Terrilactibacillus tamarindi
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2956 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6N8CQ77|A0A6N8CQ77_9BACI PDZ domain-containing protein OS=Terrilactibacillus tamarindi OX=2599694 GN=GMB86_03540 PE=3 SV=1
MM1 pKa = 7.81 PKK3 pKa = 9.43 FTIIDD8 pKa = 3.67 KK9 pKa = 8.11 DD10 pKa = 3.61 TCIACGACGAAAPDD24 pKa = 3.53 IYY26 pKa = 10.99 DD27 pKa = 3.8 YY28 pKa = 11.65 DD29 pKa = 4.89 DD30 pKa = 5.1 DD31 pKa = 6.44 GIAFVLLDD39 pKa = 4.24 KK40 pKa = 10.6 NTGEE44 pKa = 3.81 IGIPEE49 pKa = 4.34 EE50 pKa = 5.39 LIDD53 pKa = 5.61 DD54 pKa = 4.74 MIDD57 pKa = 3.35 AYY59 pKa = 10.1 EE60 pKa = 4.43 GCPTDD65 pKa = 4.01 SVRR68 pKa = 11.84 VSNKK72 pKa = 9.18 PFHH75 pKa = 6.73 GNPDD79 pKa = 3.21 RR80 pKa = 11.84 DD81 pKa = 3.61 EE82 pKa = 4.11
Molecular weight: 8.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.783
IPC_protein 3.77
Toseland 3.541
ProMoST 3.923
Dawson 3.783
Bjellqvist 3.999
Wikipedia 3.757
Rodwell 3.592
Grimsley 3.452
Solomon 3.757
Lehninger 3.719
Nozaki 3.91
DTASelect 4.177
Thurlkill 3.617
EMBOSS 3.757
Sillero 3.897
Patrickios 1.901
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.833
Protein with the highest isoelectric point:
>tr|A0A6N8CPN8|A0A6N8CPN8_9BACI Uncharacterized protein OS=Terrilactibacillus tamarindi OX=2599694 GN=GMB86_08975 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 KK11 pKa = 9.23 RR12 pKa = 11.84 KK13 pKa = 8.02 KK14 pKa = 8.47 VHH16 pKa = 5.56 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MQTKK25 pKa = 9.96 NGRR28 pKa = 11.84 KK29 pKa = 8.74 ILANRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.72 GRR39 pKa = 11.84 KK40 pKa = 8.69 VLSAA44 pKa = 4.05
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.516
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.735
Grimsley 13.013
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.457
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.137
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2956
0
2956
876471
26
1584
296.5
33.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.423 ± 0.041
0.844 ± 0.016
5.309 ± 0.037
6.597 ± 0.05
4.447 ± 0.033
6.722 ± 0.041
2.399 ± 0.022
8.253 ± 0.047
7.371 ± 0.044
9.828 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.707 ± 0.025
4.515 ± 0.035
3.656 ± 0.03
3.873 ± 0.032
4.06 ± 0.033
6.239 ± 0.031
5.487 ± 0.027
6.781 ± 0.036
0.956 ± 0.016
3.533 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here