389 pKa = 11.84 PRR391 pKa = 11.84 RR392 pKa = 11.84 SSSQDD397 pKa = 2.96 LGGPGEE403 pKa = 4.51 ASGQTGRR410 pKa = 11.84 VPIVVVKK417 pKa = 9.29 GEE419 pKa = 4.21 CNALKK424 pKa = 10.29 CYY426 pKa = 10.24 RR427 pKa = 11.84 LRR429 pKa = 11.84 LRR431 pKa = 11.84 RR432 pKa = 11.84 RR433 pKa = 11.84 HH434 pKa = 5.26 RR435 pKa = 11.84 HH436 pKa = 4.03 TFDD439 pKa = 4.91 FISTGFGWIGGEE451 pKa = 3.86 GRR453 pKa = 11.84 KK454 pKa = 9.59 VGRR457 pKa = 11.84 QRR459 pKa = 11.84 MLIAFSGYY467 pKa = 7.48 DD468 pKa = 3.09 QRR470 pKa = 11.84 DD471 pKa = 3.44 FFLGHH476 pKa = 5.32 VKK478 pKa = 9.88 IPKK481 pKa = 10.32 LMDD484 pKa = 3.23 VSLGEE489 pKa = 4.25 LDD491 pKa = 3.81 SLL493 pKa = 4.86
Molecular weight: 54.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.901
IPC2_protein 8.77
IPC_protein 8.77
Toseland 9.209
ProMoST 9.18
Dawson 9.575
Bjellqvist 9.472
Wikipedia 9.78
Rodwell 9.692
Grimsley 9.633
Solomon 9.633
Lehninger 9.575
Nozaki 9.589
DTASelect 9.37
Thurlkill 9.443
EMBOSS 9.706
Sillero 9.619
Patrickios 4.863
IPC_peptide 9.619
IPC2_peptide 8.507
IPC2.peptide.svr19 7.761
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2616
96
605
373.7
41.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.422 ± 0.567
2.599 ± 0.81
6.537 ± 0.788
6.307 ± 0.73
4.243 ± 0.764
8.142 ± 1.311
2.561 ± 0.38
3.593 ± 0.63
4.778 ± 0.86
8.142 ± 1.077
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.606 ± 0.433
3.211 ± 0.742
7.913 ± 1.438
4.434 ± 0.442
6.804 ± 1.075
7.645 ± 1.016
5.696 ± 0.453
5.657 ± 0.919
1.376 ± 0.311
2.332 ± 0.411
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here