Terribacillus goriensis
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3230 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A075LPV7|A0A075LPV7_9BACI Uncharacterized protein OS=Terribacillus goriensis OX=386490 GN=GZ22_18275 PE=4 SV=1
MM1 pKa = 7.62 KK2 pKa = 10.38 KK3 pKa = 10.43 LFLAFPLLLVFLTACGSQYY22 pKa = 11.06 DD23 pKa = 4.0 PEE25 pKa = 5.01 IEE27 pKa = 4.09 QVIEE31 pKa = 4.34 LEE33 pKa = 4.15 NEE35 pKa = 3.85 HH36 pKa = 6.9 LADD39 pKa = 5.5 LGDD42 pKa = 4.67 DD43 pKa = 3.52 VDD45 pKa = 4.31 LTRR48 pKa = 11.84 DD49 pKa = 3.28 TSDD52 pKa = 3.38 LYY54 pKa = 11.45 VYY56 pKa = 11.18 ADD58 pKa = 3.38 GGYY61 pKa = 9.9 VEE63 pKa = 5.08 IRR65 pKa = 11.84 YY66 pKa = 10.38 DD67 pKa = 3.35 NAAGEE72 pKa = 4.35 EE73 pKa = 4.04 QQAVYY78 pKa = 10.67 EE79 pKa = 4.21 EE80 pKa = 4.87 TGTDD84 pKa = 2.82 TYY86 pKa = 11.05 EE87 pKa = 4.16 RR88 pKa = 11.84 HH89 pKa = 7.06 DD90 pKa = 4.07 GMPEE94 pKa = 3.49 EE95 pKa = 4.94 AEE97 pKa = 4.03 NLTRR101 pKa = 11.84 EE102 pKa = 4.24 YY103 pKa = 11.03 EE104 pKa = 4.14 EE105 pKa = 4.29 EE106 pKa = 3.94 NRR108 pKa = 4.14
Molecular weight: 12.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.897
IPC_protein 3.834
Toseland 3.656
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.694
Rodwell 3.668
Grimsley 3.567
Solomon 3.783
Lehninger 3.745
Nozaki 3.923
DTASelect 4.062
Thurlkill 3.694
EMBOSS 3.706
Sillero 3.948
Patrickios 0.985
IPC_peptide 3.795
IPC2_peptide 3.935
IPC2.peptide.svr19 3.848
Protein with the highest isoelectric point:
>tr|A0A075LKH6|A0A075LKH6_9BACI Shikimate dehydrogenase (NADP(+)) OS=Terribacillus goriensis OX=386490 GN=aroE PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 KK11 pKa = 9.23 RR12 pKa = 11.84 KK13 pKa = 7.99 KK14 pKa = 9.24 VHH16 pKa = 5.91 GFRR19 pKa = 11.84 QRR21 pKa = 11.84 MSTSNGRR28 pKa = 11.84 NVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.05 GRR39 pKa = 11.84 KK40 pKa = 8.7 VLSAA44 pKa = 4.05
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3230
0
3230
951185
30
2839
294.5
32.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.103 ± 0.045
0.602 ± 0.011
5.508 ± 0.04
7.409 ± 0.057
4.338 ± 0.039
6.946 ± 0.037
2.095 ± 0.019
7.427 ± 0.043
6.077 ± 0.042
9.918 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.761 ± 0.022
3.871 ± 0.027
3.627 ± 0.037
4.135 ± 0.036
4.16 ± 0.033
5.889 ± 0.036
5.599 ± 0.034
7.052 ± 0.036
1.008 ± 0.018
3.474 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here