Amino acid dipepetide frequency for Pseudomonas flexibilis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.54AlaAla: 13.54 ± 0.172	1.34AlaCys: 1.34 ± 0.039	6.004AlaAsp: 6.004 ± 0.075	8.114AlaGlu: 8.114 ± 0.109	3.733AlaPhe: 3.733 ± 0.067	9.785AlaGly: 9.785 ± 0.154	2.239AlaHis: 2.239 ± 0.05	5.048AlaIle: 5.048 ± 0.071	2.981AlaLys: 2.981 ± 0.066	15.139AlaLeu: 15.139 ± 0.193	2.821AlaMet: 2.821 ± 0.049	2.665AlaAsn: 2.665 ± 0.06	4.801AlaPro: 4.801 ± 0.082	5.2AlaGln: 5.2 ± 0.075	8.878AlaArg: 8.878 ± 0.101	6.035AlaSer: 6.035 ± 0.088	4.726AlaThr: 4.726 ± 0.077	7.636AlaVal: 7.636 ± 0.093	1.774AlaTrp: 1.774 ± 0.043	2.471AlaTyr: 2.471 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	1.22CysAla: 1.22 ± 0.041	0.142CysCys: 0.142 ± 0.013	0.5CysAsp: 0.5 ± 0.022	0.577CysGlu: 0.577 ± 0.022	0.336CysPhe: 0.336 ± 0.016	1.052CysGly: 1.052 ± 0.033	0.294CysHis: 0.294 ± 0.017	0.409CysIle: 0.409 ± 0.02	0.244CysLys: 0.244 ± 0.016	1.144CysLeu: 1.144 ± 0.037	0.186CysMet: 0.186 ± 0.014	0.247CysAsn: 0.247 ± 0.015	0.538CysPro: 0.538 ± 0.025	0.435CysGln: 0.435 ± 0.022	0.757CysArg: 0.757 ± 0.028	0.556CysSer: 0.556 ± 0.023	0.416CysThr: 0.416 ± 0.021	0.691CysVal: 0.691 ± 0.025	0.146CysTrp: 0.146 ± 0.01	0.256CysTyr: 0.256 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	5.971AspAla: 5.971 ± 0.075	0.547AspCys: 0.547 ± 0.023	2.709AspAsp: 2.709 ± 0.052	3.687AspGlu: 3.687 ± 0.066	1.988AspPhe: 1.988 ± 0.044	4.425AspGly: 4.425 ± 0.077	1.089AspHis: 1.089 ± 0.039	2.468AspIle: 2.468 ± 0.057	1.637AspLys: 1.637 ± 0.047	6.048AspLeu: 6.048 ± 0.07	1.154AspMet: 1.154 ± 0.032	1.422AspAsn: 1.422 ± 0.053	2.926AspPro: 2.926 ± 0.058	2.011AspGln: 2.011 ± 0.044	3.321AspArg: 3.321 ± 0.052	2.734AspSer: 2.734 ± 0.053	2.307AspThr: 2.307 ± 0.048	3.338AspVal: 3.338 ± 0.064	1.074AspTrp: 1.074 ± 0.034	1.701AspTyr: 1.701 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	7.46GluAla: 7.46 ± 0.101	0.472GluCys: 0.472 ± 0.021	2.682GluAsp: 2.682 ± 0.059	3.661GluGlu: 3.661 ± 0.061	1.787GluPhe: 1.787 ± 0.048	4.27GluGly: 4.27 ± 0.066	1.674GluHis: 1.674 ± 0.042	2.82GluIle: 2.82 ± 0.059	1.888GluLys: 1.888 ± 0.052	7.809GluLeu: 7.809 ± 0.1	1.335GluMet: 1.335 ± 0.037	1.492GluAsn: 1.492 ± 0.037	2.823GluPro: 2.823 ± 0.051	4.246GluGln: 4.246 ± 0.084	5.996GluArg: 5.996 ± 0.077	2.537GluSer: 2.537 ± 0.046	2.642GluThr: 2.642 ± 0.051	4.337GluVal: 4.337 ± 0.066	0.742GluTrp: 0.742 ± 0.029	1.251GluTyr: 1.251 ± 0.038	0.0GluXaa: 0.0 ± 0.0
Phe	4.157PheAla: 4.157 ± 0.07	0.419PheCys: 0.419 ± 0.021	2.311PheAsp: 2.311 ± 0.049	2.046PheGlu: 2.046 ± 0.05	1.4PhePhe: 1.4 ± 0.043	3.223PheGly: 3.223 ± 0.061	0.774PheHis: 0.774 ± 0.027	1.664PheIle: 1.664 ± 0.042	1.013PheLys: 1.013 ± 0.034	3.432PheLeu: 3.432 ± 0.068	0.751PheMet: 0.751 ± 0.027	1.113PheAsn: 1.113 ± 0.033	1.469PhePro: 1.469 ± 0.043	1.232PheGln: 1.232 ± 0.035	2.039PheArg: 2.039 ± 0.044	2.239PheSer: 2.239 ± 0.048	1.76PheThr: 1.76 ± 0.04	2.415PheVal: 2.415 ± 0.046	0.546PheTrp: 0.546 ± 0.023	0.924PheTyr: 0.924 ± 0.025	0.0PheXaa: 0.0 ± 0.0
Gly	7.793GlyAla: 7.793 ± 0.099	0.995GlyCys: 0.995 ± 0.032	3.945GlyAsp: 3.945 ± 0.075	5.488GlyGlu: 5.488 ± 0.076	3.308GlyPhe: 3.308 ± 0.06	6.299GlyGly: 6.299 ± 0.107	1.893GlyHis: 1.893 ± 0.046	4.041GlyIle: 4.041 ± 0.073	3.002GlyLys: 3.002 ± 0.072	10.023GlyLeu: 10.023 ± 0.131	2.242GlyMet: 2.242 ± 0.051	2.299GlyAsn: 2.299 ± 0.059	2.654GlyPro: 2.654 ± 0.055	3.769GlyGln: 3.769 ± 0.08	5.709GlyArg: 5.709 ± 0.068	4.317GlySer: 4.317 ± 0.073	3.686GlyThr: 3.686 ± 0.068	6.067GlyVal: 6.067 ± 0.081	1.458GlyTrp: 1.458 ± 0.042	2.335GlyTyr: 2.335 ± 0.046	0.0GlyXaa: 0.0 ± 0.0
His	2.407HisAla: 2.407 ± 0.05	0.342HisCys: 0.342 ± 0.018	1.199HisAsp: 1.199 ± 0.033	1.202HisGlu: 1.202 ± 0.037	0.976HisPhe: 0.976 ± 0.025	2.125HisGly: 2.125 ± 0.05	0.569HisHis: 0.569 ± 0.025	0.949HisIle: 0.949 ± 0.036	0.596HisLys: 0.596 ± 0.022	2.693HisLeu: 2.693 ± 0.06	0.515HisMet: 0.515 ± 0.025	0.602HisAsn: 0.602 ± 0.026	1.548HisPro: 1.548 ± 0.043	0.959HisGln: 0.959 ± 0.032	1.562HisArg: 1.562 ± 0.039	1.183HisSer: 1.183 ± 0.036	1.002HisThr: 1.002 ± 0.035	1.325HisVal: 1.325 ± 0.035	0.475HisTrp: 0.475 ± 0.024	0.756HisTyr: 0.756 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.535IleAla: 5.535 ± 0.088	0.419IleCys: 0.419 ± 0.02	2.978IleAsp: 2.978 ± 0.055	3.21IleGlu: 3.21 ± 0.062	1.306IlePhe: 1.306 ± 0.039	4.294IleGly: 4.294 ± 0.069	1.022IleHis: 1.022 ± 0.027	1.793IleIle: 1.793 ± 0.054	1.375IleLys: 1.375 ± 0.044	4.181IleLeu: 4.181 ± 0.069	0.787IleMet: 0.787 ± 0.032	1.464IleAsn: 1.464 ± 0.04	2.153IlePro: 2.153 ± 0.053	1.603IleGln: 1.603 ± 0.043	3.076IleArg: 3.076 ± 0.059	2.391IleSer: 2.391 ± 0.054	2.253IleThr: 2.253 ± 0.054	2.897IleVal: 2.897 ± 0.058	0.444IleTrp: 0.444 ± 0.021	0.986IleTyr: 0.986 ± 0.03	0.0IleXaa: 0.0 ± 0.0
Lys	3.485LysAla: 3.485 ± 0.074	0.181LysCys: 0.181 ± 0.014	1.431LysAsp: 1.431 ± 0.036	1.445LysGlu: 1.445 ± 0.041	0.775LysPhe: 0.775 ± 0.032	2.291LysGly: 2.291 ± 0.05	0.659LysHis: 0.659 ± 0.027	1.308LysIle: 1.308 ± 0.039	1.124LysLys: 1.124 ± 0.04	3.109LysLeu: 3.109 ± 0.064	0.594LysMet: 0.594 ± 0.024	0.801LysAsn: 0.801 ± 0.031	1.74LysPro: 1.74 ± 0.052	1.278LysGln: 1.278 ± 0.044	2.155LysArg: 2.155 ± 0.054	1.502LysSer: 1.502 ± 0.042	1.514LysThr: 1.514 ± 0.039	2.38LysVal: 2.38 ± 0.051	0.305LysTrp: 0.305 ± 0.016	0.565LysTyr: 0.565 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	16.067LeuAla: 16.067 ± 0.19	1.323LeuCys: 1.323 ± 0.039	7.274LeuAsp: 7.274 ± 0.08	7.313LeuGlu: 7.313 ± 0.091	4.381LeuPhe: 4.381 ± 0.092	10.18LeuGly: 10.18 ± 0.131	2.705LeuHis: 2.705 ± 0.055	5.308LeuIle: 5.308 ± 0.076	3.822LeuLys: 3.822 ± 0.065	15.597LeuLeu: 15.597 ± 0.239	2.528LeuMet: 2.528 ± 0.047	3.332LeuAsn: 3.332 ± 0.059	6.893LeuPro: 6.893 ± 0.093	4.918LeuGln: 4.918 ± 0.086	8.9LeuArg: 8.9 ± 0.116	6.665LeuSer: 6.665 ± 0.09	5.565LeuThr: 5.565 ± 0.077	8.306LeuVal: 8.306 ± 0.106	1.505LeuTrp: 1.505 ± 0.041	2.714LeuTyr: 2.714 ± 0.054	0.0LeuXaa: 0.0 ± 0.0
Met	2.607MetAla: 2.607 ± 0.056	0.165MetCys: 0.165 ± 0.012	0.976MetAsp: 0.976 ± 0.03	1.032MetGlu: 1.032 ± 0.031	0.626MetPhe: 0.626 ± 0.027	1.655MetGly: 1.655 ± 0.045	0.5MetHis: 0.5 ± 0.019	0.995MetIle: 0.995 ± 0.032	0.795MetLys: 0.795 ± 0.027	2.667MetLeu: 2.667 ± 0.058	0.484MetMet: 0.484 ± 0.021	0.777MetAsn: 0.777 ± 0.026	1.324MetPro: 1.324 ± 0.04	1.015MetGln: 1.015 ± 0.035	1.567MetArg: 1.567 ± 0.038	1.56MetSer: 1.56 ± 0.036	1.294MetThr: 1.294 ± 0.033	1.481MetVal: 1.481 ± 0.043	0.152MetTrp: 0.152 ± 0.011	0.345MetTyr: 0.345 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	2.92AsnAla: 2.92 ± 0.057	0.277AsnCys: 0.277 ± 0.017	1.36AsnAsp: 1.36 ± 0.041	1.387AsnGlu: 1.387 ± 0.039	0.886AsnPhe: 0.886 ± 0.027	2.263AsnGly: 2.263 ± 0.055	0.605AsnHis: 0.605 ± 0.026	1.222AsnIle: 1.222 ± 0.034	0.724AsnLys: 0.724 ± 0.027	3.315AsnLeu: 3.315 ± 0.063	0.531AsnMet: 0.531 ± 0.026	0.712AsnAsn: 0.712 ± 0.028	1.891AsnPro: 1.891 ± 0.039	1.145AsnGln: 1.145 ± 0.039	1.991AsnArg: 1.991 ± 0.053	1.279AsnSer: 1.279 ± 0.039	1.203AsnThr: 1.203 ± 0.039	1.717AsnVal: 1.717 ± 0.042	0.361AsnTrp: 0.361 ± 0.021	0.717AsnTyr: 0.717 ± 0.031	0.0AsnXaa: 0.0 ± 0.0
Pro	6.205ProAla: 6.205 ± 0.088	0.412ProCys: 0.412 ± 0.021	2.942ProAsp: 2.942 ± 0.055	3.684ProGlu: 3.684 ± 0.062	1.8ProPhe: 1.8 ± 0.039	4.272ProGly: 4.272 ± 0.065	1.146ProHis: 1.146 ± 0.033	1.799ProIle: 1.799 ± 0.043	1.245ProLys: 1.245 ± 0.036	6.259ProLeu: 6.259 ± 0.086	1.138ProMet: 1.138 ± 0.033	1.258ProAsn: 1.258 ± 0.033	2.133ProPro: 2.133 ± 0.051	2.261ProGln: 2.261 ± 0.047	3.105ProArg: 3.105 ± 0.061	2.397ProSer: 2.397 ± 0.05	2.042ProThr: 2.042 ± 0.042	3.802ProVal: 3.802 ± 0.063	0.821ProTrp: 0.821 ± 0.029	1.214ProTyr: 1.214 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	6.395GlnAla: 6.395 ± 0.105	0.319GlnCys: 0.319 ± 0.013	1.857GlnAsp: 1.857 ± 0.042	2.136GlnGlu: 2.136 ± 0.05	1.321GlnPhe: 1.321 ± 0.038	3.476GlnGly: 3.476 ± 0.065	1.11GlnHis: 1.11 ± 0.039	1.87GlnIle: 1.87 ± 0.05	1.053GlnLys: 1.053 ± 0.033	5.725GlnLeu: 5.725 ± 0.091	0.974GlnMet: 0.974 ± 0.034	0.922GlnAsn: 0.922 ± 0.031	2.679GlnPro: 2.679 ± 0.061	2.635GlnGln: 2.635 ± 0.071	4.212GlnArg: 4.212 ± 0.077	1.971GlnSer: 1.971 ± 0.046	1.883GlnThr: 1.883 ± 0.049	3.653GlnVal: 3.653 ± 0.07	0.615GlnTrp: 0.615 ± 0.023	0.932GlnTyr: 0.932 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	7.173ArgAla: 7.173 ± 0.099	0.726ArgCys: 0.726 ± 0.03	3.988ArgAsp: 3.988 ± 0.073	5.163ArgGlu: 5.163 ± 0.08	2.991ArgPhe: 2.991 ± 0.058	4.743ArgGly: 4.743 ± 0.069	2.094ArgHis: 2.094 ± 0.045	3.637ArgIle: 3.637 ± 0.062	1.939ArgLys: 1.939 ± 0.052	10.383ArgLeu: 10.383 ± 0.14	1.745ArgMet: 1.745 ± 0.045	1.931ArgAsn: 1.931 ± 0.044	3.297ArgPro: 3.297 ± 0.062	4.176ArgGln: 4.176 ± 0.071	5.402ArgArg: 5.402 ± 0.091	3.468ArgSer: 3.468 ± 0.055	2.898ArgThr: 2.898 ± 0.065	5.049ArgVal: 5.049 ± 0.077	1.128ArgTrp: 1.128 ± 0.033	2.226ArgTyr: 2.226 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	5.597SerAla: 5.597 ± 0.077	0.466SerCys: 0.466 ± 0.023	2.477SerAsp: 2.477 ± 0.046	2.958SerGlu: 2.958 ± 0.049	1.906SerPhe: 1.906 ± 0.046	4.889SerGly: 4.889 ± 0.08	1.207SerHis: 1.207 ± 0.032	2.278SerIle: 2.278 ± 0.045	1.299SerLys: 1.299 ± 0.033	6.996SerLeu: 6.996 ± 0.107	1.145SerMet: 1.145 ± 0.031	1.372SerAsn: 1.372 ± 0.041	2.479SerPro: 2.479 ± 0.046	2.224SerGln: 2.224 ± 0.052	3.71SerArg: 3.71 ± 0.059	2.744SerSer: 2.744 ± 0.066	2.409SerThr: 2.409 ± 0.051	3.57SerVal: 3.57 ± 0.064	0.762SerTrp: 0.762 ± 0.028	1.302SerTyr: 1.302 ± 0.034	0.0SerXaa: 0.0 ± 0.0
Thr	4.811ThrAla: 4.811 ± 0.085	0.448ThrCys: 0.448 ± 0.023	2.209ThrAsp: 2.209 ± 0.047	2.321ThrGlu: 2.321 ± 0.046	1.583ThrPhe: 1.583 ± 0.04	4.037ThrGly: 4.037 ± 0.066	1.005ThrHis: 1.005 ± 0.029	1.633ThrIle: 1.633 ± 0.041	0.869ThrLys: 0.869 ± 0.031	6.861ThrLeu: 6.861 ± 0.096	0.689ThrMet: 0.689 ± 0.026	1.03ThrAsn: 1.03 ± 0.036	3.053ThrPro: 3.053 ± 0.055	1.727ThrGln: 1.727 ± 0.045	3.303ThrArg: 3.303 ± 0.053	2.211ThrSer: 2.211 ± 0.049	2.052ThrThr: 2.052 ± 0.048	3.182ThrVal: 3.182 ± 0.06	0.604ThrTrp: 0.604 ± 0.025	1.09ThrTyr: 1.09 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	7.793ValAla: 7.793 ± 0.101	0.71ValCys: 0.71 ± 0.028	3.82ValAsp: 3.82 ± 0.064	4.639ValGlu: 4.639 ± 0.075	2.433ValPhe: 2.433 ± 0.056	5.146ValGly: 5.146 ± 0.082	1.437ValHis: 1.437 ± 0.037	3.49ValIle: 3.49 ± 0.068	1.995ValLys: 1.995 ± 0.054	8.39ValLeu: 8.39 ± 0.11	1.63ValMet: 1.63 ± 0.042	2.01ValAsn: 2.01 ± 0.046	3.522ValPro: 3.522 ± 0.062	2.672ValGln: 2.672 ± 0.048	4.961ValArg: 4.961 ± 0.065	3.884ValSer: 3.884 ± 0.06	3.416ValThr: 3.416 ± 0.061	5.285ValVal: 5.285 ± 0.09	0.829ValTrp: 0.829 ± 0.031	1.495ValTyr: 1.495 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	1.202TrpAla: 1.202 ± 0.035	0.162TrpCys: 0.162 ± 0.012	0.613TrpAsp: 0.613 ± 0.03	0.666TrpGlu: 0.666 ± 0.024	0.506TrpPhe: 0.506 ± 0.018	0.876TrpGly: 0.876 ± 0.032	0.399TrpHis: 0.399 ± 0.019	0.593TrpIle: 0.593 ± 0.024	0.421TrpLys: 0.421 ± 0.023	2.45TrpLeu: 2.45 ± 0.062	0.347TrpMet: 0.347 ± 0.018	0.415TrpAsn: 0.415 ± 0.02	0.701TrpPro: 0.701 ± 0.024	0.936TrpGln: 0.936 ± 0.036	1.267TrpArg: 1.267 ± 0.039	0.779TrpSer: 0.779 ± 0.031	0.538TrpThr: 0.538 ± 0.023	0.926TrpVal: 0.926 ± 0.03	0.24TrpTrp: 0.24 ± 0.017	0.348TrpTyr: 0.348 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.593TyrAla: 2.593 ± 0.053	0.283TyrCys: 0.283 ± 0.016	1.278TyrAsp: 1.278 ± 0.036	1.194TyrGlu: 1.194 ± 0.035	0.887TyrPhe: 0.887 ± 0.032	2.022TyrGly: 2.022 ± 0.042	0.563TyrHis: 0.563 ± 0.024	0.844TyrIle: 0.844 ± 0.03	0.621TyrLys: 0.621 ± 0.027	3.074TyrLeu: 3.074 ± 0.058	0.423TyrMet: 0.423 ± 0.02	0.641TyrAsn: 0.641 ± 0.026	1.31TyrPro: 1.31 ± 0.033	1.297TyrGln: 1.297 ± 0.035	2.249TyrArg: 2.249 ± 0.051	1.339TyrSer: 1.339 ± 0.039	1.109TyrThr: 1.109 ± 0.039	1.501TyrVal: 1.501 ± 0.036	0.403TyrTrp: 0.403 ± 0.022	0.666TyrTyr: 0.666 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3275 proteins (1064546 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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