Halodesulfovibrio spirochaetisodalis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfovibrionales; Desulfovibrionaceae; Halodesulfovibrio

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2803 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B7XAI0|A0A1B7XAI0_9DELT Uncharacterized protein OS=Halodesulfovibrio spirochaetisodalis OX=1560234 GN=SP90_12790 PE=4 SV=1
GG1 pKa = 7.65EE2 pKa = 5.96DD3 pKa = 3.66GDD5 pKa = 4.42DD6 pKa = 4.12TITTTGSAYY15 pKa = 9.46QHH17 pKa = 5.19VQGGNGNDD25 pKa = 4.04TIRR28 pKa = 11.84TGSANDD34 pKa = 4.48AIIGGADD41 pKa = 4.09NDD43 pKa = 4.82TIWAGEE49 pKa = 4.09GDD51 pKa = 3.52NSIWGGTGEE60 pKa = 4.96DD61 pKa = 4.48EE62 pKa = 4.19IHH64 pKa = 6.6SGSGNDD70 pKa = 4.14TIDD73 pKa = 4.35AGDD76 pKa = 4.47DD77 pKa = 3.68NDD79 pKa = 5.1TIWAGDD85 pKa = 3.56GTNTIDD91 pKa = 3.81AGKK94 pKa = 10.76GNDD97 pKa = 4.1IIHH100 pKa = 7.0AGANADD106 pKa = 3.57TYY108 pKa = 11.02KK109 pKa = 10.58YY110 pKa = 10.95SKK112 pKa = 11.21GDD114 pKa = 3.46GHH116 pKa = 7.13DD117 pKa = 3.46VIYY120 pKa = 10.73DD121 pKa = 3.74NEE123 pKa = 4.57SGAVTADD130 pKa = 3.21HH131 pKa = 6.53TLDD134 pKa = 4.58AIRR137 pKa = 11.84FSSDD141 pKa = 2.07IAYY144 pKa = 10.51NDD146 pKa = 3.5LQFAIDD152 pKa = 4.68GNDD155 pKa = 3.6LLITFKK161 pKa = 11.0NSPEE165 pKa = 3.59DD166 pKa = 3.45SIRR169 pKa = 11.84IKK171 pKa = 10.81DD172 pKa = 3.28AATNPEE178 pKa = 4.0HH179 pKa = 6.91AVEE182 pKa = 5.47KK183 pKa = 10.6ILIEE187 pKa = 3.94NRR189 pKa = 11.84YY190 pKa = 8.97NASQVSTYY198 pKa = 9.95YY199 pKa = 10.69VEE201 pKa = 4.15NAAIRR206 pKa = 11.84HH207 pKa = 4.49TTYY210 pKa = 11.29SDD212 pKa = 3.51GDD214 pKa = 3.77DD215 pKa = 4.09TISVPVSSKK224 pKa = 8.1ITKK227 pKa = 10.13ISAGDD232 pKa = 3.66GNDD235 pKa = 3.18TVTSGDD241 pKa = 3.34SSVIIDD247 pKa = 4.1GGAGNDD253 pKa = 4.0TITTGSKK260 pKa = 10.43SDD262 pKa = 3.86VISGGTGNDD271 pKa = 3.73TINAGGGSDD280 pKa = 3.56TLTGGSGSDD289 pKa = 3.06TYY291 pKa = 11.11IFNIGDD297 pKa = 3.49GHH299 pKa = 5.95DD300 pKa = 4.03TIIEE304 pKa = 4.03ASVDD308 pKa = 3.81PTCSCVDD315 pKa = 4.07TISFGAGITSSDD327 pKa = 3.43IIYY330 pKa = 10.55KK331 pKa = 10.12VDD333 pKa = 3.53GNDD336 pKa = 4.95LIICIKK342 pKa = 10.55GHH344 pKa = 6.98DD345 pKa = 4.08GDD347 pKa = 4.67SIRR350 pKa = 11.84ITGALNGASHH360 pKa = 7.45GIEE363 pKa = 4.16TITFASGTTITLNTVISCHH382 pKa = 6.29NYY384 pKa = 8.89TDD386 pKa = 3.74ASDD389 pKa = 4.21TIVVPADD396 pKa = 3.36KK397 pKa = 10.91YY398 pKa = 10.64VVVINAGGGNDD409 pKa = 4.27TITGGDD415 pKa = 3.31TVSIINGGTGNDD427 pKa = 4.02SISTGGKK434 pKa = 10.02DD435 pKa = 3.28DD436 pKa = 3.92TLTGGEE442 pKa = 4.35GDD444 pKa = 3.72DD445 pKa = 4.49TINAGGGNDD454 pKa = 4.25TITGGTGSDD463 pKa = 3.41TLTGGSGSDD472 pKa = 3.06TYY474 pKa = 11.11IFNIGDD480 pKa = 3.49GHH482 pKa = 5.95DD483 pKa = 4.03TIIEE487 pKa = 4.03ASVDD491 pKa = 3.74PTCTCVDD498 pKa = 4.4TISFGAGITSSDD510 pKa = 3.43IIYY513 pKa = 10.55KK514 pKa = 10.12VDD516 pKa = 3.53GNDD519 pKa = 4.95LIICIKK525 pKa = 10.55GHH527 pKa = 6.98DD528 pKa = 4.08GDD530 pKa = 4.67SIRR533 pKa = 11.84ITGALNGASHH543 pKa = 7.45GIEE546 pKa = 4.16TITFASGTSITLNTVISCHH565 pKa = 6.29NYY567 pKa = 8.89TDD569 pKa = 3.74ASDD572 pKa = 4.21TIVVPADD579 pKa = 3.36KK580 pKa = 10.91YY581 pKa = 10.64VVVINAGGGNDD592 pKa = 4.27TITGGDD598 pKa = 3.31TVSIINGGTGNDD610 pKa = 4.02SISTGGKK617 pKa = 10.04DD618 pKa = 4.33DD619 pKa = 3.92ILTGGEE625 pKa = 4.02GDD627 pKa = 3.7DD628 pKa = 4.49TINAGGGNDD637 pKa = 4.13IITGGTGSDD646 pKa = 3.41TLTGGSGSDD655 pKa = 3.06TYY657 pKa = 11.11IFNIGDD663 pKa = 3.49GHH665 pKa = 5.95DD666 pKa = 4.03TIIEE670 pKa = 4.03ASVDD674 pKa = 3.81PTCSCVDD681 pKa = 4.07TISFGAGITSSDD693 pKa = 3.43IIYY696 pKa = 10.55KK697 pKa = 10.12VDD699 pKa = 3.53GNDD702 pKa = 4.95LIICIKK708 pKa = 10.55GHH710 pKa = 6.98DD711 pKa = 4.08GDD713 pKa = 4.67SIRR716 pKa = 11.84ITGALNGTSSFGVEE730 pKa = 4.15NISFTDD736 pKa = 3.87GTSIALTTVISCQNYY751 pKa = 8.51TDD753 pKa = 3.92ASDD756 pKa = 4.2TIVVPADD763 pKa = 3.34KK764 pKa = 10.77YY765 pKa = 10.03VAVINAGGGNDD776 pKa = 4.27TITGGDD782 pKa = 3.28TASIINGGAGNDD794 pKa = 4.16SISTGAKK801 pKa = 10.11DD802 pKa = 4.98DD803 pKa = 3.95ILNGGIGNDD812 pKa = 3.95TINAGGGNDD821 pKa = 4.25TITGGTGTDD830 pKa = 3.48TLIGGSGSDD839 pKa = 3.12TYY841 pKa = 11.11IFNIGDD847 pKa = 3.49GHH849 pKa = 5.95DD850 pKa = 4.03TIIEE854 pKa = 4.03ASVDD858 pKa = 3.81PTCSCVDD865 pKa = 4.36KK866 pKa = 10.74IQLGVGIGIEE876 pKa = 4.03DD877 pKa = 3.72LRR879 pKa = 11.84YY880 pKa = 10.21SADD883 pKa = 3.54GDD885 pKa = 3.85DD886 pKa = 5.36LIINFRR892 pKa = 11.84GNDD895 pKa = 3.29SDD897 pKa = 4.26SIRR900 pKa = 11.84IVGAKK905 pKa = 8.81TGASAGIEE913 pKa = 4.38SICFADD919 pKa = 4.29GTQMEE924 pKa = 4.61ISSAVTIIEE933 pKa = 4.13LTEE936 pKa = 4.13NMDD939 pKa = 3.99FQMMPVPAQTAIINGYY955 pKa = 9.08GGNDD959 pKa = 4.64FINVTGATNCIVDD972 pKa = 4.04GGSGIDD978 pKa = 3.68QIMSTAANSILYY990 pKa = 10.16GGADD994 pKa = 3.86MDD996 pKa = 4.11MLSAVNGKK1004 pKa = 8.49ATFIGGTGNDD1014 pKa = 4.07TLTGGYY1020 pKa = 10.35LGDD1023 pKa = 3.68TYY1025 pKa = 11.7VFAQGDD1031 pKa = 4.1GQDD1034 pKa = 3.98IINDD1038 pKa = 4.18NVPMTCVANFDD1049 pKa = 4.11PDD1051 pKa = 4.31TIKK1054 pKa = 10.85LGNGITKK1061 pKa = 10.35NDD1063 pKa = 2.99VAFFMEE1069 pKa = 5.16GPNLIVSYY1077 pKa = 11.26GEE1079 pKa = 3.83TDD1081 pKa = 2.99RR1082 pKa = 11.84VTVLGQANAKK1092 pKa = 9.58NAIEE1096 pKa = 4.18TVQLASGSSLSSAEE1110 pKa = 3.79INQIVADD1117 pKa = 4.46LSNYY1121 pKa = 10.36ASDD1124 pKa = 3.86HH1125 pKa = 5.98GLDD1128 pKa = 3.68FTSVEE1133 pKa = 4.14DD1134 pKa = 3.53VKK1136 pKa = 11.55NNQEE1140 pKa = 3.88LMNIVTAAWDD1150 pKa = 3.43NN1151 pKa = 3.6

Molecular weight: 116.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B7XFL4|A0A1B7XFL4_9DELT tRNA modification GTPase MnmE OS=Halodesulfovibrio spirochaetisodalis OX=1560234 GN=mnmE PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.17QPSKK9 pKa = 9.44IKK11 pKa = 10.44RR12 pKa = 11.84KK13 pKa = 7.76RR14 pKa = 11.84THH16 pKa = 6.05GFRR19 pKa = 11.84ARR21 pKa = 11.84LKK23 pKa = 8.16TASGRR28 pKa = 11.84AILRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.48GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2803

0

2803

889119

44

3318

317.2

35.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.121 ± 0.048

1.391 ± 0.023

5.304 ± 0.038

6.752 ± 0.047

4.166 ± 0.034

7.311 ± 0.053

2.131 ± 0.021

6.282 ± 0.037

5.498 ± 0.046

9.887 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.845 ± 0.021

3.683 ± 0.03

4.194 ± 0.028

3.468 ± 0.03

4.781 ± 0.039

6.157 ± 0.032

5.775 ± 0.04

7.315 ± 0.04

1.076 ± 0.018

2.861 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski