Methyloprofundus sedimenti

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales; Methylococcaceae; Methyloprofundus

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3608 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V8M7H1|A0A1V8M7H1_9GAMM 30S ribosomal protein S1 OS=Methyloprofundus sedimenti OX=1420851 GN=rpsA PE=3 SV=1
MM1 pKa = 7.36IVYY4 pKa = 8.07PKK6 pKa = 10.43QIDD9 pKa = 3.83GLEE12 pKa = 4.26PSPADD17 pKa = 4.03DD18 pKa = 4.09GFLIYY23 pKa = 10.34FQDD26 pKa = 3.74TDD28 pKa = 3.3RR29 pKa = 11.84VHH31 pKa = 6.2YY32 pKa = 9.83LNHH35 pKa = 6.49IATLVLLLCDD45 pKa = 4.15GKK47 pKa = 10.71NSNEE51 pKa = 4.94DD52 pKa = 3.22IPKK55 pKa = 10.39LLQQQYY61 pKa = 10.14DD62 pKa = 3.92LPEE65 pKa = 5.38APIDD69 pKa = 5.04DD70 pKa = 3.9VDD72 pKa = 3.79ALLNQFVDD80 pKa = 3.23EE81 pKa = 4.66GLIVLNVTTSAA92 pKa = 3.83

Molecular weight: 10.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V8M6L9|A0A1V8M6L9_9GAMM Peptidoglycan D D-transpeptidase FtsI OS=Methyloprofundus sedimenti OX=1420851 GN=ftsI PE=3 SV=1
MM1 pKa = 7.63LLTVVVYY8 pKa = 9.0FRR10 pKa = 11.84GRR12 pKa = 11.84AKK14 pKa = 10.17RR15 pKa = 11.84RR16 pKa = 11.84EE17 pKa = 3.93AGRR20 pKa = 11.84PVSIGQTITFLLGMLCFVIALQSPLEE46 pKa = 4.07PLSEE50 pKa = 4.08HH51 pKa = 6.57FLFFHH56 pKa = 6.22QIEE59 pKa = 4.84HH60 pKa = 6.09ILMRR64 pKa = 11.84GLGPLLLILSMPLAPLLQGLPKK86 pKa = 9.99IIRR89 pKa = 11.84HH90 pKa = 5.27SVLTPIIRR98 pKa = 11.84NKK100 pKa = 8.65PVQWLYY106 pKa = 11.61KK107 pKa = 10.2FLGHH111 pKa = 6.72PAIASILFIATLLIWQIPSLHH132 pKa = 5.82NRR134 pKa = 11.84ALANQSLHH142 pKa = 5.07NWMHH146 pKa = 5.85FTMILTGFFFWWLICDD162 pKa = 3.68PRR164 pKa = 11.84QNTSRR169 pKa = 11.84IPFGMRR175 pKa = 11.84IVILWLVTIPNTIIGAMITLKK196 pKa = 10.32RR197 pKa = 11.84DD198 pKa = 3.44QIYY201 pKa = 10.47AAYY204 pKa = 9.97DD205 pKa = 3.46VLDD208 pKa = 3.72GRR210 pKa = 11.84LAIEE214 pKa = 4.8IMLDD218 pKa = 3.45QQLGGIMIWGPGGMMGLIGTAVVFFLWTRR247 pKa = 11.84KK248 pKa = 9.03DD249 pKa = 3.18RR250 pKa = 11.84KK251 pKa = 9.92LRR253 pKa = 11.84YY254 pKa = 9.54AKK256 pKa = 10.61

Molecular weight: 29.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3608

0

3608

1120843

50

2923

310.7

34.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.416 ± 0.042

1.133 ± 0.016

5.371 ± 0.027

5.955 ± 0.04

4.253 ± 0.028

6.51 ± 0.043

2.375 ± 0.024

7.282 ± 0.034

5.682 ± 0.047

10.633 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.471 ± 0.019

4.375 ± 0.029

3.958 ± 0.027

4.805 ± 0.037

4.451 ± 0.03

6.312 ± 0.032

5.177 ± 0.028

6.372 ± 0.037

1.256 ± 0.017

3.212 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski