Amino acid dipepetide frequency for Salmonella phage ST64B

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.405AlaAla: 10.405 ± 0.918
0.923AlaCys: 0.923 ± 0.283
5.538AlaAsp: 5.538 ± 0.638
5.958AlaGlu: 5.958 ± 0.858
3.86AlaPhe: 3.86 ± 0.726
8.979AlaGly: 8.979 ± 1.007
1.175AlaHis: 1.175 ± 0.312
6.042AlaIle: 6.042 ± 0.611
4.699AlaLys: 4.699 ± 0.559
7.552AlaLeu: 7.552 ± 0.669
2.517AlaMet: 2.517 ± 0.421
3.692AlaAsn: 3.692 ± 0.545
4.28AlaPro: 4.28 ± 0.592
3.273AlaGln: 3.273 ± 0.64
5.706AlaArg: 5.706 ± 0.901
3.944AlaSer: 3.944 ± 0.605
5.37AlaThr: 5.37 ± 0.747
7.3AlaVal: 7.3 ± 0.958
1.594AlaTrp: 1.594 ± 0.316
2.685AlaTyr: 2.685 ± 0.495
0.0AlaXaa: 0.0 ± 0.0
Cys
1.091CysAla: 1.091 ± 0.322
0.252CysCys: 0.252 ± 0.136
0.671CysAsp: 0.671 ± 0.265
0.839CysGlu: 0.839 ± 0.331
0.252CysPhe: 0.252 ± 0.135
0.839CysGly: 0.839 ± 0.222
0.503CysHis: 0.503 ± 0.232
0.252CysIle: 0.252 ± 0.147
0.42CysLys: 0.42 ± 0.189
1.007CysLeu: 1.007 ± 0.295
0.252CysMet: 0.252 ± 0.143
0.168CysAsn: 0.168 ± 0.126
0.671CysPro: 0.671 ± 0.2
0.839CysGln: 0.839 ± 0.266
1.343CysArg: 1.343 ± 0.373
0.42CysSer: 0.42 ± 0.178
0.42CysThr: 0.42 ± 0.18
0.923CysVal: 0.923 ± 0.327
0.336CysTrp: 0.336 ± 0.213
0.42CysTyr: 0.42 ± 0.232
0.0CysXaa: 0.0 ± 0.0
Asp
5.538AspAla: 5.538 ± 0.644
0.755AspCys: 0.755 ± 0.285
5.37AspAsp: 5.37 ± 0.845
2.853AspGlu: 2.853 ± 0.494
2.098AspPhe: 2.098 ± 0.459
5.37AspGly: 5.37 ± 0.68
1.091AspHis: 1.091 ± 0.321
3.189AspIle: 3.189 ± 0.731
2.517AspLys: 2.517 ± 0.494
4.783AspLeu: 4.783 ± 0.587
1.93AspMet: 1.93 ± 0.376
2.35AspAsn: 2.35 ± 0.395
2.853AspPro: 2.853 ± 0.44
1.259AspGln: 1.259 ± 0.297
2.769AspArg: 2.769 ± 0.552
3.776AspSer: 3.776 ± 0.518
2.685AspThr: 2.685 ± 0.454
4.364AspVal: 4.364 ± 0.836
1.175AspTrp: 1.175 ± 0.294
2.433AspTyr: 2.433 ± 0.489
0.0AspXaa: 0.0 ± 0.0
Glu
5.37GluAla: 5.37 ± 0.646
1.091GluCys: 1.091 ± 0.345
3.021GluAsp: 3.021 ± 0.497
3.86GluGlu: 3.86 ± 0.655
2.182GluPhe: 2.182 ± 0.37
3.357GluGly: 3.357 ± 0.507
1.594GluHis: 1.594 ± 0.443
4.112GluIle: 4.112 ± 0.615
3.944GluLys: 3.944 ± 0.786
6.965GluLeu: 6.965 ± 0.85
1.846GluMet: 1.846 ± 0.342
2.685GluAsn: 2.685 ± 0.515
2.098GluPro: 2.098 ± 0.423
3.357GluGln: 3.357 ± 0.531
4.28GluArg: 4.28 ± 0.583
4.364GluSer: 4.364 ± 0.673
3.021GluThr: 3.021 ± 0.473
3.189GluVal: 3.189 ± 0.478
0.923GluTrp: 0.923 ± 0.277
1.51GluTyr: 1.51 ± 0.314
0.0GluXaa: 0.0 ± 0.0
Phe
2.266PheAla: 2.266 ± 0.428
0.168PheCys: 0.168 ± 0.136
2.769PheAsp: 2.769 ± 0.509
2.433PheGlu: 2.433 ± 0.408
1.175PhePhe: 1.175 ± 0.287
3.357PheGly: 3.357 ± 0.558
0.42PheHis: 0.42 ± 0.157
1.93PheIle: 1.93 ± 0.269
1.51PheLys: 1.51 ± 0.368
2.769PheLeu: 2.769 ± 0.434
1.343PheMet: 1.343 ± 0.329
2.182PheAsn: 2.182 ± 0.421
1.259PhePro: 1.259 ± 0.296
1.427PheGln: 1.427 ± 0.239
2.685PheArg: 2.685 ± 0.434
1.594PheSer: 1.594 ± 0.467
2.433PheThr: 2.433 ± 0.431
1.594PheVal: 1.594 ± 0.451
0.755PheTrp: 0.755 ± 0.225
1.343PheTyr: 1.343 ± 0.336
0.0PheXaa: 0.0 ± 0.0
Gly
7.3GlyAla: 7.3 ± 0.982
0.839GlyCys: 0.839 ± 0.287
5.287GlyAsp: 5.287 ± 0.726
5.622GlyGlu: 5.622 ± 0.709
2.937GlyPhe: 2.937 ± 0.426
6.377GlyGly: 6.377 ± 0.803
1.007GlyHis: 1.007 ± 0.296
3.944GlyIle: 3.944 ± 0.546
4.447GlyLys: 4.447 ± 0.548
5.538GlyLeu: 5.538 ± 0.744
2.014GlyMet: 2.014 ± 0.428
4.28GlyAsn: 4.28 ± 0.625
1.594GlyPro: 1.594 ± 0.346
2.685GlyGln: 2.685 ± 0.445
4.783GlyArg: 4.783 ± 0.634
4.28GlySer: 4.28 ± 0.667
4.028GlyThr: 4.028 ± 0.615
6.629GlyVal: 6.629 ± 0.853
1.51GlyTrp: 1.51 ± 0.363
2.433GlyTyr: 2.433 ± 0.414
0.0GlyXaa: 0.0 ± 0.0
His
1.51HisAla: 1.51 ± 0.311
0.252HisCys: 0.252 ± 0.141
1.007HisAsp: 1.007 ± 0.287
1.594HisGlu: 1.594 ± 0.408
0.755HisPhe: 0.755 ± 0.258
1.343HisGly: 1.343 ± 0.317
0.671HisHis: 0.671 ± 0.274
1.594HisIle: 1.594 ± 0.362
0.923HisLys: 0.923 ± 0.269
1.259HisLeu: 1.259 ± 0.275
0.503HisMet: 0.503 ± 0.181
0.839HisAsn: 0.839 ± 0.275
0.671HisPro: 0.671 ± 0.244
0.671HisGln: 0.671 ± 0.236
0.923HisArg: 0.923 ± 0.275
1.259HisSer: 1.259 ± 0.304
1.175HisThr: 1.175 ± 0.275
0.671HisVal: 0.671 ± 0.263
0.084HisTrp: 0.084 ± 0.085
0.755HisTyr: 0.755 ± 0.316
0.0HisXaa: 0.0 ± 0.0
Ile
5.37IleAla: 5.37 ± 0.697
0.587IleCys: 0.587 ± 0.217
3.273IleAsp: 3.273 ± 0.66
4.783IleGlu: 4.783 ± 0.656
0.755IlePhe: 0.755 ± 0.218
4.615IleGly: 4.615 ± 0.715
0.42IleHis: 0.42 ± 0.18
1.93IleIle: 1.93 ± 0.505
2.685IleLys: 2.685 ± 0.517
2.601IleLeu: 2.601 ± 0.427
1.091IleMet: 1.091 ± 0.283
3.021IleAsn: 3.021 ± 0.508
3.273IlePro: 3.273 ± 0.482
1.51IleGln: 1.51 ± 0.316
3.273IleArg: 3.273 ± 0.58
3.776IleSer: 3.776 ± 0.51
4.447IleThr: 4.447 ± 0.505
1.846IleVal: 1.846 ± 0.402
0.671IleTrp: 0.671 ± 0.231
1.93IleTyr: 1.93 ± 0.432
0.0IleXaa: 0.0 ± 0.0
Lys
6.461LysAla: 6.461 ± 0.727
0.252LysCys: 0.252 ± 0.128
2.433LysAsp: 2.433 ± 0.498
3.273LysGlu: 3.273 ± 0.689
1.594LysPhe: 1.594 ± 0.309
4.112LysGly: 4.112 ± 0.577
0.587LysHis: 0.587 ± 0.246
2.685LysIle: 2.685 ± 0.522
3.189LysLys: 3.189 ± 0.576
4.783LysLeu: 4.783 ± 0.725
1.259LysMet: 1.259 ± 0.334
1.762LysAsn: 1.762 ± 0.371
2.35LysPro: 2.35 ± 0.455
3.273LysGln: 3.273 ± 0.541
4.28LysArg: 4.28 ± 0.684
3.524LysSer: 3.524 ± 0.572
2.433LysThr: 2.433 ± 0.394
2.601LysVal: 2.601 ± 0.507
0.503LysTrp: 0.503 ± 0.2
1.51LysTyr: 1.51 ± 0.37
0.0LysXaa: 0.0 ± 0.0
Leu
8.307LeuAla: 8.307 ± 0.806
1.343LeuCys: 1.343 ± 0.421
5.119LeuAsp: 5.119 ± 0.556
5.454LeuGlu: 5.454 ± 0.729
3.357LeuPhe: 3.357 ± 0.44
4.615LeuGly: 4.615 ± 0.667
1.594LeuHis: 1.594 ± 0.382
3.944LeuIle: 3.944 ± 0.536
5.538LeuLys: 5.538 ± 0.839
6.797LeuLeu: 6.797 ± 0.846
2.014LeuMet: 2.014 ± 0.352
3.86LeuAsn: 3.86 ± 0.559
4.196LeuPro: 4.196 ± 0.571
3.021LeuGln: 3.021 ± 0.45
5.706LeuArg: 5.706 ± 0.595
5.706LeuSer: 5.706 ± 0.814
4.867LeuThr: 4.867 ± 0.736
5.454LeuVal: 5.454 ± 0.779
1.259LeuTrp: 1.259 ± 0.354
2.937LeuTyr: 2.937 ± 0.542
0.0LeuXaa: 0.0 ± 0.0
Met
2.685MetAla: 2.685 ± 0.644
0.252MetCys: 0.252 ± 0.134
0.755MetAsp: 0.755 ± 0.242
1.343MetGlu: 1.343 ± 0.387
0.923MetPhe: 0.923 ± 0.32
1.678MetGly: 1.678 ± 0.407
0.671MetHis: 0.671 ± 0.221
0.923MetIle: 0.923 ± 0.297
1.427MetLys: 1.427 ± 0.298
2.853MetLeu: 2.853 ± 0.508
0.168MetMet: 0.168 ± 0.108
1.678MetAsn: 1.678 ± 0.338
1.259MetPro: 1.259 ± 0.291
1.51MetGln: 1.51 ± 0.466
1.427MetArg: 1.427 ± 0.372
2.433MetSer: 2.433 ± 0.43
1.762MetThr: 1.762 ± 0.351
1.259MetVal: 1.259 ± 0.309
0.587MetTrp: 0.587 ± 0.182
0.503MetTyr: 0.503 ± 0.216
0.0MetXaa: 0.0 ± 0.0
Asn
4.447AsnAla: 4.447 ± 0.727
0.336AsnCys: 0.336 ± 0.159
2.182AsnAsp: 2.182 ± 0.596
2.517AsnGlu: 2.517 ± 0.377
1.594AsnPhe: 1.594 ± 0.374
4.615AsnGly: 4.615 ± 0.93
0.587AsnHis: 0.587 ± 0.204
2.517AsnIle: 2.517 ± 0.454
2.098AsnLys: 2.098 ± 0.493
2.517AsnLeu: 2.517 ± 0.495
1.007AsnMet: 1.007 ± 0.287
2.266AsnAsn: 2.266 ± 0.557
2.685AsnPro: 2.685 ± 0.517
1.762AsnGln: 1.762 ± 0.379
2.266AsnArg: 2.266 ± 0.456
2.937AsnSer: 2.937 ± 0.703
1.846AsnThr: 1.846 ± 0.368
1.846AsnVal: 1.846 ± 0.381
0.336AsnTrp: 0.336 ± 0.15
1.51AsnTyr: 1.51 ± 0.291
0.0AsnXaa: 0.0 ± 0.0
Pro
4.867ProAla: 4.867 ± 0.627
0.671ProCys: 0.671 ± 0.255
3.105ProAsp: 3.105 ± 0.553
4.196ProGlu: 4.196 ± 0.644
1.427ProPhe: 1.427 ± 0.452
3.692ProGly: 3.692 ± 0.54
0.923ProHis: 0.923 ± 0.302
1.343ProIle: 1.343 ± 0.324
2.182ProLys: 2.182 ± 0.382
4.028ProLeu: 4.028 ± 0.52
0.839ProMet: 0.839 ± 0.267
1.51ProAsn: 1.51 ± 0.356
1.427ProPro: 1.427 ± 0.352
2.014ProGln: 2.014 ± 0.395
2.182ProArg: 2.182 ± 0.44
2.433ProSer: 2.433 ± 0.361
1.594ProThr: 1.594 ± 0.305
4.196ProVal: 4.196 ± 0.663
0.336ProTrp: 0.336 ± 0.143
1.51ProTyr: 1.51 ± 0.367
0.0ProXaa: 0.0 ± 0.0
Gln
3.86GlnAla: 3.86 ± 0.485
0.42GlnCys: 0.42 ± 0.17
1.51GlnAsp: 1.51 ± 0.376
2.098GlnGlu: 2.098 ± 0.498
1.762GlnPhe: 1.762 ± 0.354
2.266GlnGly: 2.266 ± 0.477
1.091GlnHis: 1.091 ± 0.261
1.846GlnIle: 1.846 ± 0.538
3.357GlnLys: 3.357 ± 0.545
4.028GlnLeu: 4.028 ± 0.548
1.175GlnMet: 1.175 ± 0.267
1.93GlnAsn: 1.93 ± 0.466
2.266GlnPro: 2.266 ± 0.372
2.769GlnGln: 2.769 ± 0.579
3.189GlnArg: 3.189 ± 0.576
2.182GlnSer: 2.182 ± 0.51
2.182GlnThr: 2.182 ± 0.44
2.098GlnVal: 2.098 ± 0.468
0.755GlnTrp: 0.755 ± 0.202
1.175GlnTyr: 1.175 ± 0.279
0.0GlnXaa: 0.0 ± 0.0
Arg
5.119ArgAla: 5.119 ± 0.586
0.755ArgCys: 0.755 ± 0.255
4.112ArgAsp: 4.112 ± 0.686
3.021ArgGlu: 3.021 ± 0.469
2.937ArgPhe: 2.937 ± 0.628
3.86ArgGly: 3.86 ± 0.659
1.846ArgHis: 1.846 ± 0.383
2.937ArgIle: 2.937 ± 0.403
4.531ArgLys: 4.531 ± 0.803
5.874ArgLeu: 5.874 ± 0.675
2.182ArgMet: 2.182 ± 0.385
1.93ArgAsn: 1.93 ± 0.397
2.601ArgPro: 2.601 ± 0.508
2.433ArgGln: 2.433 ± 0.54
4.447ArgArg: 4.447 ± 0.718
3.189ArgSer: 3.189 ± 0.57
3.692ArgThr: 3.692 ± 0.504
4.531ArgVal: 4.531 ± 0.748
0.839ArgTrp: 0.839 ± 0.272
2.517ArgTyr: 2.517 ± 0.503
0.0ArgXaa: 0.0 ± 0.0
Ser
5.706SerAla: 5.706 ± 0.725
0.839SerCys: 0.839 ± 0.285
3.944SerAsp: 3.944 ± 0.56
3.105SerGlu: 3.105 ± 0.511
1.51SerPhe: 1.51 ± 0.395
5.622SerGly: 5.622 ± 0.83
1.427SerHis: 1.427 ± 0.342
2.853SerIle: 2.853 ± 0.45
1.846SerLys: 1.846 ± 0.409
5.706SerLeu: 5.706 ± 0.825
2.098SerMet: 2.098 ± 0.38
2.014SerAsn: 2.014 ± 0.335
2.35SerPro: 2.35 ± 0.424
3.105SerGln: 3.105 ± 0.478
3.273SerArg: 3.273 ± 0.53
3.105SerSer: 3.105 ± 0.549
3.44SerThr: 3.44 ± 0.715
5.958SerVal: 5.958 ± 0.672
1.259SerTrp: 1.259 ± 0.408
1.427SerTyr: 1.427 ± 0.376
0.0SerXaa: 0.0 ± 0.0
Thr
5.874ThrAla: 5.874 ± 0.78
0.671ThrCys: 0.671 ± 0.262
3.105ThrAsp: 3.105 ± 0.451
3.44ThrGlu: 3.44 ± 0.497
1.93ThrPhe: 1.93 ± 0.383
5.37ThrGly: 5.37 ± 0.736
0.671ThrHis: 0.671 ± 0.203
3.105ThrIle: 3.105 ± 0.418
2.014ThrLys: 2.014 ± 0.416
5.287ThrLeu: 5.287 ± 0.624
1.343ThrMet: 1.343 ± 0.292
1.175ThrAsn: 1.175 ± 0.321
2.769ThrPro: 2.769 ± 0.504
2.098ThrGln: 2.098 ± 0.336
2.937ThrArg: 2.937 ± 0.439
4.364ThrSer: 4.364 ± 0.619
4.028ThrThr: 4.028 ± 0.576
4.028ThrVal: 4.028 ± 0.676
1.007ThrTrp: 1.007 ± 0.297
1.846ThrTyr: 1.846 ± 0.374
0.0ThrXaa: 0.0 ± 0.0
Val
5.958ValAla: 5.958 ± 0.873
0.587ValCys: 0.587 ± 0.206
3.776ValAsp: 3.776 ± 0.606
3.692ValGlu: 3.692 ± 0.576
3.021ValPhe: 3.021 ± 0.455
3.608ValGly: 3.608 ± 0.566
1.007ValHis: 1.007 ± 0.452
4.531ValIle: 4.531 ± 0.595
3.776ValLys: 3.776 ± 0.618
6.294ValLeu: 6.294 ± 0.643
1.678ValMet: 1.678 ± 0.362
3.189ValAsn: 3.189 ± 0.578
3.524ValPro: 3.524 ± 0.495
2.601ValGln: 2.601 ± 0.422
4.112ValArg: 4.112 ± 0.569
3.692ValSer: 3.692 ± 0.545
4.447ValThr: 4.447 ± 0.634
4.196ValVal: 4.196 ± 0.664
1.091ValTrp: 1.091 ± 0.267
1.678ValTyr: 1.678 ± 0.421
0.0ValXaa: 0.0 ± 0.0
Trp
0.839TrpAla: 0.839 ± 0.312
0.42TrpCys: 0.42 ± 0.179
0.587TrpAsp: 0.587 ± 0.185
0.923TrpGlu: 0.923 ± 0.29
0.42TrpPhe: 0.42 ± 0.175
1.343TrpGly: 1.343 ± 0.342
0.503TrpHis: 0.503 ± 0.232
0.755TrpIle: 0.755 ± 0.251
0.755TrpLys: 0.755 ± 0.219
1.846TrpLeu: 1.846 ± 0.404
0.336TrpMet: 0.336 ± 0.173
0.42TrpAsn: 0.42 ± 0.177
1.007TrpPro: 1.007 ± 0.3
0.671TrpGln: 0.671 ± 0.228
1.594TrpArg: 1.594 ± 0.415
0.923TrpSer: 0.923 ± 0.249
0.755TrpThr: 0.755 ± 0.218
1.343TrpVal: 1.343 ± 0.375
0.42TrpTrp: 0.42 ± 0.197
0.503TrpTyr: 0.503 ± 0.208
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.517TyrAla: 2.517 ± 0.443
0.503TyrCys: 0.503 ± 0.216
1.594TyrAsp: 1.594 ± 0.384
1.762TyrGlu: 1.762 ± 0.393
0.923TyrPhe: 0.923 ± 0.276
2.517TyrGly: 2.517 ± 0.512
0.671TyrHis: 0.671 ± 0.199
1.427TyrIle: 1.427 ± 0.364
1.007TyrLys: 1.007 ± 0.281
2.601TyrLeu: 2.601 ± 0.55
0.42TyrMet: 0.42 ± 0.178
0.923TyrAsn: 0.923 ± 0.235
1.343TyrPro: 1.343 ± 0.336
1.678TyrGln: 1.678 ± 0.355
2.182TyrArg: 2.182 ± 0.408
2.769TyrSer: 2.769 ± 0.526
2.433TyrThr: 2.433 ± 0.515
2.433TyrVal: 2.433 ± 0.566
0.923TyrTrp: 0.923 ± 0.26
0.587TyrTyr: 0.587 ± 0.217
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (11918 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski