Pseudomonas phage PlaquesPlease

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Ghunavirus; unclassified Ghunavirus

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G8LJS0|A0A7G8LJS0_9CAUD Glycosyltransferase OS=Pseudomonas phage PlaquesPlease OX=2762289 PE=4 SV=1
MM1 pKa = 7.52SVEE4 pKa = 4.24TLRR7 pKa = 11.84LIPSSRR13 pKa = 11.84LLEE16 pKa = 4.05LLQKK20 pKa = 9.72EE21 pKa = 4.28EE22 pKa = 3.85RR23 pKa = 11.84LYY25 pKa = 11.04ALEE28 pKa = 4.42SGGVDD33 pKa = 2.59SWEE36 pKa = 4.85GYY38 pKa = 10.91DD39 pKa = 4.17DD40 pKa = 3.99ALEE43 pKa = 3.9IHH45 pKa = 6.75EE46 pKa = 5.17DD47 pKa = 3.11QWEE50 pKa = 4.01ADD52 pKa = 3.8QLLEE56 pKa = 4.31TYY58 pKa = 10.3PEE60 pKa = 3.95STYY63 pKa = 10.22QQ64 pKa = 3.41

Molecular weight: 7.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G8LJS2|A0A7G8LJS2_9CAUD Uncharacterized protein OS=Pseudomonas phage PlaquesPlease OX=2762289 PE=4 SV=1
MM1 pKa = 7.12MHH3 pKa = 7.03TNYY6 pKa = 10.04QPARR10 pKa = 11.84KK11 pKa = 9.06GLYY14 pKa = 8.86EE15 pKa = 4.12SQIHH19 pKa = 6.1AEE21 pKa = 4.05KK22 pKa = 10.57EE23 pKa = 3.64LRR25 pKa = 11.84TRR27 pKa = 11.84KK28 pKa = 9.75NLGRR32 pKa = 11.84PGQAGEE38 pKa = 4.16LLGVTLAGFQRR49 pKa = 11.84KK50 pKa = 7.82RR51 pKa = 11.84LRR53 pKa = 11.84YY54 pKa = 9.1SDD56 pKa = 4.56RR57 pKa = 11.84GDD59 pKa = 3.19FTRR62 pKa = 11.84CYY64 pKa = 10.34RR65 pKa = 11.84GGFF68 pKa = 3.49

Molecular weight: 7.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

12846

45

1393

256.9

28.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.7 ± 0.418

0.965 ± 0.158

6.399 ± 0.27

6.796 ± 0.379

3.752 ± 0.209

7.909 ± 0.305

2.001 ± 0.191

5.223 ± 0.243

5.667 ± 0.309

7.995 ± 0.339

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.608 ± 0.162

3.986 ± 0.226

4.134 ± 0.212

4.227 ± 0.326

6.111 ± 0.237

5.846 ± 0.255

5.364 ± 0.249

6.648 ± 0.332

1.479 ± 0.141

3.192 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski