Sanguibacteroides justesenii
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2587 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C3NCV7|A0A0C3NCV7_9PORP Uncharacterized protein OS=Sanguibacteroides justesenii OX=1547597 GN=BA92_11030 PE=4 SV=1
MM1 pKa = 7.57 EE2 pKa = 4.96 ATTLSAARR10 pKa = 11.84 VYY12 pKa = 10.74 VGTYY16 pKa = 9.3 GKK18 pKa = 10.57 YY19 pKa = 9.55 NAGSLFGEE27 pKa = 4.2 WLDD30 pKa = 4.65 LSDD33 pKa = 4.74 YY34 pKa = 10.89 SDD36 pKa = 3.83 KK37 pKa = 11.69 EE38 pKa = 4.08 EE39 pKa = 5.23 FYY41 pKa = 10.83 DD42 pKa = 4.63 ACRR45 pKa = 11.84 EE46 pKa = 4.06 LHH48 pKa = 6.7 KK49 pKa = 11.06 DD50 pKa = 3.35 EE51 pKa = 4.97 EE52 pKa = 4.59 DD53 pKa = 3.7 AEE55 pKa = 5.3 FMFQDD60 pKa = 3.7 WEE62 pKa = 4.34 NVPEE66 pKa = 3.97 NLIGEE71 pKa = 4.47 SWLSEE76 pKa = 3.86 NFFALRR82 pKa = 11.84 DD83 pKa = 3.79 AVEE86 pKa = 4.52 DD87 pKa = 4.18 LGDD90 pKa = 3.76 TEE92 pKa = 4.54 QEE94 pKa = 5.05 AFFVWCNYY102 pKa = 9.32 KK103 pKa = 10.32 SHH105 pKa = 7.73 DD106 pKa = 4.59 LGEE109 pKa = 4.33 EE110 pKa = 3.98 DD111 pKa = 5.6 ADD113 pKa = 4.0 DD114 pKa = 3.91 LVRR117 pKa = 11.84 DD118 pKa = 4.71 FRR120 pKa = 11.84 DD121 pKa = 3.81 EE122 pKa = 4.08 YY123 pKa = 10.55 QGQYY127 pKa = 11.06 DD128 pKa = 3.94 DD129 pKa = 5.56 EE130 pKa = 5.07 EE131 pKa = 5.7 DD132 pKa = 3.22 FAYY135 pKa = 10.08 EE136 pKa = 4.43 IIEE139 pKa = 4.11 EE140 pKa = 4.66 CYY142 pKa = 10.66 DD143 pKa = 3.52 LPEE146 pKa = 4.02 FAKK149 pKa = 10.18 TYY151 pKa = 10.62 FDD153 pKa = 3.75 YY154 pKa = 11.44 EE155 pKa = 4.0 KK156 pKa = 10.43 FARR159 pKa = 11.84 DD160 pKa = 5.04 LFMCDD165 pKa = 2.56 YY166 pKa = 10.58 WMDD169 pKa = 3.26 EE170 pKa = 4.02 GFVFRR175 pKa = 11.84 AAA177 pKa = 3.78
Molecular weight: 21.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.757
IPC_protein 3.757
Toseland 3.554
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.643
Rodwell 3.579
Grimsley 3.452
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 4.037
Thurlkill 3.592
EMBOSS 3.656
Sillero 3.872
Patrickios 0.718
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.768
Protein with the highest isoelectric point:
>tr|A0A0C3N9K5|A0A0C3N9K5_9PORP YhcH/YjgK/YiaL family protein OS=Sanguibacteroides justesenii OX=1547597 GN=BA92_14290 PE=4 SV=1
MM1 pKa = 7.67 IGRR4 pKa = 11.84 IFILDD9 pKa = 4.04 GKK11 pKa = 9.86 VLDD14 pKa = 4.02 YY15 pKa = 11.6 GRR17 pKa = 11.84 FALLLHH23 pKa = 6.71 SYY25 pKa = 10.55 RR26 pKa = 11.84 RR27 pKa = 11.84 MKK29 pKa = 10.37 RR30 pKa = 11.84 ICALLSVDD38 pKa = 3.63 VLILGRR44 pKa = 11.84 YY45 pKa = 9.07 IIGKK49 pKa = 9.17 RR50 pKa = 11.84 RR51 pKa = 11.84 NLCLDD56 pKa = 3.14 YY57 pKa = 10.75 RR58 pKa = 11.84 RR59 pKa = 11.84 SKK61 pKa = 10.92 AGSNNEE67 pKa = 3.77
Molecular weight: 7.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.336
IPC2_protein 9.809
IPC_protein 10.643
Toseland 10.511
ProMoST 10.292
Dawson 10.672
Bjellqvist 10.423
Wikipedia 10.906
Rodwell 10.847
Grimsley 10.745
Solomon 10.774
Lehninger 10.73
Nozaki 10.526
DTASelect 10.409
Thurlkill 10.555
EMBOSS 10.921
Sillero 10.599
Patrickios 10.599
IPC_peptide 10.76
IPC2_peptide 9.575
IPC2.peptide.svr19 8.353
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2587
0
2587
944104
58
2245
364.9
41.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.413 ± 0.047
1.116 ± 0.018
5.293 ± 0.031
6.919 ± 0.047
4.871 ± 0.035
6.8 ± 0.038
1.735 ± 0.019
7.541 ± 0.037
6.931 ± 0.044
9.438 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.648 ± 0.022
4.871 ± 0.038
3.677 ± 0.023
3.133 ± 0.027
4.986 ± 0.036
6.002 ± 0.034
5.313 ± 0.031
6.746 ± 0.036
1.149 ± 0.015
4.421 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here