Amino acid dipepetide frequency for Staphylococcus aureus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.61AlaAla: 0.61 ± 0.427
0.305AlaCys: 0.305 ± 0.339
2.746AlaAsp: 2.746 ± 0.878
3.356AlaGlu: 3.356 ± 0.986
2.441AlaPhe: 2.441 ± 0.967
1.83AlaGly: 1.83 ± 0.755
1.83AlaHis: 1.83 ± 0.8
3.051AlaIle: 3.051 ± 0.867
3.051AlaLys: 3.051 ± 0.933
5.796AlaLeu: 5.796 ± 1.128
0.61AlaMet: 0.61 ± 0.529
4.576AlaAsn: 4.576 ± 1.165
0.915AlaPro: 0.915 ± 0.681
0.915AlaGln: 0.915 ± 0.502
3.661AlaArg: 3.661 ± 1.658
4.576AlaSer: 4.576 ± 1.275
2.135AlaThr: 2.135 ± 1.172
1.83AlaVal: 1.83 ± 0.67
0.915AlaTrp: 0.915 ± 0.594
2.746AlaTyr: 2.746 ± 0.761
0.0AlaXaa: 0.0 ± 0.0
Cys
0.305CysAla: 0.305 ± 0.279
0.0CysCys: 0.0 ± 0.0
0.61CysAsp: 0.61 ± 0.456
1.22CysGlu: 1.22 ± 0.578
0.61CysPhe: 0.61 ± 0.439
0.305CysGly: 0.305 ± 0.255
0.0CysHis: 0.0 ± 0.0
0.305CysIle: 0.305 ± 0.31
0.915CysLys: 0.915 ± 0.492
0.305CysLeu: 0.305 ± 0.339
0.305CysMet: 0.305 ± 0.31
0.915CysAsn: 0.915 ± 0.509
0.305CysPro: 0.305 ± 0.278
0.0CysGln: 0.0 ± 0.0
0.305CysArg: 0.305 ± 0.311
0.915CysSer: 0.915 ± 0.485
0.305CysThr: 0.305 ± 0.279
0.61CysVal: 0.61 ± 0.438
0.0CysTrp: 0.0 ± 0.0
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
3.356AspAla: 3.356 ± 1.469
0.61AspCys: 0.61 ± 0.355
2.746AspAsp: 2.746 ± 1.17
8.847AspGlu: 8.847 ± 2.034
1.83AspPhe: 1.83 ± 0.68
2.441AspGly: 2.441 ± 0.861
1.525AspHis: 1.525 ± 0.62
5.186AspIle: 5.186 ± 0.932
7.627AspLys: 7.627 ± 2.036
4.271AspLeu: 4.271 ± 0.788
1.525AspMet: 1.525 ± 0.534
3.051AspAsn: 3.051 ± 1.147
1.525AspPro: 1.525 ± 0.463
1.83AspGln: 1.83 ± 0.839
0.915AspArg: 0.915 ± 0.538
4.576AspSer: 4.576 ± 1.354
3.966AspThr: 3.966 ± 1.192
6.101AspVal: 6.101 ± 1.27
0.61AspTrp: 0.61 ± 0.335
4.271AspTyr: 4.271 ± 1.289
0.0AspXaa: 0.0 ± 0.0
Glu
5.796GluAla: 5.796 ± 1.278
0.61GluCys: 0.61 ± 0.469
4.881GluAsp: 4.881 ± 1.06
4.881GluGlu: 4.881 ± 1.191
4.881GluPhe: 4.881 ± 1.45
3.966GluGly: 3.966 ± 1.061
1.83GluHis: 1.83 ± 0.659
7.322GluIle: 7.322 ± 1.043
7.322GluLys: 7.322 ± 1.239
8.542GluLeu: 8.542 ± 1.954
2.746GluMet: 2.746 ± 0.816
4.271GluAsn: 4.271 ± 1.031
0.61GluPro: 0.61 ± 0.498
3.356GluGln: 3.356 ± 1.128
3.966GluArg: 3.966 ± 0.934
3.051GluSer: 3.051 ± 0.681
5.186GluThr: 5.186 ± 1.32
6.101GluVal: 6.101 ± 1.548
0.915GluTrp: 0.915 ± 0.535
3.661GluTyr: 3.661 ± 1.013
0.0GluXaa: 0.0 ± 0.0
Phe
0.915PheAla: 0.915 ± 0.439
0.305PheCys: 0.305 ± 0.279
3.356PheAsp: 3.356 ± 1.273
5.796PheGlu: 5.796 ± 1.2
1.22PhePhe: 1.22 ± 0.602
1.525PheGly: 1.525 ± 0.641
1.22PheHis: 1.22 ± 0.605
1.83PheIle: 1.83 ± 0.612
3.356PheLys: 3.356 ± 1.206
2.746PheLeu: 2.746 ± 0.832
1.22PheMet: 1.22 ± 0.836
3.051PheAsn: 3.051 ± 1.208
0.61PhePro: 0.61 ± 0.361
1.83PheGln: 1.83 ± 0.611
1.22PheArg: 1.22 ± 0.527
2.441PheSer: 2.441 ± 0.764
1.22PheThr: 1.22 ± 0.569
2.746PheVal: 2.746 ± 0.804
0.61PheTrp: 0.61 ± 0.372
1.22PheTyr: 1.22 ± 0.654
0.0PheXaa: 0.0 ± 0.0
Gly
1.525GlyAla: 1.525 ± 0.823
0.61GlyCys: 0.61 ± 0.427
3.356GlyAsp: 3.356 ± 0.829
3.661GlyGlu: 3.661 ± 0.643
1.83GlyPhe: 1.83 ± 0.61
1.22GlyGly: 1.22 ± 0.564
1.83GlyHis: 1.83 ± 0.874
3.356GlyIle: 3.356 ± 1.294
3.661GlyLys: 3.661 ± 1.019
4.576GlyLeu: 4.576 ± 1.318
1.22GlyMet: 1.22 ± 0.629
2.135GlyAsn: 2.135 ± 0.462
0.0GlyPro: 0.0 ± 0.0
1.22GlyGln: 1.22 ± 0.593
0.915GlyArg: 0.915 ± 0.454
2.135GlySer: 2.135 ± 0.758
1.525GlyThr: 1.525 ± 0.553
2.441GlyVal: 2.441 ± 0.795
0.61GlyTrp: 0.61 ± 0.476
2.746GlyTyr: 2.746 ± 1.091
0.0GlyXaa: 0.0 ± 0.0
His
0.915HisAla: 0.915 ± 0.643
0.305HisCys: 0.305 ± 0.279
1.22HisAsp: 1.22 ± 0.533
1.525HisGlu: 1.525 ± 0.678
0.61HisPhe: 0.61 ± 0.373
0.0HisGly: 0.0 ± 0.0
1.525HisHis: 1.525 ± 0.868
1.83HisIle: 1.83 ± 0.43
2.135HisLys: 2.135 ± 0.749
0.915HisLeu: 0.915 ± 0.488
0.61HisMet: 0.61 ± 0.462
1.22HisAsn: 1.22 ± 0.677
0.915HisPro: 0.915 ± 0.624
0.305HisGln: 0.305 ± 0.281
1.83HisArg: 1.83 ± 0.674
1.525HisSer: 1.525 ± 0.814
1.83HisThr: 1.83 ± 0.642
2.746HisVal: 2.746 ± 1.092
0.61HisTrp: 0.61 ± 0.438
2.135HisTyr: 2.135 ± 0.594
0.0HisXaa: 0.0 ± 0.0
Ile
2.746IleAla: 2.746 ± 1.131
0.61IleCys: 0.61 ± 0.438
6.406IleAsp: 6.406 ± 1.479
10.067IleGlu: 10.067 ± 1.877
1.525IlePhe: 1.525 ± 0.497
2.746IleGly: 2.746 ± 0.529
1.22IleHis: 1.22 ± 0.723
3.661IleIle: 3.661 ± 0.864
12.508IleLys: 12.508 ± 1.475
3.661IleLeu: 3.661 ± 1.33
0.61IleMet: 0.61 ± 0.482
7.627IleAsn: 7.627 ± 1.436
2.135IlePro: 2.135 ± 0.671
3.051IleGln: 3.051 ± 0.791
2.441IleArg: 2.441 ± 0.668
6.406IleSer: 6.406 ± 1.491
4.271IleThr: 4.271 ± 1.655
3.966IleVal: 3.966 ± 1.281
0.305IleTrp: 0.305 ± 0.255
1.83IleTyr: 1.83 ± 0.62
0.0IleXaa: 0.0 ± 0.0
Lys
5.796LysAla: 5.796 ± 1.173
0.0LysCys: 0.0 ± 0.0
8.542LysAsp: 8.542 ± 1.811
7.322LysGlu: 7.322 ± 1.466
2.135LysPhe: 2.135 ± 0.722
4.271LysGly: 4.271 ± 1.282
1.83LysHis: 1.83 ± 0.707
6.711LysIle: 6.711 ± 1.377
10.982LysLys: 10.982 ± 1.904
10.677LysLeu: 10.677 ± 1.42
1.83LysMet: 1.83 ± 0.622
5.186LysAsn: 5.186 ± 1.69
2.746LysPro: 2.746 ± 1.066
5.491LysGln: 5.491 ± 1.324
4.881LysArg: 4.881 ± 1.143
5.491LysSer: 5.491 ± 1.895
7.016LysThr: 7.016 ± 1.881
6.406LysVal: 6.406 ± 1.0
0.915LysTrp: 0.915 ± 0.395
5.186LysTyr: 5.186 ± 1.016
0.0LysXaa: 0.0 ± 0.0
Leu
3.356LeuAla: 3.356 ± 0.985
0.915LeuCys: 0.915 ± 0.567
4.576LeuAsp: 4.576 ± 1.48
9.457LeuGlu: 9.457 ± 1.743
3.966LeuPhe: 3.966 ± 0.903
1.83LeuGly: 1.83 ± 0.592
0.915LeuHis: 0.915 ± 0.516
5.796LeuIle: 5.796 ± 1.161
10.067LeuLys: 10.067 ± 1.684
7.016LeuLeu: 7.016 ± 1.49
2.135LeuMet: 2.135 ± 0.992
3.661LeuAsn: 3.661 ± 0.985
2.135LeuPro: 2.135 ± 0.785
2.135LeuGln: 2.135 ± 0.712
2.135LeuArg: 2.135 ± 0.899
4.881LeuSer: 4.881 ± 1.357
3.966LeuThr: 3.966 ± 1.372
4.271LeuVal: 4.271 ± 0.988
0.915LeuTrp: 0.915 ± 0.534
4.271LeuTyr: 4.271 ± 0.957
0.0LeuXaa: 0.0 ± 0.0
Met
3.051MetAla: 3.051 ± 0.887
0.61MetCys: 0.61 ± 0.399
0.915MetAsp: 0.915 ± 0.586
1.22MetGlu: 1.22 ± 0.615
0.61MetPhe: 0.61 ± 0.445
0.61MetGly: 0.61 ± 0.349
0.0MetHis: 0.0 ± 0.0
2.135MetIle: 2.135 ± 0.743
2.746MetLys: 2.746 ± 1.041
1.83MetLeu: 1.83 ± 0.81
0.915MetMet: 0.915 ± 0.606
3.051MetAsn: 3.051 ± 0.84
0.0MetPro: 0.0 ± 0.0
0.305MetGln: 0.305 ± 0.363
1.22MetArg: 1.22 ± 0.618
1.22MetSer: 1.22 ± 0.601
0.61MetThr: 0.61 ± 0.535
0.915MetVal: 0.915 ± 0.565
0.0MetTrp: 0.0 ± 0.0
0.915MetTyr: 0.915 ± 0.488
0.0MetXaa: 0.0 ± 0.0
Asn
3.051AsnAla: 3.051 ± 1.055
0.0AsnCys: 0.0 ± 0.0
5.491AsnAsp: 5.491 ± 1.031
5.796AsnGlu: 5.796 ± 1.618
1.83AsnPhe: 1.83 ± 0.75
3.661AsnGly: 3.661 ± 1.172
1.83AsnHis: 1.83 ± 0.564
6.711AsnIle: 6.711 ± 1.998
6.406AsnLys: 6.406 ± 1.316
3.661AsnLeu: 3.661 ± 1.118
1.525AsnMet: 1.525 ± 0.582
6.406AsnAsn: 6.406 ± 1.586
2.135AsnPro: 2.135 ± 0.852
2.135AsnGln: 2.135 ± 0.822
2.441AsnArg: 2.441 ± 0.967
3.051AsnSer: 3.051 ± 0.933
3.966AsnThr: 3.966 ± 0.967
5.796AsnVal: 5.796 ± 1.427
0.0AsnTrp: 0.0 ± 0.0
3.356AsnTyr: 3.356 ± 0.827
0.0AsnXaa: 0.0 ± 0.0
Pro
0.915ProAla: 0.915 ± 0.516
0.305ProCys: 0.305 ± 0.302
1.22ProAsp: 1.22 ± 0.679
0.915ProGlu: 0.915 ± 0.714
0.61ProPhe: 0.61 ± 0.391
0.61ProGly: 0.61 ± 0.51
0.61ProHis: 0.61 ± 0.382
4.271ProIle: 4.271 ± 1.163
1.83ProLys: 1.83 ± 0.764
1.22ProLeu: 1.22 ± 0.434
0.305ProMet: 0.305 ± 0.379
2.746ProAsn: 2.746 ± 0.672
0.61ProPro: 0.61 ± 0.439
0.915ProGln: 0.915 ± 0.412
0.305ProArg: 0.305 ± 0.255
1.83ProSer: 1.83 ± 0.542
0.915ProThr: 0.915 ± 0.518
0.915ProVal: 0.915 ± 0.502
0.0ProTrp: 0.0 ± 0.0
0.915ProTyr: 0.915 ± 0.445
0.0ProXaa: 0.0 ± 0.0
Gln
2.135GlnAla: 2.135 ± 0.672
0.61GlnCys: 0.61 ± 0.361
1.525GlnAsp: 1.525 ± 0.549
1.83GlnGlu: 1.83 ± 0.628
2.746GlnPhe: 2.746 ± 0.751
1.22GlnGly: 1.22 ± 0.747
1.22GlnHis: 1.22 ± 0.599
1.525GlnIle: 1.525 ± 0.706
2.746GlnLys: 2.746 ± 0.755
3.966GlnLeu: 3.966 ± 0.933
0.915GlnMet: 0.915 ± 0.498
1.83GlnAsn: 1.83 ± 0.528
1.22GlnPro: 1.22 ± 0.582
0.61GlnGln: 0.61 ± 0.51
1.525GlnArg: 1.525 ± 0.721
4.881GlnSer: 4.881 ± 0.944
1.22GlnThr: 1.22 ± 0.508
3.051GlnVal: 3.051 ± 1.129
0.305GlnTrp: 0.305 ± 0.278
1.525GlnTyr: 1.525 ± 0.683
0.0GlnXaa: 0.0 ± 0.0
Arg
1.22ArgAla: 1.22 ± 0.63
0.305ArgCys: 0.305 ± 0.278
2.746ArgAsp: 2.746 ± 0.934
2.441ArgGlu: 2.441 ± 1.026
3.051ArgPhe: 3.051 ± 0.928
1.525ArgGly: 1.525 ± 0.51
1.525ArgHis: 1.525 ± 0.728
3.051ArgIle: 3.051 ± 0.859
2.746ArgLys: 2.746 ± 0.693
3.356ArgLeu: 3.356 ± 0.925
0.0ArgMet: 0.0 ± 0.0
2.746ArgAsn: 2.746 ± 0.813
0.61ArgPro: 0.61 ± 0.335
2.441ArgGln: 2.441 ± 0.639
1.83ArgArg: 1.83 ± 0.806
3.356ArgSer: 3.356 ± 1.132
1.83ArgThr: 1.83 ± 0.777
2.135ArgVal: 2.135 ± 0.726
0.305ArgTrp: 0.305 ± 0.305
1.83ArgTyr: 1.83 ± 0.566
0.0ArgXaa: 0.0 ± 0.0
Ser
4.271SerAla: 4.271 ± 1.377
0.0SerCys: 0.0 ± 0.0
4.271SerAsp: 4.271 ± 0.954
5.491SerGlu: 5.491 ± 1.582
2.441SerPhe: 2.441 ± 0.735
2.135SerGly: 2.135 ± 1.088
0.61SerHis: 0.61 ± 0.352
4.881SerIle: 4.881 ± 0.988
9.762SerLys: 9.762 ± 1.401
3.661SerLeu: 3.661 ± 1.052
1.83SerMet: 1.83 ± 0.738
4.881SerAsn: 4.881 ± 1.34
2.135SerPro: 2.135 ± 0.511
1.22SerGln: 1.22 ± 0.541
2.135SerArg: 2.135 ± 0.927
3.051SerSer: 3.051 ± 0.981
3.661SerThr: 3.661 ± 1.189
3.356SerVal: 3.356 ± 1.127
0.61SerTrp: 0.61 ± 0.51
3.661SerTyr: 3.661 ± 0.915
0.0SerXaa: 0.0 ± 0.0
Thr
1.83ThrAla: 1.83 ± 0.776
0.61ThrCys: 0.61 ± 0.399
4.576ThrAsp: 4.576 ± 1.506
3.966ThrGlu: 3.966 ± 1.12
1.525ThrPhe: 1.525 ± 0.489
3.661ThrGly: 3.661 ± 0.788
1.83ThrHis: 1.83 ± 0.686
3.966ThrIle: 3.966 ± 1.252
4.576ThrLys: 4.576 ± 1.059
4.271ThrLeu: 4.271 ± 0.858
0.61ThrMet: 0.61 ± 0.418
3.051ThrAsn: 3.051 ± 0.833
1.22ThrPro: 1.22 ± 0.565
3.051ThrGln: 3.051 ± 0.914
1.525ThrArg: 1.525 ± 0.621
2.441ThrSer: 2.441 ± 0.902
4.576ThrThr: 4.576 ± 1.306
2.746ThrVal: 2.746 ± 1.198
0.915ThrTrp: 0.915 ± 0.612
2.135ThrTyr: 2.135 ± 0.899
0.0ThrXaa: 0.0 ± 0.0
Val
3.356ValAla: 3.356 ± 0.919
1.22ValCys: 1.22 ± 0.549
4.881ValAsp: 4.881 ± 0.889
3.051ValGlu: 3.051 ± 0.792
2.746ValPhe: 2.746 ± 1.038
3.966ValGly: 3.966 ± 1.163
0.915ValHis: 0.915 ± 0.511
6.101ValIle: 6.101 ± 1.009
4.271ValLys: 4.271 ± 1.332
4.881ValLeu: 4.881 ± 1.421
1.83ValMet: 1.83 ± 0.621
4.881ValAsn: 4.881 ± 1.308
1.22ValPro: 1.22 ± 0.757
2.746ValGln: 2.746 ± 0.907
1.83ValArg: 1.83 ± 0.747
3.966ValSer: 3.966 ± 1.016
3.356ValThr: 3.356 ± 0.96
4.271ValVal: 4.271 ± 1.304
0.61ValTrp: 0.61 ± 0.378
2.746ValTyr: 2.746 ± 1.007
0.0ValXaa: 0.0 ± 0.0
Trp
0.915TrpAla: 0.915 ± 0.508
0.0TrpCys: 0.0 ± 0.0
0.61TrpAsp: 0.61 ± 0.389
0.0TrpGlu: 0.0 ± 0.0
0.61TrpPhe: 0.61 ± 0.428
0.0TrpGly: 0.0 ± 0.0
0.305TrpHis: 0.305 ± 0.278
0.915TrpIle: 0.915 ± 0.478
1.525TrpLys: 1.525 ± 0.719
0.915TrpLeu: 0.915 ± 0.497
0.61TrpMet: 0.61 ± 0.358
0.305TrpAsn: 0.305 ± 0.352
0.0TrpPro: 0.0 ± 0.0
0.0TrpGln: 0.0 ± 0.0
1.22TrpArg: 1.22 ± 0.55
0.915TrpSer: 0.915 ± 0.597
0.0TrpThr: 0.0 ± 0.0
0.0TrpVal: 0.0 ± 0.0
0.0TrpTrp: 0.0 ± 0.0
1.525TrpTyr: 1.525 ± 0.636
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.83TyrAla: 1.83 ± 0.96
0.305TyrCys: 0.305 ± 0.255
2.135TyrAsp: 2.135 ± 0.741
3.051TyrGlu: 3.051 ± 1.072
1.22TyrPhe: 1.22 ± 0.572
3.051TyrGly: 3.051 ± 1.062
2.135TyrHis: 2.135 ± 0.981
5.186TyrIle: 5.186 ± 0.997
5.491TyrLys: 5.491 ± 1.082
2.135TyrLeu: 2.135 ± 0.729
1.22TyrMet: 1.22 ± 0.637
3.661TyrAsn: 3.661 ± 0.828
0.915TyrPro: 0.915 ± 0.481
3.051TyrGln: 3.051 ± 0.851
2.746TyrArg: 2.746 ± 0.805
3.356TyrSer: 3.356 ± 0.849
1.525TyrThr: 1.525 ± 0.532
2.441TyrVal: 2.441 ± 0.716
1.22TyrTrp: 1.22 ± 0.78
3.051TyrTyr: 3.051 ± 0.745
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 20 proteins (3279 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski