Streptomyces phyllanthi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8637 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5N8W7H7|A0A5N8W7H7_9ACTN Sugar porter family MFS transporter OS=Streptomyces phyllanthi OX=1803180 GN=FNH04_27025 PE=3 SV=1
MM1 pKa = 6.89TQPPGQPPQDD11 pKa = 3.66GYY13 pKa = 11.59GAQPPQQPPYY23 pKa = 8.79PGPYY27 pKa = 9.45SPPPQPPTQVPGQSAQPGQPGQPPNPYY54 pKa = 9.97AAPQPQGPYY63 pKa = 10.05GAPQQPYY70 pKa = 9.8GAPQPPYY77 pKa = 10.16AGAPGAPGTPGMPGMPNYY95 pKa = 8.83GAPYY99 pKa = 9.64GGYY102 pKa = 9.26PPPPPPGGGGSKK114 pKa = 10.49NRR116 pKa = 11.84MALIIVGAVTAVAVVVGGIAVATSGSDD143 pKa = 3.3EE144 pKa = 4.44SSGATSDD151 pKa = 3.84KK152 pKa = 10.87NVSAEE157 pKa = 3.86PSVSDD162 pKa = 3.94GASEE166 pKa = 3.99EE167 pKa = 4.37AADD170 pKa = 4.9DD171 pKa = 4.37PSTEE175 pKa = 4.11ATDD178 pKa = 4.23DD179 pKa = 4.33PSDD182 pKa = 3.55SGYY185 pKa = 11.2SDD187 pKa = 3.55DD188 pKa = 4.02TGGGSEE194 pKa = 4.38EE195 pKa = 4.48APPEE199 pKa = 4.04PSVEE203 pKa = 4.29GQWQDD208 pKa = 3.55ADD210 pKa = 3.86AKK212 pKa = 9.52TLTVGTKK219 pKa = 8.33YY220 pKa = 9.53TSGDD224 pKa = 3.44LKK226 pKa = 11.03GKK228 pKa = 10.0YY229 pKa = 9.29ALSYY233 pKa = 10.72IDD235 pKa = 3.55TTGKK239 pKa = 10.87GILTGLGAYY248 pKa = 10.0RR249 pKa = 11.84DD250 pKa = 4.01DD251 pKa = 4.41DD252 pKa = 3.92SFRR255 pKa = 11.84LVLRR259 pKa = 11.84PMDD262 pKa = 4.45SDD264 pKa = 4.11SKK266 pKa = 11.55DD267 pKa = 3.4EE268 pKa = 3.99SDD270 pKa = 3.73YY271 pKa = 11.57VYY273 pKa = 10.29GTVRR277 pKa = 11.84RR278 pKa = 11.84SGDD281 pKa = 3.43DD282 pKa = 4.18VVITWDD288 pKa = 3.75DD289 pKa = 3.55GGKK292 pKa = 8.15EE293 pKa = 3.87TLGYY297 pKa = 10.05IGSLSDD303 pKa = 3.22

Molecular weight: 30.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5N8VWB1|A0A5N8VWB1_9ACTN Transposase family protein OS=Streptomyces phyllanthi OX=1803180 GN=FNH04_03235 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.75GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8637

0

8637

2865135

20

3672

331.7

35.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.011 ± 0.04

0.799 ± 0.007

6.063 ± 0.02

5.855 ± 0.025

2.759 ± 0.015

9.462 ± 0.028

2.313 ± 0.012

3.165 ± 0.017

2.191 ± 0.024

10.202 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.72 ± 0.01

1.861 ± 0.016

6.028 ± 0.02

2.746 ± 0.016

8.088 ± 0.032

5.258 ± 0.02

6.279 ± 0.025

8.484 ± 0.026

1.552 ± 0.011

2.164 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski