Pseudomonas flexibilis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3275 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B3BXU0|A0A0B3BXU0_9PSED Autotransporter assembly factor TamA OS=Pseudomonas flexibilis OX=706570 GN=PT85_13025 PE=3 SV=1
MM1 pKa = 7.32KK2 pKa = 10.71VMWTLKK8 pKa = 9.98PLAFAVAAGLAFSATAQEE26 pKa = 4.75VPPPTTPGSSAQATVSDD43 pKa = 4.25TQTSTGNYY51 pKa = 9.1VLNQGTVNTASLEE64 pKa = 4.15SSLDD68 pKa = 3.58DD69 pKa = 3.49AAGNVGVNIAGGDD82 pKa = 3.7HH83 pKa = 5.9NQQANSAALSTADD96 pKa = 3.34EE97 pKa = 4.53FFVFGTATASVNLNQTGTNVVDD119 pKa = 4.09NYY121 pKa = 11.35SVTNSASVLDD131 pKa = 4.11SGNNASGNIGINVASGVFNQQKK153 pKa = 10.61NDD155 pKa = 3.31FATATAGGFVATASSTATQSSTGNITNNRR184 pKa = 11.84AVLTEE189 pKa = 4.11SATVALTLDD198 pKa = 3.95GNVTGTYY205 pKa = 10.24SGISDD210 pKa = 3.63QVGDD214 pKa = 3.88VYY216 pKa = 11.62LEE218 pKa = 3.98VWDD221 pKa = 4.97GALHH225 pKa = 6.7PNGTVQLGHH234 pKa = 6.83VDD236 pKa = 3.39VDD238 pKa = 3.96SDD240 pKa = 4.0TQGAQAGPNGTGAFLFEE257 pKa = 4.37EE258 pKa = 4.42SGEE261 pKa = 3.93IDD263 pKa = 3.96LAGTLTGTVPLVSLQQAVNNSATVTGSLNGASGNVGLNVAAGSGNQQSNSLAIAAGCNACSGNGSPTGLL332 pKa = 3.59

Molecular weight: 32.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B3BMW2|A0A0B3BMW2_9PSED Protein kinase OS=Pseudomonas flexibilis OX=706570 GN=PT85_07140 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTLKK11 pKa = 10.31RR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.83GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3275

0

3275

1064546

38

3731

325.1

35.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.493 ± 0.054

1.015 ± 0.016

5.255 ± 0.029

6.192 ± 0.041

3.493 ± 0.029

8.132 ± 0.044

2.298 ± 0.02

4.417 ± 0.037

2.864 ± 0.042

12.53 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.202 ± 0.024

2.651 ± 0.024

4.993 ± 0.035

4.574 ± 0.037

7.416 ± 0.046

5.179 ± 0.03

4.472 ± 0.029

6.956 ± 0.036

1.438 ± 0.02

2.429 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski