Amino acid dipepetide frequency for Pseudomonas phage PlaquesPlease

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.588AlaAla: 10.588 ± 1.561
1.168AlaCys: 1.168 ± 0.318
5.839AlaAsp: 5.839 ± 0.772
6.384AlaGlu: 6.384 ± 0.909
2.958AlaPhe: 2.958 ± 0.636
6.929AlaGly: 6.929 ± 0.885
1.246AlaHis: 1.246 ± 0.255
5.761AlaIle: 5.761 ± 0.629
5.761AlaLys: 5.761 ± 0.722
8.408AlaLeu: 8.408 ± 0.811
3.192AlaMet: 3.192 ± 0.741
4.126AlaAsn: 4.126 ± 0.588
2.88AlaPro: 2.88 ± 0.466
4.593AlaGln: 4.593 ± 0.623
6.462AlaArg: 6.462 ± 0.725
5.216AlaSer: 5.216 ± 0.693
5.06AlaThr: 5.06 ± 0.745
5.527AlaVal: 5.527 ± 0.788
1.401AlaTrp: 1.401 ± 0.346
3.503AlaTyr: 3.503 ± 0.535
0.0AlaXaa: 0.0 ± 0.0
Cys
1.323CysAla: 1.323 ± 0.395
0.156CysCys: 0.156 ± 0.101
0.545CysAsp: 0.545 ± 0.256
0.467CysGlu: 0.467 ± 0.176
0.623CysPhe: 0.623 ± 0.215
0.701CysGly: 0.701 ± 0.21
0.234CysHis: 0.234 ± 0.124
0.389CysIle: 0.389 ± 0.163
0.856CysLys: 0.856 ± 0.292
0.623CysLeu: 0.623 ± 0.263
0.234CysMet: 0.234 ± 0.12
0.234CysAsn: 0.234 ± 0.133
0.467CysPro: 0.467 ± 0.178
0.389CysGln: 0.389 ± 0.166
0.623CysArg: 0.623 ± 0.274
0.311CysSer: 0.311 ± 0.239
0.234CysThr: 0.234 ± 0.134
0.856CysVal: 0.856 ± 0.279
0.156CysTrp: 0.156 ± 0.125
0.234CysTyr: 0.234 ± 0.133
0.0CysXaa: 0.0 ± 0.0
Asp
5.45AspAla: 5.45 ± 0.575
0.545AspCys: 0.545 ± 0.239
4.438AspAsp: 4.438 ± 0.811
4.126AspGlu: 4.126 ± 0.67
3.815AspPhe: 3.815 ± 0.47
5.527AspGly: 5.527 ± 0.494
1.401AspHis: 1.401 ± 0.405
3.581AspIle: 3.581 ± 0.651
3.581AspLys: 3.581 ± 0.629
5.294AspLeu: 5.294 ± 0.587
2.18AspMet: 2.18 ± 0.406
2.491AspAsn: 2.491 ± 0.382
3.581AspPro: 3.581 ± 0.831
1.713AspGln: 1.713 ± 0.351
3.192AspArg: 3.192 ± 0.568
3.27AspSer: 3.27 ± 0.533
2.958AspThr: 2.958 ± 0.504
3.581AspVal: 3.581 ± 0.595
1.168AspTrp: 1.168 ± 0.279
2.024AspTyr: 2.024 ± 0.32
0.0AspXaa: 0.0 ± 0.0
Glu
8.097GluAla: 8.097 ± 0.982
0.701GluCys: 0.701 ± 0.261
3.737GluAsp: 3.737 ± 0.361
4.982GluGlu: 4.982 ± 0.883
2.88GluPhe: 2.88 ± 0.36
5.294GluGly: 5.294 ± 0.731
1.246GluHis: 1.246 ± 0.352
2.803GluIle: 2.803 ± 0.431
2.647GluLys: 2.647 ± 0.528
5.216GluLeu: 5.216 ± 0.832
1.557GluMet: 1.557 ± 0.346
2.258GluAsn: 2.258 ± 0.431
1.713GluPro: 1.713 ± 0.344
3.97GluGln: 3.97 ± 0.709
4.671GluArg: 4.671 ± 0.56
3.659GluSer: 3.659 ± 0.456
3.581GluThr: 3.581 ± 0.54
5.06GluVal: 5.06 ± 0.772
1.323GluTrp: 1.323 ± 0.5
2.569GluTyr: 2.569 ± 0.488
0.0GluXaa: 0.0 ± 0.0
Phe
3.348PheAla: 3.348 ± 0.416
0.623PheCys: 0.623 ± 0.231
2.958PheAsp: 2.958 ± 0.496
1.635PheGlu: 1.635 ± 0.277
1.479PhePhe: 1.479 ± 0.297
3.036PheGly: 3.036 ± 0.448
0.856PheHis: 0.856 ± 0.259
2.18PheIle: 2.18 ± 0.501
1.713PheLys: 1.713 ± 0.393
2.647PheLeu: 2.647 ± 0.458
1.09PheMet: 1.09 ± 0.334
1.868PheAsn: 1.868 ± 0.382
1.713PhePro: 1.713 ± 0.366
1.323PheGln: 1.323 ± 0.416
2.258PheArg: 2.258 ± 0.42
2.258PheSer: 2.258 ± 0.481
2.803PheThr: 2.803 ± 0.444
2.647PheVal: 2.647 ± 0.41
0.545PheTrp: 0.545 ± 0.217
0.545PheTyr: 0.545 ± 0.189
0.0PheXaa: 0.0 ± 0.0
Gly
6.929GlyAla: 6.929 ± 1.21
0.856GlyCys: 0.856 ± 0.235
5.527GlyAsp: 5.527 ± 0.654
4.749GlyGlu: 4.749 ± 0.611
2.88GlyPhe: 2.88 ± 0.494
6.773GlyGly: 6.773 ± 0.917
2.024GlyHis: 2.024 ± 0.5
4.749GlyIle: 4.749 ± 0.658
4.282GlyLys: 4.282 ± 0.605
6.306GlyLeu: 6.306 ± 1.127
1.713GlyMet: 1.713 ± 0.353
2.413GlyAsn: 2.413 ± 0.47
2.569GlyPro: 2.569 ± 0.458
3.348GlyGln: 3.348 ± 0.514
4.515GlyArg: 4.515 ± 0.502
5.527GlySer: 5.527 ± 0.614
4.515GlyThr: 4.515 ± 0.527
4.515GlyVal: 4.515 ± 0.622
1.791GlyTrp: 1.791 ± 0.351
3.114GlyTyr: 3.114 ± 0.492
0.0GlyXaa: 0.0 ± 0.0
His
1.012HisAla: 1.012 ± 0.339
0.467HisCys: 0.467 ± 0.223
1.246HisAsp: 1.246 ± 0.269
1.401HisGlu: 1.401 ± 0.407
0.779HisPhe: 0.779 ± 0.263
1.791HisGly: 1.791 ± 0.347
0.311HisHis: 0.311 ± 0.187
1.557HisIle: 1.557 ± 0.358
1.168HisLys: 1.168 ± 0.276
1.713HisLeu: 1.713 ± 0.319
0.934HisMet: 0.934 ± 0.269
0.701HisAsn: 0.701 ± 0.214
0.779HisPro: 0.779 ± 0.28
0.701HisGln: 0.701 ± 0.251
0.856HisArg: 0.856 ± 0.296
0.856HisSer: 0.856 ± 0.318
0.934HisThr: 0.934 ± 0.258
1.557HisVal: 1.557 ± 0.42
0.545HisTrp: 0.545 ± 0.273
0.701HisTyr: 0.701 ± 0.207
0.0HisXaa: 0.0 ± 0.0
Ile
4.827IleAla: 4.827 ± 0.655
0.623IleCys: 0.623 ± 0.211
3.425IleAsp: 3.425 ± 0.516
3.659IleGlu: 3.659 ± 0.583
1.09IlePhe: 1.09 ± 0.369
3.348IleGly: 3.348 ± 0.484
1.323IleHis: 1.323 ± 0.371
2.647IleIle: 2.647 ± 0.544
3.97IleLys: 3.97 ± 0.487
3.97IleLeu: 3.97 ± 0.42
0.856IleMet: 0.856 ± 0.281
2.491IleAsn: 2.491 ± 0.539
2.413IlePro: 2.413 ± 0.561
1.635IleGln: 1.635 ± 0.356
3.581IleArg: 3.581 ± 0.524
2.413IleSer: 2.413 ± 0.493
3.659IleThr: 3.659 ± 0.515
3.893IleVal: 3.893 ± 0.542
0.467IleTrp: 0.467 ± 0.206
1.946IleTyr: 1.946 ± 0.422
0.0IleXaa: 0.0 ± 0.0
Lys
5.917LysAla: 5.917 ± 0.83
0.467LysCys: 0.467 ± 0.176
4.048LysAsp: 4.048 ± 0.567
3.815LysGlu: 3.815 ± 0.449
2.413LysPhe: 2.413 ± 0.377
4.749LysGly: 4.749 ± 0.677
1.323LysHis: 1.323 ± 0.404
2.102LysIle: 2.102 ± 0.463
2.958LysLys: 2.958 ± 0.584
5.138LysLeu: 5.138 ± 0.79
1.09LysMet: 1.09 ± 0.302
1.868LysAsn: 1.868 ± 0.342
2.725LysPro: 2.725 ± 0.595
2.413LysGln: 2.413 ± 0.422
3.425LysArg: 3.425 ± 0.577
2.413LysSer: 2.413 ± 0.476
2.647LysThr: 2.647 ± 0.549
4.905LysVal: 4.905 ± 0.57
0.545LysTrp: 0.545 ± 0.276
1.713LysTyr: 1.713 ± 0.277
0.0LysXaa: 0.0 ± 0.0
Leu
8.097LeuAla: 8.097 ± 0.847
0.389LeuCys: 0.389 ± 0.171
4.515LeuAsp: 4.515 ± 0.525
5.605LeuGlu: 5.605 ± 0.693
2.491LeuPhe: 2.491 ± 0.512
5.605LeuGly: 5.605 ± 0.624
1.946LeuHis: 1.946 ± 0.304
4.126LeuIle: 4.126 ± 0.57
6.306LeuLys: 6.306 ± 0.683
4.827LeuLeu: 4.827 ± 0.663
2.725LeuMet: 2.725 ± 0.443
3.737LeuAsn: 3.737 ± 0.843
2.725LeuPro: 2.725 ± 0.394
3.97LeuGln: 3.97 ± 0.651
5.683LeuArg: 5.683 ± 0.642
4.827LeuSer: 4.827 ± 0.661
4.048LeuThr: 4.048 ± 0.664
5.45LeuVal: 5.45 ± 0.807
0.856LeuTrp: 0.856 ± 0.256
2.024LeuTyr: 2.024 ± 0.445
0.0LeuXaa: 0.0 ± 0.0
Met
2.803MetAla: 2.803 ± 0.473
0.156MetCys: 0.156 ± 0.119
1.635MetAsp: 1.635 ± 0.348
2.336MetGlu: 2.336 ± 0.541
0.701MetPhe: 0.701 ± 0.258
2.024MetGly: 2.024 ± 0.348
0.467MetHis: 0.467 ± 0.199
1.713MetIle: 1.713 ± 0.353
1.168MetLys: 1.168 ± 0.335
2.569MetLeu: 2.569 ± 0.387
0.311MetMet: 0.311 ± 0.145
1.323MetAsn: 1.323 ± 0.37
1.479MetPro: 1.479 ± 0.328
0.623MetGln: 0.623 ± 0.223
0.779MetArg: 0.779 ± 0.217
2.18MetSer: 2.18 ± 0.365
2.336MetThr: 2.336 ± 0.465
1.168MetVal: 1.168 ± 0.31
0.234MetTrp: 0.234 ± 0.121
0.078MetTyr: 0.078 ± 0.075
0.0MetXaa: 0.0 ± 0.0
Asn
3.893AsnAla: 3.893 ± 0.486
0.467AsnCys: 0.467 ± 0.201
2.647AsnAsp: 2.647 ± 0.533
2.88AsnGlu: 2.88 ± 0.607
1.246AsnPhe: 1.246 ± 0.371
4.749AsnGly: 4.749 ± 0.789
0.389AsnHis: 0.389 ± 0.215
1.479AsnIle: 1.479 ± 0.401
0.623AsnLys: 0.623 ± 0.29
2.647AsnLeu: 2.647 ± 0.418
1.401AsnMet: 1.401 ± 0.444
1.713AsnAsn: 1.713 ± 0.359
2.958AsnPro: 2.958 ± 0.359
1.635AsnGln: 1.635 ± 0.308
2.18AsnArg: 2.18 ± 0.551
2.647AsnSer: 2.647 ± 0.534
1.868AsnThr: 1.868 ± 0.344
2.413AsnVal: 2.413 ± 0.518
0.701AsnTrp: 0.701 ± 0.223
1.323AsnTyr: 1.323 ± 0.338
0.0AsnXaa: 0.0 ± 0.0
Pro
3.893ProAla: 3.893 ± 0.463
0.311ProCys: 0.311 ± 0.162
3.425ProAsp: 3.425 ± 0.509
4.204ProGlu: 4.204 ± 0.477
1.557ProPhe: 1.557 ± 0.348
1.635ProGly: 1.635 ± 0.342
1.323ProHis: 1.323 ± 0.406
1.479ProIle: 1.479 ± 0.329
2.491ProLys: 2.491 ± 0.508
2.725ProLeu: 2.725 ± 0.447
0.934ProMet: 0.934 ± 0.226
2.18ProAsn: 2.18 ± 0.525
1.868ProPro: 1.868 ± 0.588
1.479ProGln: 1.479 ± 0.295
2.18ProArg: 2.18 ± 0.375
2.725ProSer: 2.725 ± 0.425
2.18ProThr: 2.18 ± 0.471
3.425ProVal: 3.425 ± 0.405
0.389ProTrp: 0.389 ± 0.269
0.934ProTyr: 0.934 ± 0.26
0.0ProXaa: 0.0 ± 0.0
Gln
5.917GlnAla: 5.917 ± 0.831
0.234GlnCys: 0.234 ± 0.149
2.413GlnAsp: 2.413 ± 0.458
3.27GlnGlu: 3.27 ± 0.668
1.791GlnPhe: 1.791 ± 0.341
3.425GlnGly: 3.425 ± 0.622
0.623GlnHis: 0.623 ± 0.307
1.791GlnIle: 1.791 ± 0.425
1.868GlnLys: 1.868 ± 0.551
3.815GlnLeu: 3.815 ± 0.583
0.934GlnMet: 0.934 ± 0.288
1.168GlnAsn: 1.168 ± 0.314
1.323GlnPro: 1.323 ± 0.261
3.114GlnGln: 3.114 ± 0.644
3.114GlnArg: 3.114 ± 0.604
1.946GlnSer: 1.946 ± 0.537
1.09GlnThr: 1.09 ± 0.295
2.258GlnVal: 2.258 ± 0.342
0.701GlnTrp: 0.701 ± 0.287
1.479GlnTyr: 1.479 ± 0.264
0.0GlnXaa: 0.0 ± 0.0
Arg
5.605ArgAla: 5.605 ± 0.572
0.467ArgCys: 0.467 ± 0.211
3.425ArgAsp: 3.425 ± 0.378
4.671ArgGlu: 4.671 ± 0.687
2.413ArgPhe: 2.413 ± 0.529
4.982ArgGly: 4.982 ± 0.666
1.168ArgHis: 1.168 ± 0.379
3.425ArgIle: 3.425 ± 0.52
3.893ArgLys: 3.893 ± 0.598
5.761ArgLeu: 5.761 ± 0.625
1.479ArgMet: 1.479 ± 0.293
2.803ArgAsn: 2.803 ± 0.473
2.024ArgPro: 2.024 ± 0.408
2.258ArgGln: 2.258 ± 0.522
3.659ArgArg: 3.659 ± 0.477
3.737ArgSer: 3.737 ± 0.644
2.413ArgThr: 2.413 ± 0.44
3.737ArgVal: 3.737 ± 0.56
1.012ArgTrp: 1.012 ± 0.261
2.18ArgTyr: 2.18 ± 0.357
0.0ArgXaa: 0.0 ± 0.0
Ser
5.45SerAla: 5.45 ± 0.697
0.467SerCys: 0.467 ± 0.19
4.515SerAsp: 4.515 ± 0.571
2.803SerGlu: 2.803 ± 0.403
2.803SerPhe: 2.803 ± 0.57
5.372SerGly: 5.372 ± 0.862
0.856SerHis: 0.856 ± 0.219
3.425SerIle: 3.425 ± 0.587
2.958SerLys: 2.958 ± 0.557
4.282SerLeu: 4.282 ± 0.636
1.168SerMet: 1.168 ± 0.322
1.946SerAsn: 1.946 ± 0.377
2.413SerPro: 2.413 ± 0.457
2.413SerGln: 2.413 ± 0.529
3.503SerArg: 3.503 ± 0.631
4.982SerSer: 4.982 ± 0.946
2.803SerThr: 2.803 ± 0.456
3.659SerVal: 3.659 ± 0.57
0.701SerTrp: 0.701 ± 0.219
1.946SerTyr: 1.946 ± 0.458
0.0SerXaa: 0.0 ± 0.0
Thr
3.97ThrAla: 3.97 ± 0.61
0.389ThrCys: 0.389 ± 0.192
3.737ThrAsp: 3.737 ± 0.555
2.88ThrGlu: 2.88 ± 0.492
2.102ThrPhe: 2.102 ± 0.471
4.204ThrGly: 4.204 ± 0.534
0.701ThrHis: 0.701 ± 0.263
4.204ThrIle: 4.204 ± 0.689
4.438ThrLys: 4.438 ± 0.437
3.581ThrLeu: 3.581 ± 0.529
1.09ThrMet: 1.09 ± 0.227
2.336ThrAsn: 2.336 ± 0.529
2.413ThrPro: 2.413 ± 0.316
1.868ThrGln: 1.868 ± 0.422
3.114ThrArg: 3.114 ± 0.506
3.036ThrSer: 3.036 ± 0.552
3.114ThrThr: 3.114 ± 0.666
4.126ThrVal: 4.126 ± 0.63
0.389ThrTrp: 0.389 ± 0.208
1.946ThrTyr: 1.946 ± 0.358
0.0ThrXaa: 0.0 ± 0.0
Val
5.45ValAla: 5.45 ± 0.65
0.701ValCys: 0.701 ± 0.203
3.192ValAsp: 3.192 ± 0.455
4.827ValGlu: 4.827 ± 0.787
1.635ValPhe: 1.635 ± 0.344
4.671ValGly: 4.671 ± 0.807
1.323ValHis: 1.323 ± 0.424
2.88ValIle: 2.88 ± 0.611
3.348ValLys: 3.348 ± 0.528
5.761ValLeu: 5.761 ± 0.728
1.868ValMet: 1.868 ± 0.409
2.647ValAsn: 2.647 ± 0.496
3.659ValPro: 3.659 ± 0.69
2.336ValGln: 2.336 ± 0.439
4.593ValArg: 4.593 ± 0.527
4.126ValSer: 4.126 ± 0.613
4.671ValThr: 4.671 ± 0.839
5.216ValVal: 5.216 ± 0.761
1.012ValTrp: 1.012 ± 0.254
2.569ValTyr: 2.569 ± 0.543
0.0ValXaa: 0.0 ± 0.0
Trp
1.479TrpAla: 1.479 ± 0.414
0.156TrpCys: 0.156 ± 0.119
0.934TrpAsp: 0.934 ± 0.315
1.012TrpGlu: 1.012 ± 0.354
0.779TrpPhe: 0.779 ± 0.255
1.012TrpGly: 1.012 ± 0.294
0.701TrpHis: 0.701 ± 0.201
0.545TrpIle: 0.545 ± 0.18
0.779TrpLys: 0.779 ± 0.156
1.635TrpLeu: 1.635 ± 0.398
0.389TrpMet: 0.389 ± 0.158
0.389TrpAsn: 0.389 ± 0.174
0.311TrpPro: 0.311 ± 0.164
0.623TrpGln: 0.623 ± 0.296
0.856TrpArg: 0.856 ± 0.213
0.701TrpSer: 0.701 ± 0.269
1.168TrpThr: 1.168 ± 0.281
0.779TrpVal: 0.779 ± 0.251
0.234TrpTrp: 0.234 ± 0.114
0.311TrpTyr: 0.311 ± 0.184
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.958TyrAla: 2.958 ± 0.499
0.311TyrCys: 0.311 ± 0.16
1.791TyrAsp: 1.791 ± 0.444
2.258TyrGlu: 2.258 ± 0.374
1.09TyrPhe: 1.09 ± 0.268
3.27TyrGly: 3.27 ± 0.489
0.545TyrHis: 0.545 ± 0.22
1.401TyrIle: 1.401 ± 0.427
1.868TyrLys: 1.868 ± 0.324
3.036TyrLeu: 3.036 ± 0.438
0.856TyrMet: 0.856 ± 0.275
1.168TyrAsn: 1.168 ± 0.202
1.323TyrPro: 1.323 ± 0.265
1.868TyrGln: 1.868 ± 0.49
1.868TyrArg: 1.868 ± 0.339
1.635TyrSer: 1.635 ± 0.355
1.557TyrThr: 1.557 ± 0.35
1.713TyrVal: 1.713 ± 0.306
0.623TyrTrp: 0.623 ± 0.257
0.779TyrTyr: 0.779 ± 0.256
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (12846 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski