Amino acid dipepetide frequency for Methyloprofundus sedimenti

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.328AlaAla: 7.328 ± 0.108
1.076AlaCys: 1.076 ± 0.034
4.695AlaAsp: 4.695 ± 0.073
5.817AlaGlu: 5.817 ± 0.073
3.299AlaPhe: 3.299 ± 0.056
5.822AlaGly: 5.822 ± 0.086
1.779AlaHis: 1.779 ± 0.04
6.249AlaIle: 6.249 ± 0.072
4.77AlaLys: 4.77 ± 0.074
9.443AlaLeu: 9.443 ± 0.109
2.427AlaMet: 2.427 ± 0.057
3.407AlaAsn: 3.407 ± 0.069
2.642AlaPro: 2.642 ± 0.056
3.677AlaGln: 3.677 ± 0.062
3.552AlaArg: 3.552 ± 0.061
4.838AlaSer: 4.838 ± 0.067
4.162AlaThr: 4.162 ± 0.058
5.549AlaVal: 5.549 ± 0.085
1.055AlaTrp: 1.055 ± 0.032
2.575AlaTyr: 2.575 ± 0.052
0.0AlaXaa: 0.0 ± 0.0
Cys
0.909CysAla: 0.909 ± 0.028
0.183CysCys: 0.183 ± 0.013
0.543CysAsp: 0.543 ± 0.026
0.551CysGlu: 0.551 ± 0.025
0.511CysPhe: 0.511 ± 0.022
0.902CysGly: 0.902 ± 0.032
0.438CysHis: 0.438 ± 0.032
0.804CysIle: 0.804 ± 0.029
0.519CysLys: 0.519 ± 0.022
1.195CysLeu: 1.195 ± 0.036
0.223CysMet: 0.223 ± 0.014
0.403CysAsn: 0.403 ± 0.021
0.543CysPro: 0.543 ± 0.026
0.488CysGln: 0.488 ± 0.022
0.513CysArg: 0.513 ± 0.024
0.804CysSer: 0.804 ± 0.028
0.602CysThr: 0.602 ± 0.025
0.674CysVal: 0.674 ± 0.024
0.127CysTrp: 0.127 ± 0.011
0.399CysTyr: 0.399 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
4.332AspAla: 4.332 ± 0.071
0.654AspCys: 0.654 ± 0.031
2.786AspAsp: 2.786 ± 0.059
3.341AspGlu: 3.341 ± 0.055
2.699AspPhe: 2.699 ± 0.049
3.116AspGly: 3.116 ± 0.057
1.076AspHis: 1.076 ± 0.029
4.39AspIle: 4.39 ± 0.065
3.358AspLys: 3.358 ± 0.055
5.453AspLeu: 5.453 ± 0.067
1.321AspMet: 1.321 ± 0.032
2.409AspAsn: 2.409 ± 0.049
2.109AspPro: 2.109 ± 0.05
2.231AspGln: 2.231 ± 0.045
2.209AspArg: 2.209 ± 0.045
3.214AspSer: 3.214 ± 0.058
2.655AspThr: 2.655 ± 0.051
3.289AspVal: 3.289 ± 0.055
0.907AspTrp: 0.907 ± 0.031
2.161AspTyr: 2.161 ± 0.045
0.0AspXaa: 0.0 ± 0.0
Glu
4.564GluAla: 4.564 ± 0.058
0.51GluCys: 0.51 ± 0.025
2.718GluAsp: 2.718 ± 0.053
3.24GluGlu: 3.24 ± 0.056
2.315GluPhe: 2.315 ± 0.047
3.326GluGly: 3.326 ± 0.067
1.573GluHis: 1.573 ± 0.036
4.555GluIle: 4.555 ± 0.071
4.035GluLys: 4.035 ± 0.071
6.629GluLeu: 6.629 ± 0.088
1.59GluMet: 1.59 ± 0.036
2.935GluAsn: 2.935 ± 0.049
1.894GluPro: 1.894 ± 0.037
4.043GluGln: 4.043 ± 0.066
2.935GluArg: 2.935 ± 0.063
3.319GluSer: 3.319 ± 0.063
3.074GluThr: 3.074 ± 0.064
3.749GluVal: 3.749 ± 0.066
0.66GluTrp: 0.66 ± 0.025
1.885GluTyr: 1.885 ± 0.042
0.0GluXaa: 0.0 ± 0.0
Phe
3.499PheAla: 3.499 ± 0.055
0.577PheCys: 0.577 ± 0.025
2.554PheAsp: 2.554 ± 0.047
2.149PheGlu: 2.149 ± 0.043
2.021PhePhe: 2.021 ± 0.049
2.792PheGly: 2.792 ± 0.056
0.877PheHis: 0.877 ± 0.03
3.32PheIle: 3.32 ± 0.068
2.236PheLys: 2.236 ± 0.049
4.053PheLeu: 4.053 ± 0.069
1.058PheMet: 1.058 ± 0.033
2.167PheAsn: 2.167 ± 0.048
1.641PhePro: 1.641 ± 0.035
1.504PheGln: 1.504 ± 0.038
1.637PheArg: 1.637 ± 0.038
3.464PheSer: 3.464 ± 0.06
2.324PheThr: 2.324 ± 0.047
2.554PheVal: 2.554 ± 0.05
0.595PheTrp: 0.595 ± 0.026
1.511PheTyr: 1.511 ± 0.043
0.0PheXaa: 0.0 ± 0.0
Gly
4.846GlyAla: 4.846 ± 0.078
0.914GlyCys: 0.914 ± 0.029
3.293GlyAsp: 3.293 ± 0.062
3.747GlyGlu: 3.747 ± 0.06
3.24GlyPhe: 3.24 ± 0.048
4.489GlyGly: 4.489 ± 0.081
1.517GlyHis: 1.517 ± 0.044
5.162GlyIle: 5.162 ± 0.078
4.091GlyLys: 4.091 ± 0.065
6.896GlyLeu: 6.896 ± 0.093
1.908GlyMet: 1.908 ± 0.051
2.634GlyAsn: 2.634 ± 0.054
1.667GlyPro: 1.667 ± 0.038
2.561GlyGln: 2.561 ± 0.053
2.975GlyArg: 2.975 ± 0.057
3.905GlySer: 3.905 ± 0.064
3.311GlyThr: 3.311 ± 0.061
4.567GlyVal: 4.567 ± 0.078
0.938GlyTrp: 0.938 ± 0.032
2.435GlyTyr: 2.435 ± 0.049
0.0GlyXaa: 0.0 ± 0.0
His
1.891HisAla: 1.891 ± 0.043
0.352HisCys: 0.352 ± 0.018
1.195HisAsp: 1.195 ± 0.029
1.308HisGlu: 1.308 ± 0.039
1.141HisPhe: 1.141 ± 0.036
1.518HisGly: 1.518 ± 0.041
0.724HisHis: 0.724 ± 0.026
1.742HisIle: 1.742 ± 0.04
1.342HisLys: 1.342 ± 0.037
2.493HisLeu: 2.493 ± 0.05
0.505HisMet: 0.505 ± 0.017
1.014HisAsn: 1.014 ± 0.031
1.159HisPro: 1.159 ± 0.034
1.167HisGln: 1.167 ± 0.029
1.058HisArg: 1.058 ± 0.032
1.373HisSer: 1.373 ± 0.035
1.131HisThr: 1.131 ± 0.028
1.309HisVal: 1.309 ± 0.034
0.394HisTrp: 0.394 ± 0.017
0.939HisTyr: 0.939 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
6.931IleAla: 6.931 ± 0.093
0.738IleCys: 0.738 ± 0.026
4.572IleAsp: 4.572 ± 0.065
4.832IleGlu: 4.832 ± 0.064
2.762IlePhe: 2.762 ± 0.052
4.644IleGly: 4.644 ± 0.07
1.552IleHis: 1.552 ± 0.036
5.176IleIle: 5.176 ± 0.074
4.352IleLys: 4.352 ± 0.07
6.573IleLeu: 6.573 ± 0.081
1.552IleMet: 1.552 ± 0.041
3.683IleAsn: 3.683 ± 0.06
3.25IlePro: 3.25 ± 0.053
2.852IleGln: 2.852 ± 0.053
3.092IleArg: 3.092 ± 0.059
5.143IleSer: 5.143 ± 0.069
3.936IleThr: 3.936 ± 0.062
4.324IleVal: 4.324 ± 0.063
0.718IleTrp: 0.718 ± 0.03
2.141IleTyr: 2.141 ± 0.044
0.0IleXaa: 0.0 ± 0.0
Lys
4.605LysAla: 4.605 ± 0.069
0.427LysCys: 0.427 ± 0.022
2.847LysAsp: 2.847 ± 0.051
3.272LysGlu: 3.272 ± 0.067
1.662LysPhe: 1.662 ± 0.04
3.362LysGly: 3.362 ± 0.055
1.411LysHis: 1.411 ± 0.032
4.258LysIle: 4.258 ± 0.058
3.697LysLys: 3.697 ± 0.08
5.665LysLeu: 5.665 ± 0.078
1.444LysMet: 1.444 ± 0.038
2.909LysAsn: 2.909 ± 0.06
2.433LysPro: 2.433 ± 0.052
3.526LysGln: 3.526 ± 0.07
2.682LysArg: 2.682 ± 0.051
3.432LysSer: 3.432 ± 0.062
3.362LysThr: 3.362 ± 0.065
3.622LysVal: 3.622 ± 0.057
0.559LysTrp: 0.559 ± 0.023
1.651LysTyr: 1.651 ± 0.038
0.0LysXaa: 0.0 ± 0.0
Leu
9.781LeuAla: 9.781 ± 0.092
1.224LeuCys: 1.224 ± 0.035
5.743LeuAsp: 5.743 ± 0.074
6.024LeuGlu: 6.024 ± 0.078
4.58LeuPhe: 4.58 ± 0.08
6.537LeuGly: 6.537 ± 0.093
2.345LeuHis: 2.345 ± 0.05
7.365LeuIle: 7.365 ± 0.104
5.998LeuLys: 5.998 ± 0.076
11.526LeuLeu: 11.526 ± 0.155
2.617LeuMet: 2.617 ± 0.053
4.859LeuAsn: 4.859 ± 0.068
4.807LeuPro: 4.807 ± 0.07
4.741LeuGln: 4.741 ± 0.071
4.522LeuArg: 4.522 ± 0.073
7.587LeuSer: 7.587 ± 0.096
5.653LeuThr: 5.653 ± 0.08
6.292LeuVal: 6.292 ± 0.084
1.166LeuTrp: 1.166 ± 0.03
2.967LeuTyr: 2.967 ± 0.052
0.0LeuXaa: 0.0 ± 0.0
Met
2.255MetAla: 2.255 ± 0.048
0.19MetCys: 0.19 ± 0.013
1.219MetAsp: 1.219 ± 0.031
1.183MetGlu: 1.183 ± 0.035
0.776MetPhe: 0.776 ± 0.028
1.699MetGly: 1.699 ± 0.04
0.606MetHis: 0.606 ± 0.025
1.622MetIle: 1.622 ± 0.041
1.297MetLys: 1.297 ± 0.034
2.72MetLeu: 2.72 ± 0.056
0.667MetMet: 0.667 ± 0.025
1.138MetAsn: 1.138 ± 0.033
1.298MetPro: 1.298 ± 0.036
1.541MetGln: 1.541 ± 0.037
1.191MetArg: 1.191 ± 0.032
1.725MetSer: 1.725 ± 0.038
1.457MetThr: 1.457 ± 0.04
1.439MetVal: 1.439 ± 0.036
0.159MetTrp: 0.159 ± 0.011
0.524MetTyr: 0.524 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
3.526AsnAla: 3.526 ± 0.054
0.488AsnCys: 0.488 ± 0.02
2.263AsnAsp: 2.263 ± 0.051
2.428AsnGlu: 2.428 ± 0.045
1.675AsnPhe: 1.675 ± 0.04
2.683AsnGly: 2.683 ± 0.052
1.049AsnHis: 1.049 ± 0.031
3.618AsnIle: 3.618 ± 0.054
2.776AsnLys: 2.776 ± 0.055
4.002AsnLeu: 4.002 ± 0.059
1.003AsnMet: 1.003 ± 0.031
2.21AsnAsn: 2.21 ± 0.049
2.178AsnPro: 2.178 ± 0.044
2.185AsnGln: 2.185 ± 0.05
1.863AsnArg: 1.863 ± 0.035
2.791AsnSer: 2.791 ± 0.055
2.474AsnThr: 2.474 ± 0.049
2.408AsnVal: 2.408 ± 0.051
0.634AsnTrp: 0.634 ± 0.022
1.498AsnTyr: 1.498 ± 0.037
0.0AsnXaa: 0.0 ± 0.0
Pro
3.349ProAla: 3.349 ± 0.057
0.363ProCys: 0.363 ± 0.017
2.699ProAsp: 2.699 ± 0.049
3.567ProGlu: 3.567 ± 0.061
1.784ProPhe: 1.784 ± 0.04
2.887ProGly: 2.887 ± 0.054
0.817ProHis: 0.817 ± 0.027
2.418ProIle: 2.418 ± 0.056
1.965ProLys: 1.965 ± 0.041
4.172ProLeu: 4.172 ± 0.063
0.889ProMet: 0.889 ± 0.024
1.445ProAsn: 1.445 ± 0.038
1.352ProPro: 1.352 ± 0.043
1.306ProGln: 1.306 ± 0.034
1.434ProArg: 1.434 ± 0.035
2.063ProSer: 2.063 ± 0.043
1.783ProThr: 1.783 ± 0.046
3.451ProVal: 3.451 ± 0.061
0.509ProTrp: 0.509 ± 0.021
1.326ProTyr: 1.326 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
4.515GlnAla: 4.515 ± 0.075
0.49GlnCys: 0.49 ± 0.021
2.184GlnAsp: 2.184 ± 0.045
2.609GlnGlu: 2.609 ± 0.049
1.806GlnPhe: 1.806 ± 0.046
3.039GlnGly: 3.039 ± 0.052
1.418GlnHis: 1.418 ± 0.036
3.054GlnIle: 3.054 ± 0.053
2.52GlnLys: 2.52 ± 0.052
5.333GlnLeu: 5.333 ± 0.08
1.039GlnMet: 1.039 ± 0.033
1.802GlnAsn: 1.802 ± 0.048
1.645GlnPro: 1.645 ± 0.04
3.781GlnGln: 3.781 ± 0.089
2.513GlnArg: 2.513 ± 0.051
2.722GlnSer: 2.722 ± 0.055
2.378GlnThr: 2.378 ± 0.047
3.074GlnVal: 3.074 ± 0.053
0.683GlnTrp: 0.683 ± 0.027
1.449GlnTyr: 1.449 ± 0.033
0.0GlnXaa: 0.0 ± 0.0
Arg
3.181ArgAla: 3.181 ± 0.053
0.487ArgCys: 0.487 ± 0.021
2.247ArgAsp: 2.247 ± 0.048
2.689ArgGlu: 2.689 ± 0.053
2.18ArgPhe: 2.18 ± 0.047
2.464ArgGly: 2.464 ± 0.051
1.242ArgHis: 1.242 ± 0.034
3.363ArgIle: 3.363 ± 0.057
2.688ArgLys: 2.688 ± 0.058
5.088ArgLeu: 5.088 ± 0.08
1.178ArgMet: 1.178 ± 0.03
1.916ArgAsn: 1.916 ± 0.044
1.527ArgPro: 1.527 ± 0.037
2.187ArgGln: 2.187 ± 0.049
2.18ArgArg: 2.18 ± 0.05
2.526ArgSer: 2.526 ± 0.049
2.017ArgThr: 2.017 ± 0.039
2.871ArgVal: 2.871 ± 0.048
0.632ArgTrp: 0.632 ± 0.025
1.844ArgTyr: 1.844 ± 0.036
0.0ArgXaa: 0.0 ± 0.0
Ser
5.292SerAla: 5.292 ± 0.065
0.741SerCys: 0.741 ± 0.032
3.415SerAsp: 3.415 ± 0.057
3.666SerGlu: 3.666 ± 0.058
2.884SerPhe: 2.884 ± 0.058
4.892SerGly: 4.892 ± 0.076
1.512SerHis: 1.512 ± 0.041
4.563SerIle: 4.563 ± 0.068
3.243SerLys: 3.243 ± 0.054
6.815SerLeu: 6.815 ± 0.082
1.544SerMet: 1.544 ± 0.033
2.49SerAsn: 2.49 ± 0.06
2.413SerPro: 2.413 ± 0.042
2.457SerGln: 2.457 ± 0.053
2.853SerArg: 2.853 ± 0.055
4.138SerSer: 4.138 ± 0.078
3.146SerThr: 3.146 ± 0.061
4.193SerVal: 4.193 ± 0.064
0.873SerTrp: 0.873 ± 0.029
1.989SerTyr: 1.989 ± 0.047
0.0SerXaa: 0.0 ± 0.0
Thr
4.419ThrAla: 4.419 ± 0.057
0.509ThrCys: 0.509 ± 0.023
2.813ThrAsp: 2.813 ± 0.055
3.279ThrGlu: 3.279 ± 0.052
1.997ThrPhe: 1.997 ± 0.049
4.258ThrGly: 4.258 ± 0.068
1.308ThrHis: 1.308 ± 0.031
3.435ThrIle: 3.435 ± 0.06
2.305ThrLys: 2.305 ± 0.051
6.153ThrLeu: 6.153 ± 0.078
1.088ThrMet: 1.088 ± 0.034
1.808ThrAsn: 1.808 ± 0.043
2.589ThrPro: 2.589 ± 0.054
2.317ThrGln: 2.317 ± 0.044
2.324ThrArg: 2.324 ± 0.045
2.97ThrSer: 2.97 ± 0.055
2.581ThrThr: 2.581 ± 0.047
3.592ThrVal: 3.592 ± 0.067
0.559ThrTrp: 0.559 ± 0.023
1.466ThrTyr: 1.466 ± 0.04
0.0ThrXaa: 0.0 ± 0.0
Val
5.441ValAla: 5.441 ± 0.082
0.812ValCys: 0.812 ± 0.028
3.716ValAsp: 3.716 ± 0.064
3.687ValGlu: 3.687 ± 0.069
3.023ValPhe: 3.023 ± 0.055
3.944ValGly: 3.944 ± 0.077
1.318ValHis: 1.318 ± 0.035
4.944ValIle: 4.944 ± 0.066
3.473ValLys: 3.473 ± 0.075
6.707ValLeu: 6.707 ± 0.086
1.657ValMet: 1.657 ± 0.038
2.809ValAsn: 2.809 ± 0.059
2.426ValPro: 2.426 ± 0.045
2.408ValGln: 2.408 ± 0.051
2.646ValArg: 2.646 ± 0.054
4.355ValSer: 4.355 ± 0.065
3.551ValThr: 3.551 ± 0.062
4.202ValVal: 4.202 ± 0.074
0.758ValTrp: 0.758 ± 0.025
1.841ValTyr: 1.841 ± 0.042
0.0ValXaa: 0.0 ± 0.0
Trp
0.833TrpAla: 0.833 ± 0.026
0.161TrpCys: 0.161 ± 0.013
0.593TrpAsp: 0.593 ± 0.022
0.629TrpGlu: 0.629 ± 0.022
0.604TrpPhe: 0.604 ± 0.026
0.748TrpGly: 0.748 ± 0.028
0.365TrpHis: 0.365 ± 0.02
0.784TrpIle: 0.784 ± 0.026
0.544TrpLys: 0.544 ± 0.025
1.744TrpLeu: 1.744 ± 0.037
0.326TrpMet: 0.326 ± 0.017
0.531TrpAsn: 0.531 ± 0.022
0.461TrpPro: 0.461 ± 0.021
0.986TrpGln: 0.986 ± 0.035
0.627TrpArg: 0.627 ± 0.023
0.743TrpSer: 0.743 ± 0.026
0.511TrpThr: 0.511 ± 0.022
0.828TrpVal: 0.828 ± 0.028
0.173TrpTrp: 0.173 ± 0.012
0.366TrpTyr: 0.366 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.663TyrAla: 2.663 ± 0.046
0.435TyrCys: 0.435 ± 0.021
1.615TyrAsp: 1.615 ± 0.041
1.532TyrGlu: 1.532 ± 0.035
1.576TyrPhe: 1.576 ± 0.037
1.976TyrGly: 1.976 ± 0.046
0.83TyrHis: 0.83 ± 0.027
2.002TyrIle: 2.002 ± 0.046
1.614TyrLys: 1.614 ± 0.04
3.672TyrLeu: 3.672 ± 0.055
0.671TyrMet: 0.671 ± 0.023
1.182TyrAsn: 1.182 ± 0.037
1.543TyrPro: 1.543 ± 0.037
2.094TyrGln: 2.094 ± 0.043
1.699TyrArg: 1.699 ± 0.042
2.009TyrSer: 2.009 ± 0.044
1.659TyrThr: 1.659 ± 0.038
1.733TyrVal: 1.733 ± 0.038
0.459TyrTrp: 0.459 ± 0.022
1.154TyrTyr: 1.154 ± 0.034
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3608 proteins (1120843 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski