Paenibacillus sp. oral taxon 786 str. D14
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4460 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C6J149|C6J149_9BACL PspC domain protein OS=Paenibacillus sp. oral taxon 786 str. D14 OX=621372 GN=POTG_01996 PE=4 SV=1
MM1 pKa = 7.61 LIVSEE6 pKa = 4.24 IVAFAEE12 pKa = 4.94 GVGHH16 pKa = 6.99 FYY18 pKa = 10.88 GAEE21 pKa = 3.93 FTRR24 pKa = 11.84 NVAAEE29 pKa = 4.08 PSEE32 pKa = 3.82 ISVPGGDD39 pKa = 3.74 FVTSATSSRR48 pKa = 11.84 FTFPKK53 pKa = 10.29 LGSACDD59 pKa = 3.5 SGQLIVFLDD68 pKa = 3.74 AVSTEE73 pKa = 4.06 DD74 pKa = 3.79 CADD77 pKa = 3.87 AEE79 pKa = 4.54 VSSYY83 pKa = 11.53
Molecular weight: 8.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.802
IPC2_protein 4.012
IPC_protein 3.872
Toseland 3.706
ProMoST 4.024
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.77
Rodwell 3.719
Grimsley 3.617
Solomon 3.821
Lehninger 3.783
Nozaki 3.986
DTASelect 4.113
Thurlkill 3.757
EMBOSS 3.77
Sillero 3.986
Patrickios 1.888
IPC_peptide 3.821
IPC2_peptide 3.973
IPC2.peptide.svr19 3.905
Protein with the highest isoelectric point:
>tr|C6IWV2|C6IWV2_9BACL Nucleoid occlusion protein OS=Paenibacillus sp. oral taxon 786 str. D14 OX=621372 GN=noc PE=3 SV=1
MM1 pKa = 7.59 KK2 pKa = 8.7 PTFKK6 pKa = 10.81 PNVSKK11 pKa = 10.8 RR12 pKa = 11.84 KK13 pKa = 8.95 KK14 pKa = 8.25 VHH16 pKa = 5.49 GFRR19 pKa = 11.84 KK20 pKa = 10.01 RR21 pKa = 11.84 MSTKK25 pKa = 10.18 NGRR28 pKa = 11.84 KK29 pKa = 8.87 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 LKK37 pKa = 10.2 GRR39 pKa = 11.84 KK40 pKa = 8.79 VLSAA44 pKa = 4.05
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.474
IPC2_protein 11.082
IPC_protein 12.618
Toseland 12.808
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.261
Rodwell 12.705
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.793
DTASelect 12.793
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.427
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.035
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4460
0
4460
1399382
22
1883
313.8
34.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.479 ± 0.046
0.744 ± 0.01
5.029 ± 0.028
7.082 ± 0.043
4.037 ± 0.027
7.651 ± 0.032
2.045 ± 0.017
6.403 ± 0.035
5.201 ± 0.031
10.295 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.75 ± 0.016
3.597 ± 0.028
4.251 ± 0.026
3.854 ± 0.026
5.475 ± 0.033
5.87 ± 0.03
5.334 ± 0.031
7.13 ± 0.028
1.318 ± 0.014
3.456 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here