Salmonella phage ST64B

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8HAB6|Q8HAB6_9CAUD Sb22 OS=Salmonella phage ST64B OX=175950 GN=sb22 PE=4 SV=1
MM1 pKa = 7.78ALQDD5 pKa = 4.33EE6 pKa = 4.86YY7 pKa = 11.42TQLLYY12 pKa = 10.77HH13 pKa = 7.03LLPEE17 pKa = 4.79GPAWDD22 pKa = 4.3GEE24 pKa = 4.31NPLIEE29 pKa = 4.49GLAPSLNRR37 pKa = 11.84VHH39 pKa = 6.38QRR41 pKa = 11.84ADD43 pKa = 3.1EE44 pKa = 4.21LMAEE48 pKa = 4.48IDD50 pKa = 3.65PARR53 pKa = 11.84TTEE56 pKa = 4.62LIDD59 pKa = 5.13RR60 pKa = 11.84YY61 pKa = 9.16EE62 pKa = 3.89QLYY65 pKa = 10.28GLPDD69 pKa = 3.1SCAPEE74 pKa = 4.68GVQTLQQRR82 pKa = 11.84QQRR85 pKa = 11.84LDD87 pKa = 3.15AKK89 pKa = 10.69ANVAGGINEE98 pKa = 3.95RR99 pKa = 11.84FYY101 pKa = 11.24RR102 pKa = 11.84EE103 pKa = 3.69QLDD106 pKa = 3.66ALGYY110 pKa = 7.25TAATIEE116 pKa = 4.12QFQNLDD122 pKa = 3.64STPDD126 pKa = 3.83PEE128 pKa = 4.32WGEE131 pKa = 3.57FWRR134 pKa = 11.84YY135 pKa = 6.3YY136 pKa = 9.12WRR138 pKa = 11.84VNIPADD144 pKa = 4.86ANISWQTCTSTCDD157 pKa = 3.13SAIRR161 pKa = 11.84TWGDD165 pKa = 3.32TVAEE169 pKa = 4.13CVIDD173 pKa = 4.04KK174 pKa = 9.82LCPSHH179 pKa = 5.86TVVVFAYY186 pKa = 9.94PEE188 pKa = 4.36GKK190 pKa = 10.34EE191 pKa = 3.94NAQNN195 pKa = 3.39

Molecular weight: 22.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8HAB2|Q8HAB2_9CAUD Sb26 OS=Salmonella phage ST64B OX=175950 GN=sb26 PE=4 SV=1
MM1 pKa = 7.35FLLYY5 pKa = 10.58CRR7 pKa = 11.84GTGEE11 pKa = 3.79IGGRR15 pKa = 11.84RR16 pKa = 11.84PKK18 pKa = 10.57LSPEE22 pKa = 3.35QWAQAGRR29 pKa = 11.84LIGAGIPRR37 pKa = 11.84QQVAIIYY44 pKa = 9.71DD45 pKa = 3.72VGLSIHH51 pKa = 5.68GLYY54 pKa = 9.9WILLSANRR62 pKa = 11.84KK63 pKa = 7.83GVKK66 pKa = 9.76KK67 pKa = 10.21RR68 pKa = 11.84DD69 pKa = 3.32YY70 pKa = 10.47RR71 pKa = 11.84ANN73 pKa = 3.39

Molecular weight: 8.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

11918

54

642

212.8

23.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.549 ± 0.364

1.191 ± 0.154

5.915 ± 0.303

6.276 ± 0.305

3.541 ± 0.185

7.753 ± 0.312

1.846 ± 0.185

5.185 ± 0.203

5.286 ± 0.274

8.508 ± 0.304

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.609 ± 0.181

4.187 ± 0.306

4.447 ± 0.177

4.195 ± 0.193

6.335 ± 0.338

6.066 ± 0.292

5.706 ± 0.327

6.645 ± 0.201

1.636 ± 0.17

3.121 ± 0.213
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski