Malaciobacter mytili LMG 24559
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2879 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345J5I4|A0A345J5I4_9PROT Phosphoribosylamine--glycine ligase OS=Malaciobacter mytili LMG 24559 OX=1032238 GN=purD PE=3 SV=1
MM1 pKa = 7.48 TKK3 pKa = 10.21 QISKK7 pKa = 10.74 NILFNTFKK15 pKa = 11.29 VEE17 pKa = 4.32 DD18 pKa = 3.93 FTSLEE23 pKa = 3.95 EE24 pKa = 4.61 VINNMPPSVVEE35 pKa = 4.02 YY36 pKa = 11.07 YY37 pKa = 10.96 LDD39 pKa = 3.84 NLDD42 pKa = 4.08 QIEE45 pKa = 4.45 DD46 pKa = 3.28 NSYY49 pKa = 10.91 LNKK52 pKa = 9.94 RR53 pKa = 11.84 DD54 pKa = 3.91 IEE56 pKa = 4.19 HH57 pKa = 6.57 SLYY60 pKa = 10.43 FGNYY64 pKa = 8.75 SLYY67 pKa = 10.86 LDD69 pKa = 4.09 YY70 pKa = 11.37 SDD72 pKa = 5.93 NIYY75 pKa = 11.11 LEE77 pKa = 4.34 IDD79 pKa = 3.31 IKK81 pKa = 11.34 DD82 pKa = 3.78 EE83 pKa = 4.1 EE84 pKa = 4.64 TDD86 pKa = 3.86 SFWW89 pKa = 5.47
Molecular weight: 10.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.757
IPC2_protein 3.935
IPC_protein 3.884
Toseland 3.681
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.783
Rodwell 3.706
Grimsley 3.592
Solomon 3.846
Lehninger 3.795
Nozaki 3.986
DTASelect 4.177
Thurlkill 3.732
EMBOSS 3.795
Sillero 3.999
Patrickios 0.896
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.879
Protein with the highest isoelectric point:
>tr|A0A345J753|A0A345J753_9PROT Ribonuclease HII OS=Malaciobacter mytili LMG 24559 OX=1032238 GN=rnhB PE=3 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.04 QPHH8 pKa = 4.86 NTPRR12 pKa = 11.84 KK13 pKa = 7.31 RR14 pKa = 11.84 THH16 pKa = 5.83 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MATKK25 pKa = 10.25 NGRR28 pKa = 11.84 KK29 pKa = 9.01 IIKK32 pKa = 9.57 ARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.81 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.414
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.384
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2879
0
2879
928648
37
10784
322.6
36.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.708 ± 0.052
0.908 ± 0.021
5.391 ± 0.096
7.375 ± 0.065
5.343 ± 0.056
5.197 ± 0.112
1.458 ± 0.021
9.873 ± 0.049
9.867 ± 0.104
9.847 ± 0.069
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.116 ± 0.025
6.724 ± 0.063
2.536 ± 0.034
2.864 ± 0.029
2.692 ± 0.035
6.49 ± 0.054
5.137 ± 0.069
5.604 ± 0.045
0.675 ± 0.013
4.195 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here