Staphylococcus aureus

Taxonomy:

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6JQF8|A0A1W6JQF8_STAAU Pathogenicity island protein OS=Staphylococcus aureus OX=1280 PE=4 SV=1
MM1 pKa = 7.85ASQLFEE7 pKa = 5.36DD8 pKa = 4.86EE9 pKa = 4.28YY10 pKa = 11.57FMYY13 pKa = 10.87GLQTYY18 pKa = 10.27AEE20 pKa = 4.6SNNSDD25 pKa = 3.1VFEE28 pKa = 4.03YY29 pKa = 11.02LEE31 pKa = 4.58NGFDD35 pKa = 3.74TDD37 pKa = 3.69TLEE40 pKa = 5.82GIQSSNTDD48 pKa = 3.21VIANIEE54 pKa = 4.17MLYY57 pKa = 10.52QLATGINEE65 pKa = 4.14PATEE69 pKa = 3.94LVEE72 pKa = 4.14GLKK75 pKa = 10.73LVTEE79 pKa = 5.05FVQDD83 pKa = 3.54EE84 pKa = 4.36NATQEE89 pKa = 4.13DD90 pKa = 4.96YY91 pKa = 11.18KK92 pKa = 11.48ALEE95 pKa = 4.34RR96 pKa = 11.84KK97 pKa = 10.2LNDD100 pKa = 3.62LKK102 pKa = 11.21ASYY105 pKa = 10.83YY106 pKa = 10.56SLSKK110 pKa = 10.98

Molecular weight: 12.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6JQI7|A0A1W6JQI7_STAAU Pathogenicity island protein OS=Staphylococcus aureus OX=1280 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.27LSKK5 pKa = 10.48SKK7 pKa = 10.96NVLYY11 pKa = 10.52YY12 pKa = 10.9RR13 pKa = 11.84NVDD16 pKa = 3.42NKK18 pKa = 10.66LSEE21 pKa = 4.36YY22 pKa = 10.76QLLTQFNPAFINKK35 pKa = 8.6KK36 pKa = 9.68IKK38 pKa = 9.14MCEE41 pKa = 3.79FQIEE45 pKa = 4.63SMYY48 pKa = 11.07HH49 pKa = 4.98MSASTTTCDD58 pKa = 3.11EE59 pKa = 3.78IMGVVSVSYY68 pKa = 9.82PIEE71 pKa = 4.11KK72 pKa = 10.29LVIKK76 pKa = 10.38IIEE79 pKa = 4.41TKK81 pKa = 10.74AGLQTYY87 pKa = 10.16KK88 pKa = 10.63NRR90 pKa = 11.84SINNMALLKK99 pKa = 10.5KK100 pKa = 10.23VLNHH104 pKa = 5.01YY105 pKa = 7.29TEE107 pKa = 5.18KK108 pKa = 10.55EE109 pKa = 3.81QKK111 pKa = 10.14QVVKK115 pKa = 10.31YY116 pKa = 8.17MRR118 pKa = 11.84SNGRR122 pKa = 11.84YY123 pKa = 8.61KK124 pKa = 10.23PYY126 pKa = 10.97NVIEE130 pKa = 4.13RR131 pKa = 11.84LQVDD135 pKa = 5.23LYY137 pKa = 10.41QASIKK142 pKa = 9.94QRR144 pKa = 11.84SEE146 pKa = 3.63RR147 pKa = 11.84QKK149 pKa = 10.67QRR151 pKa = 11.84NTAIEE156 pKa = 3.83NSKK159 pKa = 9.63IARR162 pKa = 11.84VNAYY166 pKa = 9.01HH167 pKa = 6.0QSSYY171 pKa = 10.74VKK173 pKa = 10.67VVV175 pKa = 2.74

Molecular weight: 20.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20

0

20

3279

58

489

163.9

19.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.788 ± 0.384

0.823 ± 0.153

6.679 ± 0.521

8.387 ± 0.651

3.843 ± 0.425

4.056 ± 0.388

2.47 ± 0.219

7.777 ± 0.307

10.369 ± 0.59

7.838 ± 0.427

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.287 ± 0.293

6.923 ± 0.64

2.196 ± 0.282

3.66 ± 0.358

3.904 ± 0.464

6.404 ± 0.529

5.306 ± 0.472

6.282 ± 0.457

1.037 ± 0.228

4.971 ± 0.362
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski