Staphylococcus virus 69
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q4ZDS8|Q4ZDS8_9CAUD Integrase OS=Staphylococcus virus 69 OX=320834 PE=3 SV=1
MM1 pKa = 7.2 TFKK4 pKa = 10.98 NNHH7 pKa = 5.5 NFNEE11 pKa = 4.17 LVLTNEE17 pKa = 5.72 DD18 pKa = 2.87 IRR20 pKa = 11.84 ILKK23 pKa = 9.94 NVLEE27 pKa = 4.87 DD28 pKa = 3.39 AVSVYY33 pKa = 10.99 DD34 pKa = 4.36 EE35 pKa = 4.33 YY36 pKa = 11.73 SVCNEE41 pKa = 3.92 EE42 pKa = 5.59 SDD44 pKa = 4.23 FAYY47 pKa = 10.74 CLLRR51 pKa = 11.84 DD52 pKa = 4.65 LYY54 pKa = 10.59 TLDD57 pKa = 3.74 SLAISSNNVV66 pKa = 2.67
Molecular weight: 7.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.86
IPC2_protein 4.113
IPC_protein 3.986
Toseland 3.808
ProMoST 4.139
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.897
Rodwell 3.834
Grimsley 3.732
Solomon 3.948
Lehninger 3.91
Nozaki 4.101
DTASelect 4.266
Thurlkill 3.859
EMBOSS 3.897
Sillero 4.113
Patrickios 0.782
IPC_peptide 3.948
IPC2_peptide 4.088
IPC2.peptide.svr19 4.005
Protein with the highest isoelectric point:
>tr|Q4ZDU3|Q4ZDU3_9CAUD ORF003 OS=Staphylococcus virus 69 OX=320834 PE=4 SV=1
MM1 pKa = 7.2 LRR3 pKa = 11.84 YY4 pKa = 8.73 WLVVNWMYY12 pKa = 11.25 QYY14 pKa = 10.46 MMPIIQIILSHH25 pKa = 6.8 IIQRR29 pKa = 11.84 SEE31 pKa = 3.92 NQVCQTDD38 pKa = 4.61 CIRR41 pKa = 11.84 TRR43 pKa = 11.84 GVMKK47 pKa = 9.99 LLCTNLLKK55 pKa = 10.5 IITSFTDD62 pKa = 3.17 RR63 pKa = 11.84 KK64 pKa = 10.32 GGRR67 pKa = 11.84 HH68 pKa = 4.85 GG69 pKa = 3.6
Molecular weight: 8.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.242
IPC2_protein 9.253
IPC_protein 9.472
Toseland 9.692
ProMoST 9.56
Dawson 9.984
Bjellqvist 9.809
Wikipedia 10.204
Rodwell 10.16
Grimsley 10.072
Solomon 10.072
Lehninger 10.043
Nozaki 9.926
DTASelect 9.75
Thurlkill 9.838
EMBOSS 10.131
Sillero 9.97
Patrickios 7.497
IPC_peptide 10.058
IPC2_peptide 9.107
IPC2.peptide.svr19 7.963
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
76
0
76
13695
34
1155
180.2
20.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.666 ± 0.444
0.584 ± 0.085
6.28 ± 0.336
7.47 ± 0.534
4.352 ± 0.228
5.447 ± 0.277
1.738 ± 0.158
7.397 ± 0.313
9.164 ± 0.316
8.025 ± 0.314
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.658 ± 0.164
6.849 ± 0.268
2.76 ± 0.214
3.855 ± 0.18
4.104 ± 0.225
5.681 ± 0.235
6.141 ± 0.282
6.177 ± 0.312
1.161 ± 0.171
4.491 ± 0.344
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here